Efficacy of a Tyrothricin-Containing Wound Gel in an Abrasive Wound Model for Superficial Wounds

Background: The healing of wounds has always provided challenges for the medical community whether chronic or acute. Understanding the processes which enable wounds to heal is primarily carried out by the use of models, in vitro, animal and human. It is generally accepted that the use of human models offers the best opportunity to understand the factors that influence wound healing as well as to evaluate efficacy of treatments applied to wounds. Objectives: The objective of this article is to provide an overview of the different methodologies that are currently used to experimentally induce wounds of various depths in human volunteers and examines the information that may be gained from them. Methods: There is a number of human volunteer healing models available varying in their invasiveness to reflect the different possible depth levels of wounds.Results: Currently available wound healing models include sequential tape stripping, suction blister, abrasion, laser, dermatome, and biopsy techniques. The various techniques can be utilized to induce wounds of variable depth, from removing solely the stratum corneum barrier, the epidermis to even splitthickness or full thickness wounds. Conclusion: Depending on the study objective, a number of models exist to study wound healing in humans. These models provide efficient and reliable results to evaluate treatment modalities. I N VITRO studies and animal models have provided useful information for many breakthroughs and medical advances. However, these are not always appropriate and in many cases are inadequate for discovering the actual pathology responsible. While animal models are suitable for many skin disorders and diseases, they do have their limitations when wound healing is being investigated. The complex biological pathway that occurs during wound healing is not always mirrored in animal models and human models need to be considered. In the case of chronic wound pathology, human models are not always good predictors but they do provide a good understanding of acute wound pathology. Because of the difficulty in obtaining suitable volunteers with chronic wounds and incorporating them into a study, human acute wound models provide the best opportunity to understand the wound healing process and the performance of different products that assist and promote wound healing. This paper evaluates the different wound healing models and discusses the potential for these models to be suitable predictors of wound healing.

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“…This model has also more recently been used to evaluate the effects of a topical antimicrobial gel and its vehicle on wound healing (30).…”

Section: Animal Models Of Wound Healingmentioning

confidence: 99%

Models of wound healing: an emphasis on clinical studies

Wilhelm

Wilhelm²,

Bielfeldt³

2016

Skin Research and Technology

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“…Nanoparticles are already present in a range of consumer products in everyday use, such as cosmetics or clothes, and in a number of medical fields, such as oncology and cardiovascular medicine [66,67,68,69]. The increasing popularity of nanotechnology in the latter is closely related to the emerging concept of particulate delivery systems.…”

Section: Future Challenges To Improving Skin Antisepsismentioning

confidence: 99%

“…If water soluble, the artificially added active compounds can be located in the aqueous spaces within the lipid membrane. If lipid soluble, they can be attached to the lipid membranes [66,68,69]. …”

Section: Future Challenges To Improving Skin Antisepsismentioning

confidence: 99%

New Strategies for Preoperative Skin Antisepsis

Ulmer

Lademann

Patzelt

et al. 2014

Skin Pharmacol Physiol

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During the past decades, encouraging progress has been made in the prevention of surgical site infections (SSI). However, as SSI still occur today, strategic prevention measures such as standardized skin antisepsis must be implemented and rigorously promoted. Recent discoveries in skin physiology necessitate the development of novel antiseptic agents and procedures in order to ameliorate their efficacy. In particular, alternate target structures in the skin need to be taken into consideration for the development of the next generation of antiseptics. Recent investigations have shown that a high number of microorganisms are located within and in the close vicinity of the hair follicles. This suggests that these structures are an important reservoir of bacterial growth and activity in human skin. To date, it has not been fully elucidated to what extent conventional liquid antiseptics sufficiently target the hair follicle-related microbial population. Modern technologies such as tissue-tolerable plasma (TTP) have been tested for their potential antiseptic efficiency by reducing the bacterial load in the skin and in the hair follicles. First experiments using liposomes to deliver antiseptics into the hair follicles have been evaluated for their potential clinical application. The present review evaluates these two innovative methods for their efficacy and applicability in preoperative skin antiseptics.

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“…At a 10% concentration, the consistency is comparable to that of petrolatum. Therefore, a vehicle control [for example, see [23]] is unable to blind the treatment regime. Furthermore, pure, liquid sunflower oil on an adjacent wound area would spread over to the test area and the TE would diffuse the other way, jeopardizing the test design.…”

Section: Discussionmentioning

confidence: 99%

Accelerated Reepithelialization by Triterpenes: Proof of Concept in the Healing of Surgical Skin Lesions

Metelmann

Brandner

Schumann

et al. 2014

Skin Pharmacol Physiol

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The acceleration of wound healing is a major surgical concern. A triterpene extract from birch bark (Betulae cortex) experimentally enhances keratinocyte differentiation in vitro and accelerates wound healing ex vivo. We conducted an open, blind-evaluated, controlled, prospective, randomized (1:1) phase II clinical trial in patients requiring split-thickness skin graft transplantation at two university hospitals in Germany. Donor sites on the upper legs were covered with a moist silicone-coated dressing. Oleogel-S10 ointment containing 10% birch bark extract was randomly applied to the distal or proximal half of the wound, with the other half serving as an intraindividual control, for 14 days after the skin graft surgery. The primary efficacy variable was faster reepithelialization as determined from macrophotographs by independent, blinded experts. Twenty-four patients were randomized and completed the trial. After the 14-day test period, the planned interim analysis revealed a highly significant (p 0.0001) superiority of Oleogel-S10 in the primary efficacy variable and the trial was terminated early due to ethical concerns. The treatment side was also better reepithelialized and more similar to normal skin after 3 months. In conclusion, Oleogel-S10 significantly accelerated reepithelialization at split-thickness skin graft donor sites. Treatment with Oleogel-S10 was safe and well tolerated. i 2014 S. Karger AG, Basel

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Efficacy of a Tyrothricin-Containing Wound Gel in an Abrasive Wound Model for Superficial Wounds

Cited by 22 publications

References 38 publications

Models of wound healing: an emphasis on clinical studies

Models of wound healing: an emphasis on clinical studies

New Strategies for Preoperative Skin Antisepsis

Accelerated Reepithelialization by Triterpenes: Proof of Concept in the Healing of Surgical Skin Lesions

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