The study was conducted in the Department of Plant Protection - College of Agriculture - University of Anbar for the year 2022, as the results of collecting samples from the regions of Anbar and Waist governorates showed the spread of okra root rot disease in most of the study areas, and the results of phenotypic and molecular diagnosis showed the presence of the fungal species Pythium Aphanidermatum, Fusarium proliferatum, Epicoccum nigrum, Itersonilia perplexans, Fusarium chlamydosporum, Rhizoctonia solani, which were deposited in the International Genbank OQ572440, OQ540926, OQ566939, OQ540927, OQ540928, OQ540929 (NCBI) seeds, respectively. Okra, as the tested isolates achieved a significant decrease in the germination of okra seeds on the culture medium (W.A. Water agar) compared with the control treatment that was not contaminated with any of the tested isolates, in which the infection rate was 0.0%.