Efficacy of an intron-containing kanamycin resistance gene as a selectable marker in plant transformation

Libiakova, G.; Jørgensen, Bodil; Palmgren, Gorm; Ulvskov, Peter; Johansen, Eric

doi:10.1007/s002990100375

Cited by 22 publications

(19 citation statements)

References 27 publications

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“…Typically, transgenic cassettes introduced for heterologous expression in plant cells carry either prokaryotic genes or cDNAs of eukaryotic genes. Aside from processing of 5Ј introns used as transcriptional enhancers in monocot transgene cassettes (Callis et al 1987;Maas et al 1991) or introns used to Designations above the bars, single asterisk (*), double asterisks (**) and triple asterisks (***), correspond to statistically signiWcant diVerences at P = 0.05, 0.005 and 0.0001, respectively prevent A. tumefaciens expression of visual or selectable marker genes (Libiakova et al 2001;Vancanneyt et al 1990) minimal information is available on processing of introns from transgenic transcripts. Transgenic soybean events in which the co-expression of FRO2 was observed, accumulated both processed and unprocessed transcripts at approximately equal levels (Fig.…”

Section: Discussionmentioning

confidence: 99%

Molecular and phenotypic characterization of transgenic soybean expressing the Arabidopsis ferric chelate reductase gene, FRO2

Vasconcelos

Eckert

Arahana

et al. 2006

Planta

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Soybean (Glycine max Merr.) production is reduced under iron-limiting calcareous soils throughout the upper Midwest regions of the US. Like other dicotyledonous plants, soybean responds to iron-limiting environments by induction of an active proton pump, a ferric iron reductase and an iron transporter. Here we demonstrate that heterologous expression of the Arabidopsis thaliana ferric chelate reductase gene, FRO2, in transgenic soybean significantly enhances Fe(+3) reduction in roots and leaves. Root ferric reductase activity was up to tenfold higher in transgenic plants and was not subjected to post-transcriptional regulation. In leaves, reductase activity was threefold higher in the transgenic plants when compared to control. The enhanced ferric reductase activity led to reduced chlorosis, increased chlorophyll concentration and a lessening in biomass loss in the transgenic events between Fe treatments as compared to control plants grown under hydroponics that mimicked Fe-sufficient and Fe-deficient soil environments. However, the data indicate that constitutive FRO2 expression under non-iron stress conditions may lead to a decrease in plant productivity as reflected by reduced biomass accumulation in the transgenic events under non-iron stress conditions. When grown at Fe(III)-EDDHA levels greater than 10 microM, iron concentration in the shoots of transgenic plants was significantly higher than control. The same observation was found in the roots in plants grown at iron levels higher than 32 microM Fe(III)-EDDHA. These results suggest that heterologous expression of an iron chelate reductase in soybean can provide a route to alleviate iron deficiency chlorosis.

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Section: Discussionmentioning

confidence: 99%

Molecular and phenotypic characterization of transgenic soybean expressing the Arabidopsis ferric chelate reductase gene, FRO2

Vasconcelos

Eckert

Arahana

et al. 2006

Planta

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“…Samsun) were obtained as had been described previously (Luka et al 1994). The heterozygous population of the T 1 generation of the transgenic line designated as PHYA antisense was used for the most of the experiments.…”

Section: Plants and Treatmentsmentioning

confidence: 99%

“…The cDNA was obtained by AMV reverse transcriptase (Promega, Madison, USA) and oligo dT 18 primer. The PCR was performed by using LC Taq polymerase, high fidelity buffer (Fermentas/Thermo Fisher Scientific, Vilnius, Lithuania) and the following primers: NPTS (5'-CCTTGCTCCTGCCGAGAAAGTC TCC), NPTA (5'-CGGCAAGCAGGCATCGCCATGG GTC) for nptII gene (Libiakova et al 2001); CUCS (5'-TGCTCAGACATCTGTTGATGCG), CUCA (5'-TGA CCTAGGCTGTTGATCTCCG) for PHYA of Cucurbita pepo (GenBank accession number M15265) and ActS (5'-CATGGTATTGTCAGCAATTG), Act A (5'-CCA CGCTCAGTGAGGATC) for the potato actin gene (GenBank accession number X55749). Annealing was performed at 58 °С for nptII, at 55 °С for PHYA of Cucurbita pepo and at 48 °С for the potato actin gene.…”

Section: Rna-pcr Analysismentioning

confidence: 99%

“…The tobacco transgenic plants containing a genomic cassete for the synthesis of Cucurbita pepo PHYA asRNA were produced earlier (Luka et al 1994). The aim of this study was to show the biological activity of introduced construction by detection of reduced content of PHYA and examining the transgenic plants for far-red responses assumed to be regulated by phytochrome A.…”

Section: Introductionmentioning

confidence: 99%

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Responses of transgenic Nicotiana tabacum seedlings expressing a Cucurbita pepo antisense PHYA RNA to far-red radiation

Гапеева¹,

Antsipava²,

Pundik³

et al. 2011

Biologia plant.

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The Nicotiana tabacum transgenic plants expressing a Cucurbita pepo antisense PHYA RNA were obtained. The seedlings of transgenic tobacco with reduced phytochrome A (PHYA) content displayed decreased sensitivity to continuous broad-band far-red radiation (λ > 680 nm). Under far-red irradiance transgenic seedlings showed less elongation of the hypocotyls, more rapid plastid development, more chlorophyll accumulation, less repression of lightdependent NADPH:protochlorophyllide oxidoreductase than wild-type plants that was in accordance with PHYA control of plant development. Dynamics of the far-red radiation dependent changes in low temperature chlorophyll fluorescence spectra for the transgenic and wild-type seedlings were consistent with the more rapid formation of photosynthetic apparatus in the seedlings with reduced PHYA.

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“…The plantlets were cultivated at 20 ± 2°C with a day length of 16 h under 50 lE m -2 s -1 light intensity. Plants were cultured on basal MS medium (Murashige and Skoog 1962) according to Libiakova et al (2001). Leaf segments of 4-6 weeks old plantlets were transformed with Agrobacterium inoculum according to Moravčíková et al (2003).…”

Section: Potato Transformation and Regenerationmentioning

confidence: 99%

Stress-induced expression of cucumber chitinase and Nicotiana plumbaginifolia β-1,3-glucanase genes in transgenic potato plants

et al. 2007

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The genes encoding for a cucumber class III chitinase and Nicotiana plumbaginifolia class I glucanase were co-introduced into Slovak potato (Solanum tuberosum L.) cultivar ETA using Agrobacterium tumefaciens. Expression of both genes was driven by wound-inducible polyubiquitin promoter isolated from Slovak potato breeding line 116/86. Analyses showed inducible, peel-specific expression of both transgenes under stress conditions. The effect of transgene expression on fungal susceptibility of transformants was evaluated in vitro and in vivo. Experiments with crude protein extracts isolated from transgenic microtubers showed growth inhibition of Rhizoctonia solani hyphae in the range from 7.3 to 14.2%. In contrast, experiments performed in growth chamber conditions revealed that the polyubiquitin promoter driven transgene expression did not ensure any obvious increase of transgenic potato resistance against Rhizoctonia solani.

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Efficacy of an intron-containing kanamycin resistance gene as a selectable marker in plant transformation

Cited by 22 publications

References 27 publications

Molecular and phenotypic characterization of transgenic soybean expressing the Arabidopsis ferric chelate reductase gene, FRO2

Molecular and phenotypic characterization of transgenic soybean expressing the Arabidopsis ferric chelate reductase gene, FRO2

Responses of transgenic Nicotiana tabacum seedlings expressing a Cucurbita pepo antisense PHYA RNA to far-red radiation

Stress-induced expression of cucumber chitinase and Nicotiana plumbaginifolia β-1,3-glucanase genes in transgenic potato plants

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