Efficacy of Enrofloxacin for the Treatment of Salmonids with Bacterial Kidney Disease, Caused by<i>Renibacterium salmoninarum</i>

Hsu, Hui‐Min; Wooster, Gregory A.; Bowser, Paul R.

doi:10.1577/1548-8667(1994)006<0220:eoeftt>2.3.co;2

Cited by 30 publications

(23 citation statements)

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“…The antibiogram performed on 12 R. salmoninarum representative isolates showed that results for Michigan-origin isolates also were similar to previous reports [30][31][32][33]. However, our results of isolate resistances to erythromycin and azithromycin (Oden BNT-BS-02 and Oden BNT-BS1-02), were unexpected since the ''wild-type'' R. salmoninarum strains are known to be sensitive to macrolide antibiotics [30].…”

Section: Discussionsupporting

confidence: 89%

Recovery of Renibacterium salmoninarum from naturally infected salmonine stocks in Michigan using a modified culture protocol

Faisal

Eissa

Starliper

2010

Journal of Advanced Research

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Renibacterium salmoninarum, the causative agent of bacterial kidney disease (BKD), is a fastidious and slow-growing bacterium that is extremely difficult to grow in vitro. Herein, we describe a modified primary culture protocol that encompasses a modified bacteriological culture medium and a tissue processing procedure. In order to facilitate the release of R. salmoninarum from granulomatous tissues, kidneys of infected fish were homogenized in a high speed stomacher. The kidney disease medium (KDM2), routinely used for primary culture of R. salmoninarum was modified by the addition of antibiotics and metabolites. When a relatively large inoculum of diluted kidney homogenate was streak-plate inoculated onto the modified KDM2, colonial growth of R. salmoninarum was achieved within 5-7 days, compared to the standard of two weeks or more. The modified procedure was then used to determine the prevalence of R. salmoninarum among representative captive and feral salmonid stocks in Michigan. Prevalence and clinical manifestations varied among species, strains of fish, and locations; however, R. salmoninarum isolates were biochemically homogenous. The improved primary culture procedure described in this study enabled selective and quick isolation of R. salmoninarum. Also, the isolates retrieved in this study constitute a unique biological resource for future studies of R. salmoninarum in the Laurentian Great Lakes.

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Section: Discussionsupporting

confidence: 89%

Recovery of Renibacterium salmoninarum from naturally infected salmonine stocks in Michigan using a modified culture protocol

Faisal

Eissa

Starliper

2010

Journal of Advanced Research

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“…Findings of this study also demonstrated that the cultural, morphological and biochemical characteristics of lake whitefish R. salmoniarum isolates are uniform and coincide with those previously reported from Michigan and worldwide (Holt et al, 2000;Eissa, 2005). In the same context, the antibiograms performed on 12 representative lake whitefish R. salmoninarum isolates showed that they were also similar to each other and to other strains isolated worldwide (Austin, 1985;Hsu et al, 1994). Moreover, our identifications were further confirmed by nPCR results, as all lake whitefish R. salmoninarum isolates exhibited the 320 bp band characteristic for R. salmoninarum.…”

Section: Discussionsupporting

confidence: 74%

Assessment of Renibacterium salmoninarum infections in four lake whitefish (Coregonus clupeaformis) stocks from northern Lakes Huron and Michigan

Faisal

Loch

Brenden

et al. 2010

Journal of Great Lakes Research

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“…It possesses a broad spectrum of activity against most gram‐negative organisms and some gram‐positive bacteria, as well as mycoplasmas. It has been demonstrated to be efficacious against common bacterial pathogens including Aeromonas salmonicida, Aeromonas hydrophila, Vibrio anguillarum, Yersinia spp and Renibacterium salmoninarum (Akhlaghi & Sharifi Yazdi, ; Hsu, Wooster, & Bowser, ; Maluping et al., ). China has given permission for ENR to be used in aquaculture, and it has become an essential strategy to confront and alleviate infectious disease and reduce animal mortality.…”

Section: Pharmacokinetic Parameters Of Enrofloxacin and Ciprofloxacinmentioning

confidence: 99%

Pharmacokinetics of enrofloxacin after oral, intramuscular and bath administration in crucian carp (Carassius auratus gibelio)

Fan

Wang

et al. 2017

Vet Pharm & Therapeutics

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The pharmacokinetics of enrofloxacin (ENR) was studied in crucian carp (Carassius auratus gibelio) after single administration by intramuscular (IM) injection and oral gavage (PO) at a dose of 10 mg/kg body weight and by 5 mg/L bath for 5 hr at 25°C. The plasma concentrations of ENR and ciprofloxacin (CIP) were determined by HPLC. Pharmacokinetic parameters were calculated based on mean ENR or CIP concentrations using WinNonlin 6.1 software. After IM, PO and bath administration, the maximum plasma concentration (Cmax) of 2.29, 3.24 and 0.36 μg/ml was obtained at 4.08, 0.68 and 0 hr, respectively; the elimination half‐life (T1/2β) was 80.95, 62.17 and 61.15 hr, respectively; the area under the concentration–time curve (AUC) values were 223.46, 162.72 and 14.91 μg hr/ml, respectively. CIP, an active metabolite of enrofloxacin, was detected and measured after all methods of drug administration except bath. It is possible and practical to obtain therapeutic blood concentrations of enrofloxacin in the crucian carp using IM, PO and bath immersion administration.

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Efficacy of Enrofloxacin for the Treatment of Salmonids with Bacterial Kidney Disease, Caused byRenibacterium salmoninarum

Cited by 30 publications

References 0 publications

Recovery of Renibacterium salmoninarum from naturally infected salmonine stocks in Michigan using a modified culture protocol

Recovery of Renibacterium salmoninarum from naturally infected salmonine stocks in Michigan using a modified culture protocol

Assessment of Renibacterium salmoninarum infections in four lake whitefish (Coregonus clupeaformis) stocks from northern Lakes Huron and Michigan

Pharmacokinetics of enrofloxacin after oral, intramuscular and bath administration in crucian carp (Carassius auratus gibelio)

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