Efficacy of antimicrobial photodynamic therapy with Rose Bengal and blue light against cariogenic bacteria

Hirose, Marina; Yamaguchi, Yasuo; Horii, Kouichiro; Hasegawa, Yoshinori; Shibuya, Yasuyuki

doi:10.1016/j.archoralbio.2020.105024

Cited by 43 publications

(22 citation statements)

References 36 publications

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“…Rose bengal (RB) dye (4,5,6,7-tetrachloro-2′,4′,5′,7′-tetraiodofluorescein) has been clinically investigated for the treatment of melanoma and other solid cancers [ 10 , 11 , 12 , 13 , 14 ]. Photodynamic antibacterial properties of RB have been reported for a limited number of bacteria [ 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ]. RB is a bright rose-red xanthene compound that was first synthesized in the 19th century as a wool dye and subsequently used as a food dye in Japan (food red no.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Pharmaceutical-Grade Rose Bengal: An Application of a Synthetic Dye in Antibacterial Therapies

et al. 2022

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Rose bengal has been used in the diagnosis of ophthalmic disorders and liver function, and has been studied for the treatment of solid tumor cancers. To date, the antibacterial activity of rose bengal has been sporadically reported; however, these data have been generated with a commercial grade of rose bengal, which contains major uncontrolled impurities generated by the manufacturing process (80–95% dye content). A high-purity form of rose bengal formulation (HP-RBf, >99.5% dye content) kills a battery of Gram-positive bacteria, including drug-resistant strains at low concentrations (0.01–3.13 μg/mL) under fluorescent, LED, and natural light in a few minutes. Significantly, HP-RBf effectively eradicates Gram-positive bacterial biofilms. The frequency that Gram-positive bacteria spontaneously developed resistance to HP-RB is extremely low (less than 1 × 10−13). Toxicity data obtained through our research programs indicate that HP-RB is feasible as an anti-infective drug for the treatment of skin and soft tissue infections (SSTIs) involving multidrug-resistant (MDR) microbial invasion of the skin, and for eradicating biofilms. This article summarizes the antibacterial activity of pharmaceutical-grade rose bengal, HP-RB, against Gram-positive bacteria, its cytotoxicity against skin cells under illumination conditions, and mechanistic insights into rose bengal’s bactericidal activity under dark conditions.

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Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Pharmaceutical-Grade Rose Bengal: An Application of a Synthetic Dye in Antibacterial Therapies

et al. 2022

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“…Recently, aPDT has been introduced as an alternative therapy for preventing and treating dental caries via control the formation of the microbial biofilm and controlling the incidence of microbial pathogens without the development of resistance [29][30][31]. Several variables such as the type and concentration of photosensitizers, light sources, irradiation parameters, and physiologic condition of the microorganisms studied must be considered for the effectiveness of aPDT [32].…”

Section: Discussionmentioning

confidence: 99%

Enhanced reduction of polymicrobial biofilms on the orthodontic brackets and enamel surface remineralization using zeolite-zinc oxide nanoparticles-based antimicrobial photodynamic therapy

Pourhajibagher

Bahador

2021

BMC Microbiol

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The aim of this study was to evaluate the anti-biofilm and anti-metabolic activities of zeolite-zinc oxide nanoparticles (Zeo/ZnONPs)-based antimicrobial photodynamic therapy (aPDT) against pre-formed polymicrobial biofilms on the orthodontic brackets, as well as, assess the remineralization efficacy on polymicrobial biofilms induced enamel lesions. Following synthesis and characterization of Zeo/ZnONPs, cell cytotoxicity, hemolytic effect, and intracellular reactive oxygen species (ROS) production were determined. The anti-biofilm and anti-metabolic activities of aPDT using different concentrations of Zeo/ZnONPs were investigated. Microhardness tester and DIAGNOdent Pen were used to evaluate the changes of remineralization degree on the treated enamel slabs duration 1 and 3 months. No significant cytotoxicity and erythrocyte hemolysis were observed in treated cells with Zeo/ZnONPs. When irradiated, suggesting that the Zeo/ZnONPs were photoactivated, generating ROS and leading to reduce dose-dependently the cell viability and metabolic activity of polymicrobial biofilms. Also, the enamel surface microhardness value of exposed enamel showed a steady increase with the concentration of Zeo/ZnONPs. No statistically significant differences were shown between aPDT and sodium fluoride varnish as the control group. Overall, Zeo/ZnONPs-based aPDT with the greatest remineralization efficacy of enamel surface can be used as an anti-biofilm therapeutic method, which is involved with their potent ability to produce ROS.

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“…Recently, aPDT has been introduced as an alternative therapy for preventing and treating dental caries via control the formation of the microbial bio lm and controlling the incidence of microbial pathogens without the development of resistance [29][30][31] . Several variables such as the type and concentration of photosensitizers, light sources, irradiation parameters, and physiologic condition of the microorganisms studied must be considered for the effectiveness of aPDT 32 .…”

Section: Discussionmentioning

confidence: 99%

Enhanced Reduction of Polymicrobial Biofilms on the Orthodontic Brackets and Enamel Surface Remineralization Using Zeolite-Zinc Oxide Nanoparticles-Based Antimicrobial Photodynamic Therapy

Pourhajibagher

Bahador

2021

Preprint

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The aim of this study was to evaluate the anti-biofilm and anti-metabolic activities of zeolite-zinc oxide nanoparticles (Zeo\ZnONPs)-based antimicrobial photodynamic therapy (aPDT) against pre-formed polymicrobial biofilms on the orthodontic brackets, as well as, assess the remineralization efficacy on polymicrobial biofilms induced enamel lesions. Following synthesis and characterization of Zeo\ZnONPs, cell cytotoxicity, hemolytic effect, and intracellular reactive oxygen species (ROS) production were determined. The anti-biofilm and anti-metabolic activities of aPDT using different concentrations of Zeo\ZnONPs were investigated. Microhardness tester and DIAGNOdent Pen were used to evaluate the changes of remineralization degree on the treated enamel slabs duration one and three months. No significant cytotoxicity and erythrocyte hemolysis were observed in treated cells with Ze\ZnONPs. When irradiated, suggesting that the Ze\ZnONPs were photoactivated, generating ROS and leading to reduce dose-dependently the cell viability and metabolic activity of polymicrobial biofilms. Also, the enamel surface microhardness value of exposed enamel showed a steady increase with the concentration of Zeo\ZnONPs. No statistically significant differences were shown between aPDT and sodium fluoride varnish as the control group. Overall, Zeo\ZnONPs-based aPDT with the greatest remineralization efficacy of enamel surface can be used as an anti-biofilm therapeutic method, which is involved with their potent ability to produce ROS.

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Efficacy of antimicrobial photodynamic therapy with Rose Bengal and blue light against cariogenic bacteria

Cited by 43 publications

References 36 publications

Antibacterial Activity of Pharmaceutical-Grade Rose Bengal: An Application of a Synthetic Dye in Antibacterial Therapies

Antibacterial Activity of Pharmaceutical-Grade Rose Bengal: An Application of a Synthetic Dye in Antibacterial Therapies

Enhanced reduction of polymicrobial biofilms on the orthodontic brackets and enamel surface remineralization using zeolite-zinc oxide nanoparticles-based antimicrobial photodynamic therapy

Enhanced Reduction of Polymicrobial Biofilms on the Orthodontic Brackets and Enamel Surface Remineralization Using Zeolite-Zinc Oxide Nanoparticles-Based Antimicrobial Photodynamic Therapy

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