Efficacy of decoquinate against drug sensitive laboratory strains of Eimeria tenella and field isolates of Eimeria spp. in broiler chickens in China

Guo, Fangshen; Suo, Xun; Zhang, G.Z.; Shen, Jianzhong

doi:10.1016/j.vetpar.2007.04.002

Cited by 20 publications

(12 citation statements)

References 17 publications

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“…were consistent with findings in chickens experimentally infected with coccidia (Guo et al 2007;Naidoo et al 2008;Nweze and Obiwulu 2009). Anaemia due to intestinal destruction and resulting blood loss is a consistent finding in Eimeria species infections in chickens (McDougald and Reid 1997).…”

Section: Discussionsupporting

confidence: 89%

“…In Nigeria, commonly used coccidiostats include sulphaquinoxaline and pyrimidine derivatives such as amprolium (Oladoja and Olusanya 2007). However, the toxic effects of these chemicals on poultry (Singh and Gupta 2003;Calo et al 2005), the development of resistance to it by target parasites (Fanatico 2006;Guo et al 2007) and the problem of drug residues in poultry meat (Ogbe et al 2008) suggest herbal remedies as a reasonable alternative.…”

Section: Introductionmentioning

confidence: 99%

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Anticoccidial activity of the methanolic extract of Musa paradisiaca root in chickens

Anosa

Okoro

2010

Trop Anim Health Prod

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The study was designed to evaluate the anticoccidial activity of the methanolic extract of Musa paradisiaca root in chickens. The chickens were divided into six groups of 12 chickens each. Each chicken in five groups was infected with 8,000 infective coccidia (Eimeria tenella) oocysts at day 28 of age while one group served as uninfected control. At day 7 post-infection, two chickens remaining in each group were sacrificed for postmortem examination to confirm coccidiosis. Also at day 7 post-infection, each chicken in four infected groups was given graded doses (250, 500 and 1,000 mg/kg b.w.) of the extract or amprolium (conventional drug). Two groups (an infected and uninfected group) did not receive treatment. Parameters used to assess progress of infection and response to treatment included clinical signs typical of coccidiosis, oocyst count per gramme of faeces (OPG) and packed cell volume (PCV). Treatment of previously infected chickens with M. paradisiaca root extract resulted in a progressive decrease in severity of observed clinical signs, marked reductions in OPG and a gradual increase in PCV. In each case, the changes were dose dependent. There was no significant difference in mean OPG and mean PCV of the extract (at 1,000 mg/kg b.w.) and amprolium-treated groups at termination of the study (at day 50 of age). In the acute toxicity study, the extract was found to be non-toxic to the chickens even at the highest dose of 4,000 mg/kg b.w. The results of this study demonstrated that the extract has anticoccidial activity in a dose-dependent manner and at a dosage of 1,000 mg/kg b.w. had similar efficacy with amprolium in the treatment of chicken coccidiosis.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Anticoccidial activity of the methanolic extract of Musa paradisiaca root in chickens

Anosa

Okoro

2010

Trop Anim Health Prod

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“…Maduramycin is thought to kill coccidium by interrupting their normal intracellular ion balance and influence of Na + -K + -ATPase activity [14]. Decoquinate interferes with the electron transport in the mitochondrial cytochrome system and CytB is an important part of the system [15]. The working mechanism of diclazuril is still unknown.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and drug sensitivity studies on Eimeria tenella field isolates from Hubei Province of China

Tan

Yang

et al. 2017

Parasites Vectors

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BackgroundAvian coccidiosis is an intracellular intestinal parasitic disease, caused by intracellular intestinal parasites from the genus Eimeria, among which Eimeria tenella is one of the most pathogenic species and causes great economic losses. Frequent applications of anticoccidial drugs have resulted in the development of drug-resistance in E. tenella. In the present study, we sought to determine the genetic diversity of E. tenella isolates prevalent in chicken farms in Hubei Province of China and examine their sensitivity to three anticoccidial drugs. The results provide useful information for the prevention and control of coccidiosis in this region.Methods Eimeria tenella oocysts were isolated from faecal samples collected from different commercial broiler production farms in Hubei Province, China. After oocyst sporulation and animal inoculation for expansion of the field isolates, DNA and RNA were extracted from excysted sporozoites for molecular characterization. Species identification of field isolates were performed by polymerase chain reaction (PCR) amplification of the internal transcribed spacer 1 (ITS1) region of ribosomal DNA. Random amplified polymorphic DNA (RAPD) was used for population genetic analysis. Subsequently, sequences of the major sporozoite surface antigen (SAG), micronemal protein 2 (MIC-2) and cytochrome b (cytb) genes from genomic DNA, and the Eimeria tenella cation-transport ATPase (EtCat ATPase) gene from cDNA were obtained for genotyping using multi-sequence alignments. Finally, sensitivity of the field isolates to three commonly used anticoccidial drugs (diclazuril, decoquinate and maduramycin) were tested to assess the prevalence of drug resistance in E. tenella in Hubei Province of China.ResultsAnalysis of the ITS1 sequences indicated that all the isolates were E. tenella. RAPD analysis and multi-sequence alignments of the SAG, MIC-2, EtCat ATPase and cytb showed genetic diversity among these isolates. Finally, drug sensitivity tests demonstrated that all field isolates were sensitive to diclazuril but resistant to decoquinate (except for the isolates from eastern Hubei) and maduramicin.ConclusionsPopulation genetic analysis indicated that genetic polymorphisms among field isolates were closely related with their regional distributions. Drug sensitivity testing demonstrated that E. tenella isolates in Hubei Province were sensitive to diclazuril, but resistant to maduramycin and decoquinate. The results presented here provide important information for the control and preventions of coccidiosis in the Hubei Province of China.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-017-2067-y) contains supplementary material, which is available to authorized users.

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“…All Eimeria species tend to develop drug resistance to most anticoccidial drugs. Consequently, the search for new drugs is always welcomed (Guo et al 2007;Fei et al 2013). Quinfamide, a dichloroacetyl derivative of quinolinol, is widely used to treat intestinal Entamoeba infections in humans at a dose of 300 mg/adult (Padilla et al 1995;Padilla et al 1998;Romero et al 2005).…”

Section: Introductionmentioning

confidence: 99%

In vivo anticoccidial activity of quinfamide in broilers: a preliminary report

Aquino

Alcalá

Gutiérrez

et al. 2016

Italian Journal of Animal Science

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The aim of this trial was to evaluate the anticoccidial efficacy of quinfamide in broilers. Three different treatments were implemented over a 49-day period as follows: quinfamide; quinfamide plus carbopol and decoquinate, all prepared as small pellets and mixed with feed at a final dose of either active principle of 30 ppm. Parameters measured were: weight gain, number of oocysts shedding per gram of litter and degree of gross lesions caused by coccidia. Body weight gain was statistically greater for quinfamide and quinfamide-carbopol groups in comparison to other groups. However, only the quinfamide-carbopol group showed similar efficacy in the occyst counts as compared to the decoquinate group. Statistically significant differences were observed when intestinal lesions score were compared and the less affected group was quinfamide-carbopol. Based on these results, it is concluded that quinfamide possesses a low anticoccidial efficacy. However, this is noticeably improved when it is prepared as pellets with carbopol. The adhesion of carbopol to intestinal mucosa may influence residence time of quinfamide in the gastrointestinal tract, thus enhancing efficacy. ARTICLE HISTORY

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Efficacy of decoquinate against drug sensitive laboratory strains of Eimeria tenella and field isolates of Eimeria spp. in broiler chickens in China

Cited by 20 publications

References 17 publications

Anticoccidial activity of the methanolic extract of Musa paradisiaca root in chickens

Anticoccidial activity of the methanolic extract of Musa paradisiaca root in chickens

Genetic diversity and drug sensitivity studies on Eimeria tenella field isolates from Hubei Province of China

In vivo anticoccidial activity of quinfamide in broilers: a preliminary report

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