Background and Aim: Infectious coryza (IC) is an upper respiratory disease of chicken caused by Avibacterium paragallinarum. Its clinical symptoms are swollen face and malodorous sinus exudate. This study was conducted to determine the antimicrobial sensitivity of A. paragallinarum isolates from layers in the Special Region of Yogyakarta, Indonesia. Materials and Methods: The samples used in this study were 30 layers that showed IC symptoms. The colony and cell morphology were observed with Gram staining; then, biochemical tests (catalase, oxidase, urease, indole, and motility tests, and carbohydrate fermentation tests using lactose, maltose, mannitol, and sorbitol) were performed to the suspected colony to identify A. paragallinarum. An antibiotic sensitivity test was performed using several antibiotic disks against A. paragallinarum isolates that were cultured on Mueller-Hinton Agar. Results: Out of 30 samples, 24 samples (80%) were found positive for A. paragallinarum. All isolates were sensitive to ampicillin (AMP) and amoxicillin (AML) (100%), and chloramphenicol (C) (91.6%). The antibiotics with intermediate sensitivity were enrofloxacin (79.2%), fosfomycin (75%), and ciprofloxacin (54.2%). The isolates were most resistant to erythromycin (100%), followed by tetracycline (87.5%), streptomycin (83.3%), doxycycline and kanamycin (70.8%), and trimethoprim (62.5%). Conclusion: Out of the total samples, 24 samples (80%) from layers with IC symptoms were identified biochemically as A. paragallinarum. It was sensitive to AMP, AML, and C.

With the prohibition of the use of Antibiotic growth promotors (AGP) used in animal feed, especially in broilers, the use of feed additives such as herbs, probiotics, prebiotics, synbiotics or mixtures of some of these ingredients is currently widely used as feed replacements for AGP substitutes. One of the commercial feed additives that contain combinations herbal and probiotics is Promix®. In broiler maintenance, one vaccine that must be given is Newcastle Disease (ND) vaccine. The purpose of this study was to determine the role of supplementation of commercial feed combination of herbs and probiotics in broilers given ND vaccines. A total of 42 broilers were divided into two, group A (vaccinated with ND) and B (not vaccinated ND), each with 21 heads. Group A is divided into groups A1, A2 and A3 each with 7 heads. The A1 group is given only basal feed; A2 basal feed and AGP; A3 basal feed and Promix®. Group B is divided by B1, B2 and B3 same as group A before. Broilers are maintained for 5 weeks and weighing each week. All data obtained were statistically analyzed using One Way ANOVA and continued with Duncan's post hoc test. The results of this study were that the group of chickens given the ND vaccine and given supplements of Promix® had greater weight gain (P˂0.05) compared to the AGP and basal groups in the fifth week while in the group of chickens that were not given the ND vaccine but given supplement Promix® feed had greater weight gain (P˂0.05) compared to the AGP and basal groups in the fourth week. In the fifth week there was no significant difference (P˂0.05) between all feed groups in the group not given the ND vaccine. The conclusion of this study that feed additives contain combinations herbal and probiotics can replace AGP as feed additive in broiler. By giving ND vaccine the weight gain of the feed group plus Promix® showed a significant increase in weight gain.

Avian pathogenic Escherichia coli (APEC) is a cause of colibacillosis in poultry, one of the respiratory disease that causes serious problems in the poultry industry. The APEC can cause high mortality and culling, decreased production, and high costs of treatment. Manifestations of colibacillosis are airsacculitis, perihepatitis, and pericarditis. The APEC serotypes that are widely identified in the field are O1K1, O2K1, and O78K80. Embryo lethality assay (ELA) is a method for determine the virulence of APEC serotypes. The aim of this study was to determine the virulence characteristic of APEC isolates. Five APEC serotypes O1K1, O2K1, O78K80, O157H7, and unknown serotype were used for ELA method by inoculated E. coli into chorioallantoic of specific pathogen free 12-days old embryos. Each group of 10 embryos, inoculated E. coli dose of 100-500 CFU/ 0,1 ml. Candling was carried out for 6 days (18-days old embryo) to determined the mortality and pathological lesions. The percentage of embryo mortality post-inoculated with APEC O1K1, O2K1, unknown serotypes were 100% (10/10), O78K80 serotype was 90% (9/10), and O157H7 serotype was 70% (70%). Lesions of all embryos were cranial and extremity hemorrhage. In this study, E. coli isolates had high virulence.

Abstract. Fauziah I, Asmara W, Wahyuni AETH, Widayanti R. 2021. Short Communication: PCR detection of Avibacterium paragallinarum from layers with infectious coryza symptoms in poultry farms of Sleman District, Indonesia. Biodiversitas 22: 4890-4894. Infectious coryza (IC), caused by Avibacterium paragallinarum, is a contagious and infectious respiratory tract disease that affects the commercial poultry industry. Molecular techniques, such as species-specific PCR, HPG-2 PCR are mostly used for the detection of  A. paragallinarum. The current research was carried out to isolate A. paragallinarum from the layers of infectious coryza signs in Sleman District, special region of Yogyakarta, followed by PCR confirmation of the identified bacteria. Nine field isolates were observed and determined based on their colony and cell morphology. All isolates were characterized biochemically and confirmed with species-specific HPG-2 PCR for A. paragallinarum. Out of 9 isolates, 6 (66.7%) isolates were biochemically identified as A. paragallinarum and confirmed by HPG-2 PCR.