Introduction:The parafollicular cells or clear (C) cells in man are part of neuroendocrine system under Amine Precursor Uptake and Decarboxylation (APUD) cells. Their role in adults has been reputable but in foetus is still unclear.
Background: Leukemia is the most common childhood cancer in India with relative proportion varying between 25 and 40%. 60 to 85% of all leukemias reported are acute lymphoblastic leukemia (ALL).Karyotyping is a technique that provides critical diagnostic and prognostic information that allows the delivery of appropriate treatment. Success rate depends on sample conditions, type of disease and culture conditions. Aim: To compare efficacy of culture methods at varying times in karyotyping for genetic abnormalities of Acute Lymphoblastic Leukemia. Materials and Methods: Bone marrow aspirates (0.6 ml) after adding 15 ml RPMI medium were divided into three parts for immediate(direct) culture, 24 hour culture and 48 hour culture method , were incubated according to their respective time duration and karyotyping was done. Result: In immediate culture, there were very few small sized cells with no metaphases. In 24 hour culture, cells had a moderate population with no analysable metaphases. In 48 hour culture, cell population was high with bigger sized cells and 20 or more metaphases were observed per slide. Conclusion: 48 hours culture method is better than immediate and 24 hour culture in ALL patients.
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