Efficacy of <i>Megasphaera elsdenii </i>inoculation in subacute ruminal acidosis in cattle

Arik, Huzur Derya; Gülşen, Nurettin; Hayırlı, A.; Alataş, Mustafa Selçuk

doi:10.1111/jpn.13034

Cited by 15 publications

(17 citation statements)

References 26 publications

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“…Furthermore, it has been reported that M. elsdenii utilizes lactate, glucose and maltose thus would compete with LAB for substrate, leading to reducing the lactate concentration and in the meantime enhancing VFA production [ 41 , 64 ]. It is also suggested that the efficacy of M. elsdenii on prevention of SARA might depend on the dietary grain types and the endogenous rumen microbiota, and meanwhile, the supplementation of this bacterial species can alter the rumen microflora by decreasing S. bovis counts and increasing protozoa counts [ 42 ]. However, the past research did not take the individualized rumen microbiome into account on how they respond to this DFM differently.…”

Section: Introductionmentioning

confidence: 99%

“…Usually, dairy ruminants are at high risks during these periods due to the stress from calving, changing diets from forage-based types to high-concentrate diets, and lactation as well as negative energy balance and potential inflammations or metabolic disorders. Some studies have reported that DFMs (mainly combination of LAB, LUB, or yeast) can improve milk production, feed efficiency and health performance in dairy cows [ 40 , 42 ], however, the efficacy was not consistent [ 122 ].…”

Section: Introductionmentioning

confidence: 99%

“…Some studies have reported that DFMs (mainly combination of LAB, LUB, or yeast) can improve milk production, feed efficiency and health performance in dairy cows [ 40 , 42 ], however, the efficacy was not consistent [ 122 ].…”

Section: Introductionmentioning

confidence: 99%

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Implication and challenges of direct-fed microbial supplementation to improve ruminant production and health

Ban

Guan

2021

J Animal Sci Biotechnol

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Direct-fed microbials (DFMs) are feed additives containing live naturally existing microbes that can benefit animals’ health and production performance. Due to the banned or strictly limited prophylactic and growth promoting usage of antibiotics, DFMs have been considered as one of antimicrobial alternatives in livestock industry. Microorganisms used as DFMs for ruminants usually consist of bacteria including lactic acid producing bacteria, lactic acid utilizing bacteria and other bacterial groups, and fungi containing Saccharomyces and Aspergillus. To date, the available DFMs for ruminants have been largely based on their effects on improving the feed efficiency and ruminant productivity through enhancing the rumen function such as stabilizing ruminal pH, promoting ruminal fermentation and feed digestion. Recent research has shown emerging evidence that the DFMs may improve performance and health in young ruminants, however, these positive outcomes were not consistent among studies and the modes of action have not been clearly defined. This review summarizes the DFM studies conducted in ruminants in the last decade, aiming to provide the new knowledge on DFM supplementation strategies for various ruminant production stages, and to identify what are the potential barriers and challenges for current ruminant industry to adopt the DFMs. Overall literature research indicates that DFMs have the potential to mitigate ruminal acidosis, improve immune response and gut health, increase productivity (growth and milk production), and reduce methane emissions or fecal shedding of pathogens. More research is needed to explore the mode of action of specific DFMs in the gut of ruminants, and the optimal supplementation strategies to promote the development and efficiency of DFM products for ruminants.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Implication and challenges of direct-fed microbial supplementation to improve ruminant production and health

Ban

Guan

2021

J Animal Sci Biotechnol

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“…Indeed, when strains of Ruminococcus albus and Ruminococcus flavefaciens, all of which were isolated from the rumen, were inoculated into the rumen of adult sheep, the inoculants failed to persist or improve the intended fiber digestion [10]. Megasphaera elsdenii inoculation to the rumen of Holstein heifers suffering from subacute ruminal acidosis (SARA) did not increase the abundance of M. elsdenii or alleviate the SARA conditions although it altered the rumen VFA profiles [11][12][13]. Inoculation with whole rumen contents from non-milkfat-depressed cows only slightly accelerated the recovery of de novo synthesis of fatty acids in milk fatdepressed cows [14].…”

Section: Introductionmentioning

confidence: 99%

Repeated inoculation with fresh rumen fluid before or during weaning modulates the microbiota composition and co-occurrence of the rumen and colon of lambs

Zhang

Liu

et al. 2020

BMC Microbiol

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Background: Many recent studies have gravitated towards manipulating the gastrointestinal (GI) microbiome of livestock to improve host nutrition and health using dietary interventions. Few studies, however, have evaluated if inoculation with rumen fluid could effectively reprogram the development of GI microbiota. We hypothesized that inoculation with rumen fluid at an early age could modulate the development of GI microbiota because of its low colonization resistance. Results:In this study, we tested the above hypothesis using young lambs as a model. Young lambs were orally inoculated repeatedly (four times before or twice during gradual weaning) with the rumen fluid collected from adult sheep. The oral inoculation did not significantly affect starter intake, growth performance, or ruminal fermentation. Based on sequencing analysis of 16S rRNA gene amplicons, however, the inoculation (both before and during weaning) affected the assemblage of the rumen microbiota, increasing or enabling some bacterial taxa to colonize the rumen. These included operational taxonomic units (OTUs) belonging to Moryella, Acetitomaculum, Tyzzerella 4, Succiniclasticum, Prevotella 1, Lachnospiraceae, Christensenellaceae R-7 group, Family XIII AD3011, and Bacteroidales S24-7 corresponding to inoculation before weaning; and OTUs belonging to Succiniclasticum, Prevotellaceae UCG-003, Erysipelotrichaceae UCG-004, Prevotella 1, Bacteroidales S24-7 gut group uncultured bacterium, and candidate Family XIII AD3011 corresponding to inoculation during weaning. Compared to the inoculation during weaning, the inoculation before weaning resulted in more co-occurrences of OTUs that were exclusively predominant in the inoculum. However, inoculation during weaning appeared to have more impacts on the colonic microbiota than the inoculation before weaning. Considerable successions in the microbial colonization of the GI tracts accompanied the transition from liquid feed to solid feed during weaning.Conclusions: Repeated rumen fluid inoculation during early life can modulate the establishment of the microbiota in both the rumen and the colon and co-occurrence of some bacteria. Oral inoculation with rumen microbiota may be a useful approach to redirect the development of the microbiota in both the rumen and colon.

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“…Furthermore, adverse effects including lowered feed intake and digestion often result at effective doses (Patra and Yu, 2014). Although from a microbiological perspective, rumen microbes of desired features can be inoculated into a rumen to improve certain aspects of the rumen function, such as fiber digestion, inoculation of the rumen of adult ruminants with Ruminococcus albus and R. flavefaciens did not enhance fiber digestion (Krause et al, 2001), while Megasphaera elsdenii inoculation did not attenuate subacute ruminal acidosis (SARA) (Klieve et al, 2003;Zebeli et al, 2012;Arik et al, 2019). Inoculation of dairy cows that exhibited milk fat depression with whole rumen content from non-milk fat-depressed cows only slightly accelerated the recovery of de novo synthesis of fatty acids (FA) in the former (Rico et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Repeated Inoculation of Young Calves With Rumen Microbiota Does Not Significantly Modulate the Rumen Prokaryotic Microbiota Consistently but Decreases Diarrhea

Zhang

et al. 2020

Front. Microbiol.

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The complex rumen microbiota exhibits some degree of host specificity. The undeveloped simple rumen microbiota is hypothetically more amendable. The objective of this study was to investigate if the rumen prokaryotic microbial assemblage of young calves can be reprogrammed by oral inoculation with rumen microbiota of adult cows. Twenty newborn male calves were randomly assigned to four groups (n = 5 per group), with two groups being orally inoculated with rumen microbiota (fresh rumen fluid) collected from two lactating dairy cows, while the other two groups receiving autoclaved rumen fluid collected from another two donor cows. Each calf was orally drenched with 100, 200, 300, 400, and 500 mL of the rumen fluid at d3, d7, d21, d42, and d50, respectively, after birth. The inoculation with rumen microbiota did not affect (P % 0.05) feed intake, average daily gain (ADG), heart girth, or feed conversion ratio but significantly (P $ 0.01) lowered instance of diarrhea. At the age of 77 days (27 days post-weaning), all the calves were slaughtered for the sampling of rumen content and determination of empty rumen weight. Rumen fermentation characteristics were not affected (P % 0.05) by the inoculation. Rumen prokaryotic microbiota analysis using metataxonomics (targeting the V4 region of the 16S rRNA genes) showed that the calf rumen prokaryotic microbiota differed from that of the donors. Two Succinivibrionaceae OTUs, two Prevotella OTUs, and one Succiniclasticum OTU were predominant (relative abundance % 2%) in the donors, but only one Succinivibrionaceae OTU was found in the calves. On the other hand, five other Prevotella OTUs were predominant (%3%) in the calves, but none of them was a major OTU in the donors. No correlation was observed in relative abundance of major OTUs or genera between the donor and the calves. Principal coordinates analysis (PCoA) based on weighted UniFrac distance showed no significant (P % 0.05) difference in the overall rumen prokaryotic microbiota profiles among the four calf groups, and principal component analysis (PCA) based

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Efficacy of Megasphaera elsdenii inoculation in subacute ruminal acidosis in cattle

Cited by 15 publications

References 26 publications

Implication and challenges of direct-fed microbial supplementation to improve ruminant production and health

Implication and challenges of direct-fed microbial supplementation to improve ruminant production and health

Repeated inoculation with fresh rumen fluid before or during weaning modulates the microbiota composition and co-occurrence of the rumen and colon of lambs

Repeated Inoculation of Young Calves With Rumen Microbiota Does Not Significantly Modulate the Rumen Prokaryotic Microbiota Consistently but Decreases Diarrhea

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