Efficacy of a Whey Protein Concentrate on the Inhibition of Stomach Ulcerative Lesions Caused by Ethanol Ingestion

Abstract

Objetivo Avaliar a atividade do hidrolisado das proteínas de soro de leite bovino e uma fração de peptídeos de baixo peso molecular (peso molecular ≤1kDa), na proteção do epitélio da mucosa do estômago de ratos Wistar adultos contra o processo ulcerativo, induzidos por três diferentes agentes. Métodos Nesse estudo foram utilizados os modelos de indução de úlcera pelo antiinflamatório indometacina (30mg/kg de peso), por etanol absoluto (1ml/animal) e por estresse causado por imobilização e frio (4 o C/2h) em ratos Wistar adultos. Resultado O hidrolisado protéico de soro de leite foi obtido por tratamento com pancreatina, ao grau de hidrólise de 20%, e fracionado em membrana de fluxo tangencial com faixa de corte de 1kDa, para obtenção de uma fração contendo peptídeos de baixo peso molecular denominada peptídeos do hidrolisado protéico de soro (≤1kDa). A administração aguda do hidrolisado protéico de soro, de acordo com o modelo etanol, resultou em 65,5% de redução dos índices de lesões ulcerativas, sendo obtida 77,4% de inibição em dose dupla.

Antiulcerative properties of bovine α-lactalbumin

“…Lab.aLa, Comm.a-La, a pancreatin hydrolysate ($20% DH) of Comm.a-La and two hydrolysate fractions, F 1 (o1 kDa) and F 2 (41 kDa), decreased the ULI in the range of 32.2-49.8% as compared with the control, without statistical differences (p40.05) among them while the drug carbenoxolone lowered the ULI by 91%. It has been verified (Rosaneli et al, 2002) that the administration of two consecutive daily doses of 1000 mg kg À1 bw WPC reduced the ULI caused by 1 mL absolute ethanol by 73% while carbenoxolone (200 mg kg À1 bw) reduced it by 86.5%, based on physiological saline as the control.…”

“…Studies reported recently (Rosaneli, Bighetti, Antoˆnio, Carvalho, & Sgarbieri, 2002, 2004) with a WPC produced in a pilot plant (Zinsly et al, 2001) and with a Comm.a-La (Matsumoto et al, 2001) demonstrated that whey proteins were capable of inhibiting the development of ulcerative lesions in the rat stomach mucosa by various ulcerogenic agents. Rosaneli et al (2004) used the same WPC, which was used in the present work to isolate a-La, and reported a 50% decrease of the ULI when two consecutive daily doses of 1000 mg kg À1 bw were administered to the rat, in the indomethacin model.…”

“…Our group (Rosaneli et al, 2002(Rosaneli et al, , 2004 showed the importance of sulfhydryl substances in the protection of Table 2 Effect of a-lactalbumin produced in the laboratory (Lab.a-La), commercial a-lactalbumin (Comm.a-La) and two Comm.a-La hydrolysates (F 1 and F 2 ), and carbenoxolone on the gastric production of mucus a Intragastric intubation Table 3 Effect of a-lactalbumin produced in the laboratory (Lab.a-La), a commercial a-lactalbumin (Comm.a-La) and two Comm.a-La hydrolysates (F 1 and F 2 ), indomethacin, and carbenoxolone on the biosynthesis of prostaglandin (PGE 2 ) in the rat gastric mucosa gastric mucosa against indomethacin and absolute ethanol. The combination of saline plus NEM permitted to estimate the protection offered to the gastric mucosa by naturally occurring sulfhydryl substances.…”

Therapeutic Potential of Milk Whey

“…In a research by Rosaneli et al [51], it was observed that when rats showed a 41% reduction in ulcerative lesions caused by ethanol ingestion when they were fed a whey protein concentrate, while a 73% reduction rate was observed following repeat doses of whey (McGregor and Poppitt, 2013 [52]). …”

Future Challenges of Whey Proteins

“…This effect is thought to be related to the sulfhydryl component, particularly cysteine and its link with glutamic acid in the production of glutathione. In a study by Rosaneli et al (2002), rats fed a whey protein concentrate showed a 41% reduction in ulcerative lesions caused by ethanol ingestion while, a 73% reduction rate was observed following repeat doses of whey (Marshall, 2004;McGregor and Poppitt, 2013).…”