Efficacy of photodynamic therapy in the management of oral premalignant lesions. A systematic review

Vohra, Fahim; Al-Kheraif, Abdulaziz A.; Qadri, Talat; Hassan, Mohamed Ibrahim Abu; Ahmed, Asma; Warnakulasuriya, Saman; Javed, Fawad

doi:10.1016/j.pdpdt.2014.10.001

Cited by 71 publications

(44 citation statements)

References 32 publications

(235 reference statements)

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“…Studies have reported that aPDT has been effective in the management of oral infections, such as endodontic infections, periimplantitis and periodontitis [23][24][25][26][27][28]. However it is noteworthy that aPDT in these studies [23][24][25][26][27][28] was performed as an adjunctive treatment to conventional mechanical debridement.…”

Section: Discussionmentioning

confidence: 99%

Role of antimicrobial photodynamic therapy in the treatment of aggressive periodontitis: A systematic review

Vohra

Akram

Safii

et al. 2016

Photodiagnosis and Photodynamic Therapy

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Section: Discussionmentioning

confidence: 99%

Role of antimicrobial photodynamic therapy in the treatment of aggressive periodontitis: A systematic review

Vohra

Akram

Safii

et al. 2016

Photodiagnosis and Photodynamic Therapy

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“…Photodynamic therapy (PDT) is a minimally invasive and effective technique that is often used in the treatment of premalignant and malignant lesions in various specialties . PDT consists of three elements: a photosensitizer, oxygen, and light.…”

Section: Introductionmentioning

confidence: 99%

“…Vohra et al reviewed 13 studies using PDT for the treatment of oral premalignant lesions. Eleven of these studies used topical PDT . Tao et al reviewed 14 studies using PDT for the treatment of cervical intraepithelial neoplasia and concluded that the complete response rate was 80.5%, and HPV eradication rate ranged from 53.4% to 80.0%.…”

Section: Introductionmentioning

confidence: 99%

“…However, most studies used systemic administration of photosensitizers to treat laryngeal lesions. The most important reason is that topical application of photosensitizers into the larynx is difficult, and the recommended incubation time of photosensitizers is usually 1 to 5 hours for superficial lesions . Thus far, there are only three studies that attempted topical application of 5‐ALA in the larynx when performing PDT.…”

Section: Introductionmentioning

confidence: 99%

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Topical Application of 5‐Aminolevulinic Acid Is Sufficient for Photodynamic Therapy on Vocal Folds

et al. 2018

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Objectives To evaluate the feasibility of topical photodynamic therapy (PDT) using 5‐aminolevulinic acid (5‐ALA) for vocal fold leukoplakia. Study Design Ex vivo and in vivo. Methods 5‐ALA was applied topically as a 20% solution to ex vivo canine vocal folds. The penetration depth and concentrations of 5‐ALA in tissue were quantified using frozen sectioning and fluorescamine derivatization after 5‐ALA contact incubation or topical spraying. Then, 5‐ALA solution was sprayed on leporine vocal folds once, twice, or given systemically in vivo. Protoporphyrin IX (PPIX) location was visualized using fluorescence microscopy, and PPIX concentrations were measured using a fluorescent quantitative method. Hematoxylin and eosin (H&E) staining was performed to visualize the histological changes of vocal folds after PDT for each group. Results Topical incubation of 15 minutes with 5‐ALA achieved a penetration depth of over 2 mm and similar concentrations within the superficial 500 μm of epithelium, compared with longer incubation times. Topical spraying of 5‐ALA produced sufficient concentrations in vocal folds, but the retention time is short. An in vivo leporine model showed that laryngeal spraying of 20% 5‐ALA induced similar penetration depth and concentrations of PPIX compared to systemic administration of 5‐ALA. Two sprays of 20% 5‐ALA solution with an interval of 30 minutes are needed to produce complete exfoliation of vocal fold epithelium. Conclusion Topical PDT with laryngeal spraying of 20% 5‐ALA solution achieves sufficient therapeutic effects and is potentially applicable for the treatment of vocal fold leukoplakia. Level of Evidence NA Laryngoscope, 129:E80–E86, 2019

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“…Subsequent irradiation with appropriate wavelength light triggers the excitation of the PS and the generation of ROS, mainly singlet oxygen, selectively destroying the target cell through the oxidation of cellular constituents, which damages plasma membranes and subcellular organelles [4,5]. The advantages of PDT over conventional therapies are the relatively high selectivity and low systemic toxicity, and so, PDT is being currently used for the treatment of many cancers such as bladder, brain, lung, gastrointestinal and gynaecological neoplasms, and also in dermatology and non-oncological disorders [6][7][8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Modulation of 5-Aminolevulinic acid mediated photodynamic therapy induced cell death in a human lung adenocarcinoma cell line

Mj¹,

Diez²,

Batlle³

et al. 2016

Integr Cancer Sci Therap

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Photodynamic therapy (PDT) is a cancer treatment involving the administration of a photosensitising drug which selectively accumulates in tumor tissue, followed by irradiation with appropriate wavelength light. It triggers photochemical reactions inducing reactive oxygen species (ROS) production with the consequent cellular damage, which ultimately leads to cell death. Porphyrins are the only photosensitizers (PSs) endogenously synthesized by means of administration of the biological precursor, 5-aminolevulinic acid (ALA). Several antioxidants and ROS scavenger agents: reduced glutathione (GSH), mannitol (Man), l-tryptophan (Trp), ascorbate (Asc) and trolox (Trx), were assayed to determine their ability to modulate ALA-based PDT (ALA-PDT); it was performed on A549 human lung adenocarcinoma cells, by incubating with 1mM ALA for 3 hr and followed by irradiation with or without 1 hr pre-incubation with the modulators. They were previously tested for possible cytotoxicity/ photoactivity in concentrations ranging from 0.01 to 20 mM. The ratio between cell survival after ALA-PDT in the presence and in the absence of the scavenger agent (protection grade: PG) was determined, and the concentration showing no cytotoxicity/ photoactivity and providing the highest PG was used in the subsequent experiments. ALA-PDT alone induced a high percentage of apoptotic cell death (98.4 ± 3.5%) as revealed by acridine orange/ethidium bromide staining and AnnexinV-FITC/propidium iodide labelling. Pre-incubation with the modulators at their highest PG concentration significantly reduced apoptotic cells to 48.3 ± 2.7% (Asc), 58.8 ± 4.2 (Trx), 78.5 ± 3.1% (GSH), 64.3 ± 1.6% (Man), 74.6 ± 2.3% (Trp). ROS involvement in early cell death induction after ALA-PDT was tested by flow cytometry using the fluorescent probes dihydro-dichlorofluorescein diacetate (H2-DCFDA) and methoxyvinylpyrene (MVP) for detection of peroxides and singlet oxygen, respectively. ROS production increased after ALA-PDT (H2-DCFDA positive cells, control: 1.1 ± 0.1 %; 10 min-PDT: 69.3 ± 5.6%; MVP positive cells, control: 0.65 ± 0.35%; 10 min-PDT: 83.5 ± 1.9%). Asc prevented peroxide formation (H2-DCFDA positive cells: 50.7 ± 2.8%) and mostly prevented singlet oxygen increase (MVP positive cells: 25.4 ± 5.2%) whereas Trx limited peroxides formation (H2-DCFDA positive cells: 20.8 ± 0.5%), but did not significantly affected singlet oxygen production (MVP positive cells: 73.6 ± 3.4%). Selective scavenger mediated protection against PDT-induced cell death, and direct detection of specific pro-oxidative agents, entail the strong involvement of ROS in ALA-PDT-mediated tumor eradication, suggesting that undesired photodamage to normal tissue might be attenuated by administration of antioxidant agents.

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Efficacy of photodynamic therapy in the management of oral premalignant lesions. A systematic review

Cited by 71 publications

References 32 publications

Role of antimicrobial photodynamic therapy in the treatment of aggressive periodontitis: A systematic review

Role of antimicrobial photodynamic therapy in the treatment of aggressive periodontitis: A systematic review

Topical Application of 5‐Aminolevulinic Acid Is Sufficient for Photodynamic Therapy on Vocal Folds

Modulation of 5-Aminolevulinic acid mediated photodynamic therapy induced cell death in a human lung adenocarcinoma cell line

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