Efficacy of antioxidant in the therapy of subclinical mastitis in buffaloes

Kala, Salikineedi Rupa; Rani, N. Lakshmi; Rao, Velamala Vaikunta; Subramanyam, K.V.

doi:10.56825/bufbu.2022.4112661

Cited by 5 publications

(4 citation statements)

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“…A high prevalence of E. coli was detected in right hind (9.57%) and left-fore quarter (8.24%) followed by left hind (8.16%) and right-fore quarter (6.31%). This finding is in agreement with that of [25] who reported a high prevalence in hind quarter, in contrast, [41,42] reported a high prevalence in four quarters. The high prevalence in hind quarters might presumably be associated with increased chance of hind quarters being soiled with urine and faces or by the tail leading to poor udder management [43].…”

Section: Discussionsupporting

confidence: 92%

Isolation and Antibiogram of Escherichia coli Isolated from Selected Dairy Farm at Sebeta, Oromia, Ethiopia

2022

austinjvetscianimhusb

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Mastitis is the most prevalent disease of dairy animals, imparting huge economic losses to the dairy industry. Escherichia coli (E. coli) bacteria are among the common causes of mastitis in dairy animals. A cross-sectional study was carried out from March 2021 to August 2021 on lactating Cattle suffering from mastitis cases to isolate Escherichia coli and to assess their antimicrobial susceptibility pattern in Sebeta town, Oromia Special Zones, Ethiopia. Prevalence of mastitis by California Mastitis Test at cow level was found to be 87.14% (122/140), out of which 12.85% (18/140) and 74.28% (104/140) were clinical and subclinical, respectively. The study revealed that out of the 560 quarters examined, 24 (4.2%) of them were blind and 536 were functional. The Escherichia coli isolated from CMT positive was 8.07% (31/384); from this clinically and sub clinically affected udder were 1.04% (4/384) and 7.03% (27/384) respectively. Finally, the antimicrobial profiles of the 31 E. coli confirmed isolates were assessed using 10 different antimicrobials. Out of the 31 isolates tested, 7 (22.58%) isolates were found to be highly resistant to Ampicillin, 5 (16.12%) isolates were resistant to Trimethoprim+Sulphamethoxazole, and 3 (9.67%) and 4(12.9%) isolates were developed resistant to Amoxicillin+Cluvanate acid and Tetracycline respectively. However, all thirty-one isolates of Escherichia coli were found to be highly susceptible to five antimicrobials namely Ciprofloxacin (100%), Cefoxitin (100%), Meropenem (100%), Cefotaxime (100%) and Gentamycin (100%). In conclusion, this study determined the importance of Escherichia coli as one of mastitis causing bacterium of the dairy industry and investigated its antimicrobial resistance pattern. Thus, researchers would like to emphasize the need for urgent intervention to control the diseases and prevent the associated loss.

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Section: Discussionsupporting

confidence: 92%

Isolation and Antibiogram of Escherichia coli Isolated from Selected Dairy Farm at Sebeta, Oromia, Ethiopia

2022

austinjvetscianimhusb

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“…The high percent of this screening test percent interfere with high concentration somatic cell ratio in milk. current finding were similar to [24,25]. Tab1e 3.…”

Section: Screening Testssupporting

confidence: 91%

Evaluation Effects of Coliform Mastitis on Hematological and Biochemical Parameters in Dairy Cows, in Kirkuk City, Iraq

Al-Bayati

2023

IOP Conf. Ser.: Earth Environ. Sci.

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This study conducted to investigate sub clinical mastitis caused by some coliform pathogens as a common causes mastitis in the cows and to detect some hematological & biochemical parameters, for this purpose 100 milk samples and100 blood samples were collected from apparently cows brought to the veterinary hospital & from different heifer’s in Kirkuk governate, Iraq. our results showed that the incidence of coliform mastitis by using different diagnostic methods. the incidence mastitis to CMT according of management & hygiene milk of processing recorded 35 (42.6 %), 47 ( 57.3%) respectively., However the bacteriologically examination depending on isolation, biochemical manually and vitek system identification. revealed, the incidence as 82.9% positive for bacterial identification reveled that E.coli was predominant ( 33.8 %) bacteria Followed by K. pneumonia and. Proteus mirabilis (11.7 %) &(10.2)% respectively. further hematological parameters we noted that showed anemia with significant decrease (p<0.05) in Hemoglobin, Total red blood cell count, Paced cell volume, lymphocyte and slightly decrease in, while record increase in granulocyte and Wight blood cell count. On the other hand the biochemical profile we showed significant increase in ALT, AST enzymes, While decrease significant(p<0.05) in ALP, Ca, Cl, P, Mg & zn. We conclude from this study, The prevalence of pathogenic bacteria in the milk resulted in a significant alteration in the hamato-biochemical profile in the current study reveled significant monitors for Bovine mastitis and estimate the efficiency and quality of milk consumption.

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“…A lower prevalence of S. aureus was reported by Salem-Bekhit et al (2010) (16.1%) and Amin et al (2011) (14.9%). On the contrary, Ali et al (2015) obtained a higher incidence of S. aureus (60%) recovered from subclinical mastitis in buffalo in India. Moreover, the prevalence of E. coli in our study was nearly similar incidence (30%) observed by Kumar (2009), whereas a comparatively Low frequency of E. coli was documented by Vásquez-García et al (2017) in dairy buffalo.…”

Section: Resultsmentioning

confidence: 85%

Evaluation of Zinc Oxide Nano particles and Curcumin effect on some virulence genes of Staphylococcus aureus and Candida albicans causing subclinical mastitis in buffalo

El-Hamaky

Mohamed²,

El-Khalek³

et al. 2023

Egyptian Journal of Agricultural Research

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Nowadays, there are progressive advancements in the biomedical employment of nanotechnology in human and animal health. Today, the dangers of infectious animal diseases and the continuous application of traditional antibiotics resulted in the elevation of the activities of the drugs resistant genes and the failure of their control. Hence, it is necessary to prevent fungal and bacterial pathogens pollution and their toxicity in dairy animal products. Sixty samples were collected from dairy buffalo farms suffering from subclinical mastitis (20 Samples of each from subclinical mastitis milk, animal feeds, and bedding). The species of Staphylococcus is considered the most predominant species, and Staphylococcus aureus was the most prevalent bacterial isolate. While, yeast, especially Candida albicans, was the most dominant fungal isolate from different samples of dairy buffalo farms. Our antibiogram of S. aureus and Candida albicans showed a high resistance rate against several traditional antimicrobial agents. Therefore, searching for effective drug materials to overcome drug resistance is needed. Herein, ZnONPs and Curcumin are evaluated singly and, in a composite, to inhibit the growth of C. albicans and S. aureus. The inhibitory dose of ZnONPs for C. albicans and S. aureus were (600 and 600 μg /ml) and in Curcumin were (2% and 3%), respectively. However, the hybrid nanocomposite of ZnONPs (100 μg/ml) with Curcumin (0.25% and 1%) caused suppression of the growth of these pathogens. The nanocomposite of low levels of ZnONPs (100 μg /ml) with (0.25% curcumin) significantly increased the inhibitory effects against C. albicans and S. aureus. The molecular study to detect the efficiency of the treatment doses indicated that the exposure of C. albicans and S. aureus to a low dose of ZnONPs (100 µg/ml) did not affect the DNA expression of virulent genes. While the high dose (600 µg/ml) prevented the DNA expression. However, in the Curcumin treatments of isolates with low doses (0.25), the genes' DNA expression was not affected. While, the high dose (1%), removed the DNA band. All inhibition and removal of virulent genes' DNA of pathogens can be obtained by the combination of low levels of ZnONPs and Curcumin. Hence, it is concluded that the nanocomposites of ZnONPs and Curcumin caused significant effects against pathogens.

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Efficacy of antioxidant in the therapy of subclinical mastitis in buffaloes

Cited by 5 publications

References 5 publications

Isolation and Antibiogram of Escherichia coli Isolated from Selected Dairy Farm at Sebeta, Oromia, Ethiopia

Isolation and Antibiogram of Escherichia coli Isolated from Selected Dairy Farm at Sebeta, Oromia, Ethiopia

Evaluation Effects of Coliform Mastitis on Hematological and Biochemical Parameters in Dairy Cows, in Kirkuk City, Iraq

Evaluation of Zinc Oxide Nano particles and Curcumin effect on some virulence genes of Staphylococcus aureus and Candida albicans causing subclinical mastitis in buffalo

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