Efficacy of malachite green mediated photodynamic therapy on treatment of Cutaneous Leishmaniasis: In vitro study

Çalışkan, Serçin Özlem; Ilikci-Sagkan, Rahsan; Karakaş, Hatice; Sever, Sevgi; Yıldırım, Cansu; Balikci, Mısra; Ertabaklar, Hatice

doi:10.1016/j.pdpdt.2022.103111

Cited by 7 publications

(5 citation statements)

References 25 publications

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“…In a study by Ozlem-Caliskan et al., it was shown that malachite green (MG) has PS effects in PDT against Leishmania tropica disease. Their findings confirmed that MG at 6.25 μM dose in combination with 46.4 J/cm 2 light at a wavelength of 550 nm exposure blocked cell cycle at G0/G1 phase ( 134 ). In 2019, Qi and colleagues reported the effects of palmatine hydrochloride (PaH) as alkaloid drug-mediated PDT on oral squamous cell carcinoma (OSCC).…”

Section: Cca Following Pdtmentioning

confidence: 59%

“…They concluded that photocyanine mainly arrested the CC at G2/M phase and elevated the apoptosis rate via activation of caspase 3 (131). Another in vitro study showed that asymmetric glycophthalocyanine (GPh3) promotes the efficiency of PDT by arresting CC at G2/M stage; eventually, mitotic cell death happened in HeLa cells (132). Furthermore, it is reported that Zn (II)-phthalocyanine (ZnPc) in…”

Section: Cca At G2/m and M Phasementioning

confidence: 99%

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Photodynamic therapy induced cell cycle arrest and cancer cell synchronization: review

2023

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Cell cycle arrest (CCA) is seen as a prime candidate for effective cancer therapy. This mechanism can help researchers to create new treatments to target cancer cells at particular stages of the cell cycle (CC). The CCA is a characteristic of various therapeutic modalities, including radiation (RT) and chemotherapy (CT), which synchronizes the cells and facilitates the standardization of radio-chemotherapy protocols. Although it was discovered that photodynamic treatment (PDT) had a biological effect on CCA in cancer cells, the mechanism remains unclear. Furthermore, besides conventional forms of cell death such as apoptosis, autophagy, and necrosis, various unconventional types of cell death including pyroptosis, mitotic catastrophe, paraptosis, ferroptosis, necroptosis, and parthanatos after PDT have been reported. Thus, a variety of elements, such as oxygen, the tumor’s microenvironment, the characteristics of light, and photosensitizer (PS), influence the effectiveness of the PDT treatment, which have not yet been studied clearly. This review focuses on CCA induced by PDT for a variety of PSs agents on various cell lines. The CCA by PDT can be viewed as a remarkable effect and instructive for the management of the PDT protocol. Regarding the relationship between the quantity of reactive oxygen species (ROS) and its biological consequences, we have proposed two mathematical models in PDT. Finally, we have gathered recent in vitro and in vivo studies about CCA post-PDT at various stages and made suggestions about how it can standardize, potentiate, and customize the PDT methodology.

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Section: Cca Following Pdtmentioning

confidence: 59%

Section: Cca At G2/m and M Phasementioning

confidence: 99%

Photodynamic therapy induced cell cycle arrest and cancer cell synchronization: review

2023

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“…Macrophages incubated with curcumin and exposed to ultrasound irradiation experience an interaction between activated curcumin and molecular oxygen, producing cytotoxic ROS, especially singlet oxygen, which ultimately leads to cell death [42,[52][53][54][55]. Wang F. et al investigated the sonodynamic effect of curcumin on THP-1-derived macrophages using slightly different parameters than our study [17].…”

Section: Discussionmentioning

confidence: 84%

Synergizing Immune Balance: Curcumin Gold Nanoparticles and Ultrasound Irradiation for Macrophage Down-Regulation

Teixeira,

Gonçalves,

Vieira

et al. 2024

AppliedChem

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The multifaceted health benefits of curcumin (Curcuma longa), attributed to its antioxidant, antitumor, and anti-inflammatory activities, have drawn significant scientific attention. Curcumin shows promise as a potential modulator of macrophage polarization, offering a natural strategy for managing inflammation and promoting tissue repair. However, a limiting factor for this beneficial molecule is its limited bioavailability due to its low solubility in water. This study aimed to quantify the effect of curcumin gold nanoparticle (CurAuNP)-mediated ultrasound irradiation on THP-1-derived macrophages as potential therapeutic targets. The photoreduction method was applied to synthesize the gold nanoparticles with curcumin as a ligand (CurAu). The effect of adding polyethylene glycol in the synthesis process was studied (CurAuPEG). CurAuNP characterization included UV/Vis, Zeta potential, transmission electron microscopy, and FTIR. The amount of singlet oxygen released by curcumin and CurAuNPs was quantified by observing 1.3-diphenylisobenzofuran quenching upon ultrasound irradiation (1 MHz and 1 W/cm2). The results indicated that ultrasound therapy for 4 min with CurAuNPs significantly enhanced singlet oxygen generation and reduced macrophage viability compared to curcumin alone. The increased sonoluminescence and curcumin delivery facilitated by CurAuNPs led to greater curcumin activation. Consequently, CurAuNPs could offer promising therapeutic options for modulating macrophage polarization in pro-inflammatory and anti-inflammatory stages.

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“…Pasado este período de incubación se adicionaron 100 μl/pozo de una solución de 50% de isopropanol (≥ 99.8%, Merck Millipore, Alemania) y 10% de dodecil sulfato de sodio (SDS) (≥ 85.0%, Merck Millipore, Alemania) para solubilizar los cristales de formazán. Los platos se incubaron durante otros 30 minutos y la producción de formazán (que es proporcional al porcentaje de células viables) se midió en un lector de microplacas (Benchmark Bio-Rad Hercules CA, USA) a una densidad óptica de 570 nm (Ozlem-Caliskan et al, 2022).…”

Section: Actividad Citotóxica Sobre Células U-937unclassified

Actividad antiprotozoaria y antioxidante in vitro de extractos y metabolitos mayoritarios aislados de Pilocarpus alvaradoi (Rutaceae)

Marin,

Espinosa,

Guzman

et al. 2024

Inf. tecnol.

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Esta investigación evalua la actividad antiprotozoaria y antioxidante de extractos etanólicos de hojas y corteza de la especie Pilocarpus alvaradoi. La actividad antioxidante se mide usando los métodos DPPH•, ABTS•+ y FRAP. La actividad antiprotozoaria in vitro es medida frente a los parásitos de Leishmania (V.) panamensis, Tripanosoma cruzi y Plasmodium falciparum. La citotoxicidad se examina frente a células U937. Los compuestos se identifican mediante espectroscopia de RMN. Como resultado se identifican tres compuestos: sesamina, escopoletina y bergapteno. Los extractos etanólicos y subextracto en acetato de etilo de corteza muestran actividad antioxidante de 13.5 y 19.5 mg/L (ABTS•+), 17.4 y 21.1 mg/L (DPPH•) y 52.0 y 51.5 mg/L (FRAP) respectivamente. El extracto etanólico de corteza presenta moderada actividad leishmanicida, antiplásmodial y tripanocida con valores de CE50 de 47.90, 28.05 y 49.06 µg/mL, respectivamente. En conclusión, las sustancias obtenidas de P. alvaradoi se reportan por primera vez para esta especie.

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Efficacy of malachite green mediated photodynamic therapy on treatment of Cutaneous Leishmaniasis: In vitro study

Cited by 7 publications

References 25 publications

Photodynamic therapy induced cell cycle arrest and cancer cell synchronization: review

Photodynamic therapy induced cell cycle arrest and cancer cell synchronization: review

Synergizing Immune Balance: Curcumin Gold Nanoparticles and Ultrasound Irradiation for Macrophage Down-Regulation

Actividad antiprotozoaria y antioxidante in vitro de extractos y metabolitos mayoritarios aislados de Pilocarpus alvaradoi (Rutaceae)

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