Efficacy of latex agglutination and quantification methods for determination of C-reactive protein (CRP) in pediatrie sera

Wadsworth, C.; Wadsworth, Elsa

doi:10.1016/0009-8981(84)90138-4

Cited by 16 publications

(12 citation statements)

References 15 publications

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“…Laboratory assessment included complete blood count [Coulter 1660], C-reactive protein [AVITEX- CRP latex test [23]], ESR [Westergren method [24]], serum calcium [colorimetric method [25]], serum alkaline phosphatase [autoanalyzer], serum phosphorus [direct UV method without reduction [26]] and urinary phosphorus [colorimetric methods using an autoanalyzer (Multistat III Plus, Instrumentation Laboratory, Inc, Lexington, MA)]. Colonoscopy and esophago-gastrodudenoscopy [Pentax videosopes, PENYAX GmbH CO. Juulius Vosseler Strasse 104, 22527 Hamburg, Germany] with histopathologic assessment of multiple biopsies were done for all patients.…”

Section: Methodsmentioning

confidence: 99%

Fibroblast growth factor 23 contributes to diminished bone mineral density in childhood inflammatory bowel disease

et al. 2012

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BackgroundDiminished bone mineral density (BMD) is of significant concern in pediatric inflammatory bowel disease (IBD). Exact etiology is debatable. The recognition of fibroblast growth factor 23 (FGF23), a phosphaturic hormone related to tumor necrosis factor alpha (TNF-α) makes it plausible to hypothesize its possible relation to this pathology.MethodsIn this follow up case control study, BMD as well as serum levels of FGF23, calcium, phosphorus, alkaline phosphatase, creatinine, parathyroid hormone, 25 hydroxy vitamin D3 and 1, 25 dihydroxy vitamin D3 were measured in 47 children with IBD during flare and reassessed in the next remission.ResultsLow BMD was frequent during IBD flare (87.2%) with significant improvement after remission (44.7%). During disease flare, only 21.3% of patients had vitamin D deficiency, which was severe in 12.8%. During remission, all patients had normal vitamin D except for two patients with Crohn’s disease (CD) who remained vitamin D deficient. Mean value of serum FGF23 was significantly higher among patients with IBD during flare compared to controls. It showed significant improvement during remission but not to the control values. 1, 25 dihydroxy vitamin D3, FGF23, serum calcium and urinary phosphorus were significant determinants of BMD in IBD patients.ConclusionsWe can conclude that diminished BMD in childhood IBD is a common multifactorial problem. Elevated FGF23 would be a novel addition to the list of factors affecting bone mineral density in this context. Further molecular studies are warranted to display the exact interplay of these factors.

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Section: Methodsmentioning

confidence: 99%

Fibroblast growth factor 23 contributes to diminished bone mineral density in childhood inflammatory bowel disease

et al. 2012

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“…munoassay (PCIA) with a sensitivity of 1 nglml (81), (2) spot immunoprecipitate assay (SIA) with a sensitivity of 1-.25 pg/rnl (82), and (3) immunoradiometric assay (IRMA) with a detection range of from 1 ng/ml to lOpJml (83). Each of these methods has advantages and disadvantages in its application in the clinical laboratory.…”

Section: Source Of Crp and Normal Valuesmentioning

confidence: 99%

C‐Reactive protein: Current status and future perspectives

Hokama

Nakamura

1987

Clinical Laboratory Analysis

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This review encompasses all aspects of re-Reactive protein analysis relative to simplicity cent C-Reactive protein studies. Emphasis has and sensitivity. The responses of C-Reactive been in the areas of (1) the physical, chemical, protein in various diseases and its future prosand potential functions of C-Reactive protein; pects are also discussed in this review. and (2) the pass and recent procedures of C-

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“…The CRP-Latex test is a rapid agglutination procedure for the direct determination of CRP on slides by semi quantitative method [19]. Human CRP antibodies agglutinate in the presence of CRP in patient's serum.…”

Section: Methodsmentioning

confidence: 99%

Assessment of Immunity Status in Patients with Pancreatic Cancer

Geetha

Jeyachristy

Surendran

2006

J. Clin. Biochem. Nutr.

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Summary Wound infection and abscess are the common post operative complications in pancreatic cancer patients. The aim of this study was to evaluate the status of immunity in patients with cancer in the head of pancreas by analyzing the levels of immunoglobulins, compliments, acute phase reactants and the efficiency of neutrophils for phagocytosis. The study was conducted on 89 patients with cancer in the pancreatic acini /pancreatic duct/ head of pancreas/ periampullary region and on equal number of age and sex matched normal healthy volunteers. All the patients were in a need to undergo either pancreaticoduodenectomy or duodenal preserving resection of head of the pancreas. The levels of IgM, IgA, IgE, C3, C4, C-reactive proteins, haptaglobin, α2 macroglobulin were assayed in the blood samples. The level of phagocytosis and bactericidal activity were evaluated in neutrophils. The level of IgM was significantly low and the levels of IgA and IgE were significantly high in pancreatic cancer patients. The levels of C-reactive protein, haptaglobin, α2 macroglobulin , C3 and C4 were elevated significantly. The phagocytic efficiency and the bactericidal action of polymorphonuclear lymphocytes and mononuclear cells were low in pancreatic cancer patients when compared to normal control subjects. In pancreatic cancer patients the antibody mediated immunity is disturbed and the acute phase response is stimulated to counteract the infection and the abnormal metabolites released from malignant cells. The functional impairment of phagocytes might be responsible for the post operative complications in pancreatic cancer patients.

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Efficacy of latex agglutination and quantification methods for determination of C-reactive protein (CRP) in pediatrie sera

Cited by 16 publications

References 15 publications

Fibroblast growth factor 23 contributes to diminished bone mineral density in childhood inflammatory bowel disease

Fibroblast growth factor 23 contributes to diminished bone mineral density in childhood inflammatory bowel disease

C‐Reactive protein: Current status and future perspectives

Assessment of Immunity Status in Patients with Pancreatic Cancer

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