Efficacy of Prunus Armeniaca on Oral Squamous cell carcinoma Cell Line: An Ex- Vivo study

Abstract

Prunus Armeniaca (Egyptian apricot) is the plant of Rosaceae family which is widely known for its fruit. It has high nutritional value as it contains essential amino acids, proteins, carbohydrates, and lipids. Kernel oil extracted from it was found to have antibacterial, antifungal, antioxidant, and anti-inflammatory effects. Its therapeutic effect is still under research. Apricot extracts were found to inhibit in vitro growth of breast cancer and hepatocellular carcinoma. Objectives: Evaluating the effect of Prunus Armeniaca (PA) extract on Oral Squamous cell carcinoma (OSCC) Methods: Egyptian PA seeds were macerated and added to 90% methanol for 7 day for alcohol extract preparations. OSCC cell line of human tongue were cultured and divided onto 2 groups. Group − 1 was treated with the PA alcohol extract. Group-2 were untreated cell line of the OSCC human tongue as a negative control group. Caspase-3 and − 8 activity assay, Cell viability and cytotoxicity, and Cell cycle analysis were performed. Results: Group-1 showed significant increase in caspase-3 & -8 than group 2. Group-1 showed significant minimal cytotoxicity in comparison to Staurosporine. Cell cycle analysis revealed cell growth arrest in S- phase for group-2 with total apoptosis 7.7 folds than group-1. Conclusion: PA extract yields anti-growth properties on human tongue OSCC cell line.