Efficacy of Penicillium Chrysogenum Strain Snef1216 Against Root-Knot Nematodes (Meloidogyne Incognita) in Cucumber (Cucumis Sativus L.) Under Greenhouse Conditions

Abstract

Root-knot nematode (Meloidogyne incognita) has become a serious risk for cucumber globally. Haphazard use of synthetic chemicals poses a serious threat to agricultural crops, eliminating predators and also polluting natural community. Thus these negative effects led towards development of ecofriendly approaches which are safe and effective for M. incognita management. The study has been planned to coat seeds with Snef1216 (Penicillium chrysogenum) and evaluate its ability to control M. incognita in cucumber. It reduced M. incognita invasion rate significantly in different inoculums 500J2 (second stage juveniles) 67.09% followed by 1000J2 and 2000J2 plant -1 60.44% and 36.02%, respectively. It inhibited development of nematodes 60.30%, 50.37% and 38.77% at 500, 1000 and 2000J2 plant -1 inoculation levels respectively compared to control. Snef1216 reduced significant (P<0.05) reproduction rate at 500J2 (69.46%), 1000J2 (62.89%) and 2000J2 (63.62%) of M. incognita. It interfered in galls formation and nematodes g -1 in root mass. Snef1216 enhanced seed germination (22.09%) with germination index (53.77%) and germination rate (64.49%). Additionally, seed dressing with Snef1216 exhibited additional biomass, reduced invasion of second-stage juveniles and also restrained development of nematode. Our results suggest that Snef1216 can be introduced as a biomass enhancer and potential bio-control agent against M. incognita in cucumber.

Mycochemical Screening and Analysis, Antioxidant Activity, and Biochemical Composition of Fermentation Strain Snef1216 (Penicillium chrysogenum)

“…After 3 days, 100 ml of the new nutritious medium was added into each flask and then placed in a shaker for 8 days at 150 rpm and 28°C. Fermentation was filtered and stored at 4°C until use [16][17][18] e subsequent mixture was allowed to settle down for 1 min at room temperature. Addition of 5-6 drops of concentrated ammonia (NH 3 ) into the mixture resulted in pink colour appearance confirmed the presence of glycosides [19].…”

“…Strain Snef1216 (Penicillium chrysogenum) was identified by Jiang et al [16]. Yao et al [17] and Sikandar et al [18] reported its efficiency against Meloidogyne incognita (rootknot nematode) in tomato and cucumber, respectively. However, mycochemical analysis and the presence of biologically active compounds have not been properly studied.…”

Phytochemical screening, GC-MS profiling of invasive cocklebur (Xanthium strumarium)-insect-pathogen interaction and simulated volatile chemical signaling at Northeast China

“…Residuals are the measures that how the values are far away from the regression lines around our data points [121], that measures the best fitness of model validity [122,123]. In this experiment RMSE gave yield performance regarding range of model fitness [115,124].…”

Genistein and Daidzein effects on the physiological indices of Soybean Cyst Nematodes

“…To avoid further penetration of J2s, seedlings were moved to new containers. The number of females of J2s, J3s, and J4s inside the roots at 7 dpi, 14 dpi, 21 dpi, and 28 dpi were observed and counted using a stereomicroscope after the roots were stained with acid fuchsin (Hussey, 1985;Sikandar et al, 2019). The experiment was repeated three times.…”