Citation for this version held on GALA:Stevenson, Philip C., D'Cunha, Reju F. and Grzywacz, David (2010) Inactivation of baculovirus by isoflavonoids on chickpea (Cicer arietinum) leaf surfaces reduces the efficacy of nucleopolyhedrovirus against Helicoverpa armigera. London: Greenwich Academic Literature Archive.
ABSTRACT-Biological pesticides based on nucleopolyhedroviruses (NPVs) can provide 14an effective and environmentally benign alternative to synthetic chemicals. On some 15 crops, however, the efficacy and persistence of NPVs is known to be reduced by plant 16 specific factors. The present study investigated the efficacy of Helicoverpa armigera 17 NPV (HearNPV) for control of H. armigera larvae and showed that chickpea reduced the 18 infectivity of virus occlusion bodies (OBs) exposed to the leaf surface of chickpea for at 19 2 least one hr. The degree of inactivation was greater on chickpea than on previously 20 reported on cotton and the mode of action is different to that of cotton. The effect was 21 observed for larvae that consumed OBs on chickpea leaves but also occurred when OBs 22 were removed after exposure to plants and inoculated on to artificial diet, indicating that 23 inhibition was leaf surface related and permanent. Despite their profuse exudation from 24 trichomes on chickpea leaves and low pH, organic acids -primarily oxalic and malic acid 25 -caused no inhibition. When HearNPV was incubated with biochanin A and sissotrin, 26 however, two minor constituents of chickpea leaf extracts, the OB activity was reduced 27 significantly. These two isoflavonoids increased in concentration by up to 3 times within 28 one hr of spraying the virus suspension onto the plants and also when spraying only 29 carrier, indicating induction was in response to spraying and not a specific response to the 30