Efficacy of Immediate, 24 Hours Versus 48 Hours Culture Method in Cytogenetic Study of Pediatric Acute Lymphoblastic Leukemia

Abstract

Background: Leukemia is the most common childhood cancer in India with relative proportion varying between 25 and 40%. 60 to 85% of all leukemias reported are acute lymphoblastic leukemia (ALL).Karyotyping is a technique that provides critical diagnostic and prognostic information that allows the delivery of appropriate treatment. Success rate depends on sample conditions, type of disease and culture conditions. Aim: To compare efficacy of culture methods at varying times in karyotyping for genetic abnormalities of Acute Lymphoblastic Leukemia. Materials and Methods: Bone marrow aspirates (0.6 ml) after adding 15 ml RPMI medium were divided into three parts for immediate(direct) culture, 24 hour culture and 48 hour culture method , were incubated according to their respective time duration and karyotyping was done. Result: In immediate culture, there were very few small sized cells with no metaphases. In 24 hour culture, cells had a moderate population with no analysable metaphases. In 48 hour culture, cell population was high with bigger sized cells and 20 or more metaphases were observed per slide. Conclusion: 48 hours culture method is better than immediate and 24 hour culture in ALL patients.

Exosomes derived from MSC as drug system in osteoarthritis therapy

“…Currently, MSCs from the 3rd to 6th generation are generally used. When cell confluence reaches 80%–90%, serum-free medium is replaced, and cells are cultured for 24–48 h ( Jha et al, 2016 ). The resulting cell culture supernatant contains not only exosomes but also various components like dead cells, cell fragments, microvesicles, and apoptotic bodies.…”