Efficacy of Beauveria Bassiana and Bacillus Thuringiensis Against Maize Stem Borer Chilo Partellus (Swinhoe) (Lepidoptera: Pyralidae)

Sufyan, Muhammad; Abbasi, Asim; Wakil, Waqas; Gogi, Muhammad Dildar; Arshad, Muhammad; Nawaz, Ahmad; Shabbir, Zeeshan

doi:10.1007/s10343-019-00465-7

Cited by 15 publications

(3 citation statements)

References 41 publications

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“…The eleven virulent isolates of the EPF B. bassiana, M. anisopliae and V. lecanii were collected and maintained in the PTUK laboratories during 2021-22. EPF isolates were sub-cultured and incubated by a single spore method (Sufyan et al, 2019) for 7-10 days at 28±2C on PDA media (Fig 1 A-C.). A pathogenicity test was carried out after the 6-7 subcultures to maintain the virulance of entomopathogen isolates.…”

Section: Sources and Preparation Of Fungal Isolatesmentioning

confidence: 99%

Efficacy of Three Entomopathogenic Fungi Beauveria bassiana, Metarhizium anisopliae and Lecanicillium lecanii Isolates against Black Bean Aphid, Aphis fabae (Scop.) (Hemiptera: Aphididae) on Faba bean (Vicia faba L.)

Qubbaj

Samara

2022

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Background: A laboratory bioassay study was conducted to evaluate the in vitro pathogenicity of different isolates of B. bassiana, M. anisopliae and L. lecanii, against the adults of black bean aphid. Methods: The PCR-based method was used to identify the different isolates molecularly using sequence information from the ITS region. The total genomic DNA of the 19 fungal isolates was recovered from aphid cadavers using CTAB. The amplified DNA using QRT-PCR showed no significant differences in the ANOVA that tested mean cycle threshold (CT) values from the control. Post-molecular identification of the isolated entomopathogen was approved. The single discriminative concentration bioassay was carried out to determine LT50 values for each of twelve isolates to determine the most virulent for further studies. Result: LT50 values for B. bassiana, M. anisopliae and V. lecanii isolates varied from 110-113, 71-75 and 64-77 h, respectively. B. bassiana isolate BBK2, M. anisopliae isolate MAA2 and V. lecanii isolates VLJ2 were selected for further experiments based on their discriminating concentration values. LC50 of BBA post-exposure to isolates of V. lecanii, M. anisopliae and B. bassiana was 46, 269 and 251 ppm, respectively. A significant difference in cumulative mortality was recorded between the three EPF. M. anisopliae showed a higher significant cumulative mortality during the first and second days post-application. Then V. lecanii recorded higher significant cumulative mortality from the third until the seventh-day post-application. V. lecanii showed higher virulence among the other entomopathogenic isolates.

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Section: Sources and Preparation Of Fungal Isolatesmentioning

confidence: 99%

Efficacy of Three Entomopathogenic Fungi Beauveria bassiana, Metarhizium anisopliae and Lecanicillium lecanii Isolates against Black Bean Aphid, Aphis fabae (Scop.) (Hemiptera: Aphididae) on Faba bean (Vicia faba L.)

Qubbaj

Samara

2022

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“…Of all the biopesticides investigated, M. anisopliae is the most extensively researched, and its insecticidal properties have been widely investigated in recent studies [23][24][25]. Additionally, its effectiveness in controlling agricultural insect pests has made it a popular BCA alongside the microbial insecticide Bacillus thuringiensis Berliner (Bacillales: Bacillaceae) [26]. B. bassiana, which was originally isolated from silkworm cadavers by Agostino Bassi in the 19th century, exhibits a broad host range as M. anisopliae, infecting more than 200 insect species across six orders and fifteen families [27,28], inhabiting diverse ecosystems such as stored product insects [29,30], bees [31,32], moths [33][34][35][36] and mosquitos [37][38][39] among others [40,41], making it a highly versatile and effective biopesticide.…”

Section: Introductionmentioning

confidence: 99%

Interactions between Entomopathogenic Fungi and Insects and Prospects with Glycans

Liu

Smagghe

Liu

2023

JoF

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Concerns regarding the ecological and health risks posed by synthetic insecticides have instigated the exploration of alternative methods for controlling insects, such as entomopathogenic fungi (EPF) as biocontrol agents. Therefore, this review discusses their use as a potential alternative to chemical insecticides and especially focuses on the two major ones, Beauveria bassiana and Metarhizium anisopliae, as examples. First, this review exemplifies how B. bassiana- and M. anisopliae-based biopesticides are used in the world. Then, we discuss the mechanism of action by which EPF interacts with insects, focusing on the penetration of the cuticle and the subsequent death of the host. The interactions between EPF and the insect microbiome, as well as the enhancement of the insect immune response, are also summarized. Finally, this review presents recent research that N-glycans may play a role in eliciting an immune response in insects, resulting in the increased expression of immune-related genes and smaller peritrophic matrix pores, reducing insect midgut permeability. Overall, this paper provides an overview of the EPF in insect control and highlights the latest developments relating to the interaction between fungi and insect immunity.

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“…thuringiensis was initially isolated from dead insects and from soil, but it has also been found in phyllosphere and as endophyte of plants (14,15). However, research efforts are mostly focused on the saprophytic lifestyle of B. thuringiensis in insects or on in vitro cultivation in shaken flasks or reactors for biological control of pests (16)(17)(18). In fact, B. thuringiensis and other species from the Bacillus cereus group have been widely used as model for the study of the intricate control of virulence, necrotrophism and sporulation (10,(19)(20)(21).…”

Section: Introductionmentioning

confidence: 99%

The quorum sensing system NprR-NprRB contributes to spreading and fitness in colony biofilms ofBacillus thuringiensis

Verdugo-Fuentes

Rocha

2021

Preprint

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Quorum sensing (QS) are intercellular communication mechanisms to coordinate bacterial gene expression in response to signaling molecules. In Bacillus thuringiensis the QS system NprR-NprRB (receptor protein-signaling peptide) regulates the expression of genes related to nutrient scavenging during necrotrophism and also modulates sporulation onset. However, the relevance of QS in free-living stages of B. thuringiensis is less known. In this work, we depict the contribution of this QS system to spreading in colony biofilms. Through a spreading assay in spotted colonies of B. thuringiensis Bt8741 Wt and derived mutants, we find that the spreading phenotype depends on the NprR regulator and on the extracellular signaling NprRB peptide. We also show that this phenotype is associated to an increased fitness of the bacterium in these experimental conditions. Exogenous addition of a lipopeptide surfactant was sufficient to recover spreading in the ΔnprR-nprRB mutant, indicating that the phenotype could be mediated by the lipopeptide kurstakin. Finally, we suggest that the spreading is relevant in nature, since it occurs in the sole presence of soil nutrients, and it is conserved in several species of Bacillus commonly found in soil. This novel function of NprR-NprRB highlights the relevance of this QS system on the evolution and on the free-lifestyle ecology of B. thuringiensis.

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Efficacy of Beauveria Bassiana and Bacillus Thuringiensis Against Maize Stem Borer Chilo Partellus (Swinhoe) (Lepidoptera: Pyralidae)

Cited by 15 publications

References 41 publications

Efficacy of Three Entomopathogenic Fungi Beauveria bassiana, Metarhizium anisopliae and Lecanicillium lecanii Isolates against Black Bean Aphid, Aphis fabae (Scop.) (Hemiptera: Aphididae) on Faba bean (Vicia faba L.)

Efficacy of Three Entomopathogenic Fungi Beauveria bassiana, Metarhizium anisopliae and Lecanicillium lecanii Isolates against Black Bean Aphid, Aphis fabae (Scop.) (Hemiptera: Aphididae) on Faba bean (Vicia faba L.)

Interactions between Entomopathogenic Fungi and Insects and Prospects with Glycans

The quorum sensing system NprR-NprRB contributes to spreading and fitness in colony biofilms ofBacillus thuringiensis

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