Efficacy of Mentha pulegium essential oil encapsulated into nanostructured lipid carriers as an in vitro antibacterial and infected wound healing agent

Khezri, Keyvan; Farahpour, Mohammad Reza; Rad, Shokoufeh Mounesi

doi:10.1016/j.colsurfa.2020.124414

Cited by 72 publications

(33 citation statements)

References 34 publications

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“…Other genes involved were TGF-b, and IL-10 whose expressions increased on day 7. IL-10 is a factor that reduces the production of pro-inflammatory cytokine, but TGF-b leads the phase towards the proliferative phase by improving the proliferation and differentiation of fibroblasts, collagen production, and wound contraction (Khezri et al 2020). Seemingly, both genes are of greatly important roles in decreasing the inflammation and passing to the proliferative phase.…”

Section: Discussionmentioning

confidence: 99%

“… 2020 ; Khezri et al. 2020 ). The wound healing process comprises several phases, including coagulation, inflammation, epithelialization, granulation tissue formation, and tissue remodelling (Daemi et al.…”

Section: Introductionmentioning

confidence: 99%

“…The bacteria are commonly detected in the upper and deepest region of wound bed (Serra et al 2015). Colonization of S. aureus and P. aeruginosa in the wound site postpones the wound healing process (Farahpour et al 2020;Khezri et al 2020). The wound healing process comprises several phases, including coagulation, inflammation, epithelialization, granulation tissue formation, and tissue remodelling (Daemi et al 2019a(Daemi et al , 2019bFarahpour et al 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Fibroblast growth factor-2 (FGF-2) is a protein that participates in the wound healing process (Souto et al 2020). reduces the production of pro-inflammatory cytokine, but tumour growth factor-b (TGF-b) has roles in increasing the proliferative phase improving the proliferation and differentiation of fibroblasts, collagen production, and wound contraction (Khezri et al 2020). On the other hand, oxidative stress increases under wound disorders and increases the production of reactive oxygen species (ROS) in the site of wound (Ustuner et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Accelerative effect of topical Zataria multiflora essential oil against infected wound model by modulating inflammation, angiogenesis, and collagen biosynthesis

Farahpour

Sheikh

Kafshdooz

et al. 2020

Pharmaceutical Biology

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Context: Zataria multiflora Boiss (Lamiaceae) essential oil (ZME) is believed to be a bactericide herbal medicine and might alleviate negative effects of infection. Objective: This study evaluates the effects of an ointment prepared from ZME (ZMEO) on infected wounds. Materials and methods: A full-thickness excisional skin wound was surgically created in each mouse and inoculated with 5 Â 10 7 suspension containing Pseudomonas aeruginosa and Staphylococcus aureus. The BALB/c mice (n ¼ 72) were divided into four groups: (1) negative control that received base ointment (NCG), (2) positive control that daily received Mupirocin V R (MG), (3) therapeutic ointment containing 2% ZMEO and (4) therapeutic ointment containing 4% ZMEO, for 21 days. Wound contraction, total bacterial count, histopathological parameters, antioxidant activity, qRT-PCR analysis for expression of IL-1b, TNF-a, VEGF, IGF-1, TGF-b, IL-10, and FGF-2 mRNA levels were assessed on days 3, 7, and 14 following the wounding. Results: Topical administration of ZMEO significantly decreased the total bacterial count and wound area and also expression of IL-1b and TNF-a compared to the control groups (p < 0.05) in all days. This could also increase significantly the expression of TGF-b, IL-10 IGF-1, FGF-2, and VEGF, and also angiogenesis, fibroblasts, fibrocytes, epithelialization ratio, and collagen deposition and improve antioxidant status compared to the control group (p < 0.05). Discussion and conclusion: ZMEO accelerated the healing process of infected wounds by shortening the inflammatory factors and increasing proliferative phase. Applying ZMEO only and/or in combination with chemical agents for the treatment of wound healing could be suggested.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Accelerative effect of topical Zataria multiflora essential oil against infected wound model by modulating inflammation, angiogenesis, and collagen biosynthesis

Farahpour

Sheikh

Kafshdooz

et al. 2020

Pharmaceutical Biology

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“…In this sense, the encapsulation of natural oils by NLC can be a versatile strategy to preserve its therapeutics properties, prevent degradation, improve the stability, and minimize the toxicity. In fact, there are some works that described the encapsulation of different natural oils as active molecules of NLC with anti-inflammatory ( Carbone et al., 2018 ), antifungal ( Badea et al., 2015a ), UV protection ( Badea et al., 2015b ), antineoplastic ( Venturini et al., 2015 ), and bactericidal properties ( Khezri et al., 2020 ). Although, there is still no report of vegetable oils loaded NLC with anti-CJ activity, until now.…”

Section: Introductionmentioning

confidence: 99%

Nanocarriers From Natural Lipids With In Vitro Activity Against Campylobacter jejuni

Ribeiro

Paula

Rossi

et al. 2021

Front. Cell. Infect. Microbiol.

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Campylobacter jejuni (CJ) is the most prevalent zoonotic pathogen of chicken meat and related products, which may lead to gastroenteritis and autoimmune diseases in humans. Although controlling this bacterium is important, CJ strains resistance against traditional antibiotic therapy has been increased. Vegetable oils and fats are natural biomaterials explored since the Ancient times, due to their therapeutic properties. Nanotechnology has promoted the miniaturization of materials, improving bioavailability and efficacy, while reducing the toxicity of loaded active molecules. In this work, a screening of 28 vegetable oils was firstly performed, in order to select anti-CJ candidates by the disc diffusion test. Thus, the selected liquid lipids were used as active molecules in nanostructured lipid carriers (NLC) formulations. The three resultant systems were characterized in terms of particle size (~200 nm), polydispersity index (~0.15), and zeta potential (~-35mV), and its physicochemical stability was confirmed for a year, at 25°C. The structural properties of NLC were assessed by infrared (FTIR-ATR) and differential scanning calorimetry (DSC) analyses. The spherical nanoparticle morphology and narrow size distribution was observed by transmission electron microscopy (TEM) and field emission scanning electron (FE-SEM) analyses, respectively. Then, the in vitro antimicrobial activity test determined the minimum inhibitory concentration (MIC) of each formulation against CJ strains, in both free (1–3 mg/ml−1) and sessile (0.78 mg/ml−1) forms. Finally, the in vitro biocompatibility of NLC was demonstrated through cell viability using VERO cell line, in which F6 was found twice less cytotoxic than pure olibanum oil. Considering the abovementioned achieved, F6 formulation is able to be evaluated in the in vivo anti-CJ efficacy assays.

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Innovative Encapsulation Strategies for Food, Industrial, and Pharmaceutical Applications

Aanniz,

El Omari,

Elouafy

et al. 2024

Chemistry & Biodiversity

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Encapsulation has emerged as a vitally important tool for protecting the integrity of critical chemicals and improving the delivery mechanisms of natural/synthetic drugs, enabling the controlled release of ingredients and the maintenance of their chemical, physical, and biological properties. It is well known that essential oils (EOs) provide a valuable alternative for food preservation, as they help reducing the deterioration of foodstuffs as well as the proliferation of pathogens. Nevertheless, EOs are highly volatile and lipophilic, rendering them insoluble in aqueous systems. In addition, their secondary metabolites are extremely susceptible to environmental factors such as humidity, temperature, light, and oxygen. Therefore, encapsulation of EOs is an innovative option not only for preserving these substances but also for promoting their stability, controlling their release, and optimizing their efficiency and bioavailability. In this sense, this review aimed to describe current techniques and approaches used to incorporate natural hydrophobic compounds, covering EOs. It also examines whether encapsulation technology can be used efficiently in drug discovery and development. Studies have shown that microencapsulation, the use of nanoparticle, and liposomal are the most effective techniques for encapsulating EOs. Other encapsulation systems included spray drying, coacervation, and emulsification.

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Efficacy of Mentha pulegium essential oil encapsulated into nanostructured lipid carriers as an in vitro antibacterial and infected wound healing agent

Cited by 72 publications

References 34 publications

Accelerative effect of topical Zataria multiflora essential oil against infected wound model by modulating inflammation, angiogenesis, and collagen biosynthesis

Accelerative effect of topical Zataria multiflora essential oil against infected wound model by modulating inflammation, angiogenesis, and collagen biosynthesis

Nanocarriers From Natural Lipids With In Vitro Activity Against Campylobacter jejuni

Innovative Encapsulation Strategies for Food, Industrial, and Pharmaceutical Applications

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