“…Successive serial dilutions (1:1) of the CHI stock solution were performed in Sabouraud broth (Himedia, India) to obtain solutions containing different final CHI concentrations (8, 4, 2, 1, 0.5, and 0.25 mg/mL). Stock emulsion of CCEO was obtained by dissolving the EO (final concentration 80 µL/mL) in sterile Sabouraud broth containing Tween 80 [1.0%, v/ v (Himedia, India)] as a stabilizing agent (Santos et al, 2012;Sousa et al, 2013). Further, successive dilutions (1:1) of CCEO stock solution in double-concentrated Sabouraud broth were performed to obtain solutions containing different CCEO final concentrations (40, 20, 10, 5, 2.5, 1.2, 0.6, and 0.3 µL/mL).…”