Efficacy of a Peroxyacetic Acid Formulation as an Antimicrobial Intervention To Reduce Levels of Inoculated Escherichia coli O157:H7 on External Carcass Surfaces of Hot-Boned Beef and Veal

Abstract

The efficacy of a peroxyacetic acid formulation (POAA) at reducing Escherichia coli O157:H7 contamination on external carcass surfaces of hot-boned beef and veal with a commercial spray apparatus was determined. Hot-boned external carcass surfaces were inoculated with either a high dose (10(6) CFU/cm2) in fresh bovine feces or with a low dose (10(3) CFU/cm2) in diluent of laboratory-cultured E. coli O157:H7. Treatments included a water wash, a POAA (180 ppm) wash, or a water plus POAA wash. Samples were extracted from the external carcass surface with a cork borer to determine the numbers of viable E. coli O157:H7 remaining on the carcass surface after treatment. Although a water wash alone resulted in a 1.25 (94.4%) and a 1.31 (95.1%) mean log reduction on veal and beef inoculated with a high dose of E. coli O157:H7, the POAA treatment resulted in a substantially greater mean log reduction of 3.56 and 3.59 (>99.9%). The water wash only resulted in a 33.9% reduction on veal and 62.8% on beef inoculated with a low dose of E. coli O157:H7, whereas POAA treatment greatly improved pathogen reduction to 98.9 and 97.4% on veal and beef, respectively. The combination of a water wash followed by a POAA treatment resulted in a similar E. coli O157:H7 reduction to that achieved by POAA treatment alone. In conclusion, POAA treatment significantly reduced viable E. coli O157:H7 numbers on experimentally contaminated beef and veal carcasses, which justifies its use as a chemical intervention for the removal of this human pathogen.

Reduction of Surrogates for Escherichia coli O157:H7 and Salmonella during the Production of Nonintact Beef Products by Chemical Antimicrobial Interventions

“…When counts were below the detection limit (2.0 log CFU/20 cm 2) of the method, a number between 0 and the lowest detection limit was used to facilitate the data analysis. differs from results reported by Penney et al (26), who found greater reductions after PAA compared with water applica tion. In contrast, King et al (18) achieved greater reductions of E. coli 0157:H7 and Salmonella Typhimurium with a water wash than with PAA.…”

Antibacterial activity of decontamination treatments for cattle hides and beef carcasses

“…However, supplementary hot water spraying (95 °C) did not further enhance the efficacy (Castillo et al, 1999a). Besides, in the study of Phebus et al (1997) Occasionally, the antibacterial activity of water spraying in combination with chemicals as ASC, chlorine, H 2 O 2 , ozone, peroxyacetic acid, saponin, TSP or commercially available preparations was investigated on inoculated beef carcass surface parts (Cabedo et al, 1996;Castillo et al, 1999b;Castillo et al, 2003;Cutter, 1999;Gorman et al, 1995b;Marshall et al, 2005;Penney et al, 2007). The different chemicals were mainly investigated in only one single study and under laboratory conditions.…”

Carcass decontamination methods in slaughterhouses: a review

“…Furthermore, a lot of research has been carried out on the effectiveness of various commercially available preparations, (Cutter and Siragusa, 1995;Reagan et al, 1996;Bell et al, 1997;Dorsa et al, 1997;Cutter, 1999;Cutter and Rivera-Betancourt, 2000;Bosilevac et al, 2004;Gill and Badoni, 2004;King et al, 2005;Algino et al, 2007;Penney et al, 2007;Arthur et al, 2008;Pearce and Bolton, 2008;Kalchayanand et al, 2009).…”

“…This chemical received approval from FSIS -USDA for use in beef carcass decontamination systems. On the other hand, acidified sodium chlorite, electrolyzed water or peroxyacetic acid mainly yielded reductions below one order of magnitude (Algino et al, 2007;Arthur et al, 2008;Gill and Badoni, 2004;King et al, 2005;Penney et al, 2007). Under commercial conditions, H 2 O 2 and ozone reduced naturally occurring bacteria on carcasses by 1.0-1.1 and 1.1-1.3 log CFU cm -2 , respectively (Reagan et al, 1996;Algino et al, 2007).…”