Efficacy of a Probiotic‐Prebiotic Supplement on Incidence of Diarrhea in a Dog Shelter: A Randomized, Double‐Blind, Placebo‐Controlled Trial

Rose, Lindsay; Rose, Jennifer; Gosling, Sarah E A; Holmes, Mark A.

doi:10.1111/jvim.14666

Cited by 41 publications

(51 citation statements)

References 12 publications

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“…A previous study for instance showed varying microbial changes in kenneled dogs depending on the dosage of the probiotic treatment [ 17 ]. Several studies showed an improvement in clinical signs by probiotic treatment such as causing significant decreased incidence of diarrhea in sheltered dogs and a significant reduction in duration of uncomplicated acute diarrhea in dogs [ 18 – 20 ]. Dogs with idiopathic inflammatory bowel disease treated with probiotics in addition to standard therapy showed an enhancement of regulatory T-cell markers, normalization of dysbiosis, and up-regulated expression of tight junction proteins [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of probiotic treatment on the clinical course, intestinal microbiome, and toxigenic Clostridium perfringens in dogs with acute hemorrhagic diarrhea

Ziese¹,

Suchodolski

Hartmann³

et al. 2018

PLoS ONE

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IntroductionThe impact of probiotics on dogs with acute hemorrhagic diarrhea syndrome (AHDS) has not been evaluated so far. The study aim was to assess the effect of probiotic treatment on the clinical course, intestinal microbiome, and toxigenic Clostridium perfringens in dogs with AHDS in a prospective, placebo-controlled, blinded trial.MethodsTwenty-five dogs with AHDS with no signs of sepsis were randomly divided into a probiotic (PRO; Visbiome, ExeGi Pharma) and placebo group (PLAC). Treatment was administered for 21 days without antibiotics. Clinical signs were evaluated daily from day 0 to day 8. Key bacterial taxa, C. perfringens encoding NetF toxin and enterotoxin were assessed on days 0, 7, 21.ResultsBoth groups showed a rapid clinical improvement. In PRO a significant clinical recovery was observed on day 3 (p = 0.008), while in PLAC it was observed on day 4 (p = 0.002) compared to day 0. Abundance of Blautia (p<0.001) and Faecalibacterium (p = 0.035) was significantly higher in PRO on day 7 compared to day 0, while in PLAC the abundance of Faecalibacterium was not significantly higher on any study day and Blautia (p = 0.016) was only significantly higher on day 21 compared to day 0. Abundance of C. perfringens was significantly lower on day 7 (p = 0.011) compared to day 0 in PRO but not in PLAC. Enterotoxin genes were significantly lower in PRO on day 21 (p = 0.028) compared to PLAC. Fecal samples of 57% of all dogs were positive for netF toxin genes on day 0 and the abundance was significantly lower on day 7 compared to day 0 in PRO (p = 0.016) and PLAC (p = 0.031).ConclusionThe probiotic treatment was associated with an accelerated normalization of the intestinal microbiome. Dogs with aseptic AHDS showed a rapid decrease of netF toxin genes and fast clinical recovery in both groups under symptomatic treatment without antibiotics.

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Section: Introductionmentioning

confidence: 99%

Effect of probiotic treatment on the clinical course, intestinal microbiome, and toxigenic Clostridium perfringens in dogs with acute hemorrhagic diarrhea

Ziese¹,

Suchodolski

Hartmann³

et al. 2018

PLoS ONE

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“…Given these premises, to maintain the correct equilibrium in the GI microbiota, human and animal medicine have increased the use of prebiotic, probiotic or synbiotic (the combination of the two) and faecal microbiota transplantation (FMT) (Rose et al 2017;Pilla and Suchodolski 2019;Whittemore et al 2019). The higher short chain fatty acids production due to an increasing fermentation activity in large intestine of dogs (Musco et al 2018) and cats (Calabr o et al 2020), affects energy balance (Deng and Swanson 2015;Fluitman et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Study of faecal parameters and body condition in dogs with a diet supplemented with Lactobacillus acidophilus D2/CSL (CECT 4529)

Bruni¹,

Martello

Fusi

et al. 2020

Italian Journal of Animal Science

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The aim of our case-control study is to evaluate the effects of a diet integrated with the probiotic Lactobacillus acidophilus D2/CSL (CECT 4529) on the nutritional status and faecal consistency of healthy dogs belonging to the English Cocker Spaniel (ECS) and Labrador Retriever (LR) breeds. A total of 30 dogs were enrolled in this study, and they were randomly assigned to a Control (CTR, n ¼ 14) and a Treated group (LACTO, n ¼ 16). The trial consisted in a 7-days adaptation period where all the animals received the same commercial food, followed by a 35-days of data collection period where the LACTO group received the food supplemented with Lactobacillus acidophilus D2/CSL. We evaluated Body weight (BW), Body Condition Score (BCS) and Skinfold thickness, Faecal Score (FS) and Faecal Moisture (FM). All dogs in the LACTO group maintained an ideal BCS score during the whole experimental period compared to the CTR group. A significant decrease in skin thickness was found throughout the trial in the LACTO group. A significant improvement of the FM was recorded in the LACTO compared to the CTR group in the overall period for both dog breeds, and the FS significantly decreased in the LACTO group. Our results showed good maintenance of the nutritional conditions in dogs that are prone to overweight and a significant improvement of faecal parameters, meaning that even in healthy dogs with no gastrointestinal disorder the addition of this supplement to the diet helps to maintain the optimal balance of their intestinal microbiota. HIGHLIGHTS Probiotics help to maintain and promote the optimal gastro-intestinal health and well-being. Lactobacillus acidophilus D2/CSL (CECT 4529) improve faecal parameters and nutritional status of dogs.

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“…These dogs did not have reported clinical symptoms of GI diseases, so the clinical impact of this change by SN is unknown. It is intriguing to speculate that the presence of these pathogens may indicate increased susceptibility or predisposition to developing GI disorders or diseases, and that SN treatment may have a prophylactic effect by reducing their abundances as previously reported [60][61][62] . Consistent with this was our finding that the PL group had a non-significant trend toward higher incidence of diarrhea than SN.…”

Section: Discussionmentioning

confidence: 87%

Gut microbes in healthy dogs have individualized responses to synbiotic supplementation that lead to increased abundances of probiotic strains: A randomized controlled trial

Tanprasertsuk¹,

Jha²,

Shmalberg³

et al. 2020

Preprint

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Background: Probiotics ameliorate gastrointestinal symptoms in dogs. However, the effect of probiotics in a healthy population, as well as factors contributing individualized responses, remains largely unknown. This trial examined gut microbiota (GM) and health outcomes in household dogs after synbiotic (SN) supplementation containing probiotics and inulin. Healthy dogs were randomized to receive SN (50 mg/d inulin and 20 billion total CFU/d of L. reuteri, P. acidilactici, E. faecium, L. acidophilus, B. animalis, L. fermentum, L. rhamnosus) or placebo (PL) for 4 weeks. Owners completed a health survey and collected stool samples for GM profiling (metagenomic sequencing) at baseline and week 4 in both groups, and at week 6 in the SN group.Results: A significant shift (p<0.001) in β-diversity was observed in the SN (n=24), but not PL group (n=19), relative to baseline at week 4. Forty-five bacterial species, 43 (96%) of which were Lactobacillales, showed an increase in the abundances (≥2 fold change, adjusted p<0.05) at week 4. E. coli also decreased at week 4 in the SN group (2.8 folds, adjusted p<0.01). The altered taxa largely returned to baseline at week 6. The degree of changes in the β-diversity was associated with GM at baseline. Specifically, dogs with higher Proteobacteria and lower Lactobacillales responded more robustly to supplementation. Dogs fed SN tended to have lower diarrhea incidence (0% vs 16%, p=0.08).Conclusions: SN supplement had an impact on the change of gut microbiota in healthy household dogs as characterized with metagenomic sequencing. Findings warrant further investigation with longer duration or in populations at risk of gastrointestinal diseases. The magnitude of response to the supplement was associated with microbial profile at baseline. To our knowledge, this is the first study documenting such association that may provide a basis of personalized nutrition in companion dogs.

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Efficacy of a Probiotic‐Prebiotic Supplement on Incidence of Diarrhea in a Dog Shelter: A Randomized, Double‐Blind, Placebo‐Controlled Trial

Cited by 41 publications

References 12 publications

Effect of probiotic treatment on the clinical course, intestinal microbiome, and toxigenic Clostridium perfringens in dogs with acute hemorrhagic diarrhea

Effect of probiotic treatment on the clinical course, intestinal microbiome, and toxigenic Clostridium perfringens in dogs with acute hemorrhagic diarrhea

Study of faecal parameters and body condition in dogs with a diet supplemented with Lactobacillus acidophilus D2/CSL (CECT 4529)

Gut microbes in healthy dogs have individualized responses to synbiotic supplementation that lead to increased abundances of probiotic strains: A randomized controlled trial

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