Efficacy of Hl-a Matching in Eurotransplant

Murine Ia and human DR antigens: homology of amino-terminal sequences.

“…Several lines of evidence indicate that the xenoantisera react with the same antigenic structures as do the DR alloantisera. Both react with B lymphoid cells (15,22,23) and are potent inhibitors of mixed lymphocyte reactions (10,11,15,24). Also, incubation of target cells with F(ab)2 fragments of xenoantisera (12,25) (6,7,26) as well as shifts in molecular weights of presumedly HLA-D locus gene products p29 and p34 have been observed previously (20).…”

Immediate Complement Consumption in Human Renal Allografts

“…In recent publications the point has been stressed that identification of responsiveness, as evidenced by cytotoxins present in the patient's serum may be equally or even more important than H L -A typing for selection of kidney transplant recipients [5][6][7]. When leukocyte antibodies are absent, matching for the H L -A antigens has little effect on graft prognosis [7].…”

“…When leukocyte antibodies are absent, matching for the H L -A antigens has little effect on graft prognosis [7]. On the other hand, no single antibody test has unique predictive significance and the retrospective method of determining whether a recipient responds to blood transfusions is not acceptable as a prospective test [5].…”

“…The standard onestage cytotoxicity technique was used for typing, antibody testing, and cross-match ing [7]. Four recipients were mismatched for one antigen, 6 for 2, and 16 patients were mismatched for 3 or 4 antigens.…”

“…The state of immunity of prospective kidney transplant recipients has been identified as an important factor for the outcome of kidney trans plantation [7], Serial determinations of multispecific lymphocytotoxic antibodies have been said to be of predictive value for the identification of potentially responsive and unresponsive recipients [5], If antibodiesdetected or not by standard antibody testing -were present at the time of transplantation one could expect that immediate intrarenal complement activation occurs in patients in the same way as described for the experi mental animal [3, 4J. We have studied immediate intrarenal complement consumption in 26 primary cadaveric kidney transplantations with the following assumptions in mind: (1) in anuric patients complement con sumption could be helpful for differential diagnosis of ischemic kidney damage and accelerated rejection; (2) complement consumption could re flect the actual response of a recipient to his graft, and thus the prognosis of kidney function, and (3) it could serve as a guideline for the intensity of immunosuppressive therapy required to prevent rejection.…”