Efficacy of tacrolimus in inhibiting inflammation caused by carrageenan in a murine model of air pouch

Vigil, Silvana Virgínia Gagliotti; Liz, Rafael de; Medeiros, Yara S.; Fröde, Tânia Sílvia

doi:10.1016/j.trim.2008.01.003

Cited by 40 publications

(33 citation statements)

References 25 publications

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“…The reductions in the expression levels of these genes may explain the effect of tacrolimus on the improvement of steatosis, insulin resistance, and oxidative stress in these HCV models. Although recent investigations have shown that the immunosuppressive drugs tacrolimus and rapamycin inhibit the expression of different inflammatory mediators, 42,43 the anti-inflammatory functions of these drugs are not well established. Our in vitro and in vivo experiments confirmed that tacrolimus inhibited the induction of ROS generation, which is mediated by the core protein.…”

Section: Discussionmentioning

confidence: 99%

Tacrolimus Ameliorates Metabolic Disturbance and Oxidative Stress Caused by Hepatitis C Virus Core Protein

Moriya

Miyoshi

Tsutsumi

et al. 2009

The American Journal of Pathology

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Hepatic steatosis and insulin resistance are factors that aggravate the progression of liver disease caused by hepatitis C virus (HCV) infection. In the pathogenesis of liver disease and metabolic disorders in HCV infection, oxidative stress due to mitochondrial respiratory chain dysfunction plays a pivotal role. Tacrolimus (FK506) is supposed to protect mitochondrial respiratory function. We studied whether tacrolimus affects the development of HCV-associated liver disease using HCV core gene transgenic mice, which develop hepatic steatosis, insulin resistance, and hepatocellular carcinoma. Administration of tacrolimus to HCV core gene transgenic mice three times per week for 3 months led to a significant reduction in the amounts of lipid in the liver as well as in serum insulin. Tacrolimus treatment also ameliorated oxidative stress and DNA damage in the liver of the core gene transgenic mice. Tacrolimus administration reproduced these effects in a dose-dependent manner in HepG2 cells expressing the core protein. The intrahepatic level of tumor necrosis factor-␣, which may be a key molecule for the pathogenesis in HCV infection, was significantly decreased in tacrolimustreated core gene transgenic mice. Tacrolimus

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Section: Discussionmentioning

confidence: 99%

Tacrolimus Ameliorates Metabolic Disturbance and Oxidative Stress Caused by Hepatitis C Virus Core Protein

Moriya

Miyoshi

Tsutsumi

et al. 2009

The American Journal of Pathology

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“…The animals were fixed on a surgical table, and an incision in the dorsal skin was made to perforate the air pouch. The cavity was then washed with 1.0 mL of sterile PBS solution (pH 7.6, containing NaCl (130 mM), Na 2 PO 4 (5 mM) KH 2 PO 4 (1 mM), and heparin (20 IU/mL) in distilled water) (Benincá et al 2007;Vigil et al 2008). The leukocyte count; exudate concentrations; myeloperoxidase and adenosine deaminase activities; NOx, TNF, IFN-γ, IL-2, IL-4, IL-6, IL-10, IL-17, and p-65 phosphorylation levels, and cellular apoptosis were evaluated 24 h after the administration of carrageenan.…”

Section: Air Pouch Modelmentioning

confidence: 99%

“…0.5 h before Cg induction. The inflammation was also analyzed after 24 h. The doses and pretreatment of the reference drugs were chosen based on previous studies in our laboratory and literature (Sedgwick and Lees 1986;Benincá et al 2007;Vigil et al 2008;Dalmarco et al 2009;de Oliveira et al 2014;Dos Reis et al 2014).…”

Section: Air Pouch Modelmentioning

confidence: 99%

“…This analysis was estimated by a colorimetric method using an enzyme immunoassay EIE plate reader (OrganonTeknika, Roseland, NJ, USA) at 600 nm, and interpolation from a standard curve of Evans blue dye ranging from of 0.01 to 50 μg/mL, as described by Benincá et al (2007) and Vigil et al (2008). The results were expressed in microgram per milliliter.…”

Section: Quantification Of Exudation Concentrationsmentioning

confidence: 99%

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Etanercept administration prevents the inflammatory response induced by carrageenan in the murine air pouch model

Mattei

Dalmarco

Fröde

2015

Naunyn-Schmiedeberg's Arch Pharmacol

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Rheumatoid arthritis (RA) is one of several inflammatory and autoimmune diseases that affect approximately 1% of world's population. The development of TNF inhibitors in the last decade represents a great advance in the treatment of mild and severe forms of RA. Etanercept is one of these drugs that is useful for RA treatment, but the mechanism of inhibition of the signaling pathway of inflammation was not completely elucidated. This study was conducted to evaluate the anti-inflammatory effect of etanercept in comparison to reference drugs (dexamethasone and indomethacin). Inflammation was induced by subcutaneal administration of carrageenan in the Swiss albino mice using the murine air pouch model. Exudation; leukocytes; myeloperoxidase (MPO); adenosine deaminase (ADA); nitric oxide metabolites (NOx); tumor necrosis factor (TNF); interferon gamma (IFN-γ); interleukins (IL) IL-6, IL-17, IL-10, IL-4, and IL-2; nuclear transcription factor kappa B (NF-κB) activation and apoptosis were evaluated 24 h after the induction of inflammation. Treatment with etanercept significantly inhibited exudate concentrations; leukocyte count; MPO and ADA activities; NOx, TNF, IFN-γ, and IL-17 levels; and NF-kappa B activation (p < 0.05). Etanercept induced apoptosis, reducing the number of viable neutrophils without increasing necrosis (p < 0.05). Our results suggest that the anti-inflammatory mechanism of action of etanercept may be via decrease of NF-κB activation. This effect promoted the reduction of pro-inflammatory cytokines and NOx and the induction of neutrophil apoptosis. The effect of etanercept upon neutrophils apoptosis may indicate the use of this drug therapy in the early stage of rheumatoid arthritis disease.

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“…Tacrolimus is a macrolide immunosuppressant drug isolated from Streptomyces tsukubaensis and is widely used in organ transplantation (131). Oral tacrolimus for chronic urticaria treatment has been studied in an open-label prospective study (132).…”

Section: Tacrolimusmentioning

confidence: 99%

Treatment of Chronic Urticaria

Tončić

Lipozenčić²,

Marinović³

2009

Urticaria and Angioedema, Second Edition

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Summary Urticaria is a disorder characterized by rapid onset of localized swelling of the skin or mucosa, called wheals or urtica. According to frequency and duration, urticaria can be divided into acute and chronic type. Chronic urticaria is any type of urticaria occurring every day or twice per week, lasting longer than 6 weeks. Chronic urticaria is a common disorder and estimated prevalence is 1% of the population. Also, it is not rare in childhood. The pathogenesis of chronic urticaria has not yet been completely understood. Chronic urticaria is a heterogeneous group of disorders, and according to the etiology and cause, several groups of chronic urticaria are distinguished, i.e. autoimmune, pseudoallergic, infection-related, physical urticaria, vasculitis urticaria and idiopathic urticaria. Treatment and management of chronic urticaria can be non-pharmacological and pharmacological, and sometimes it is not possible to control the disease with antihistamines only, which are considered to be the mainstay of treatment. In severe cases of chronic urticaria, especially if autoimmunity has been proven, several authors describe different modules of immunomodulation: cyclosporine, cyclophosphamide, mycophenolate-mofetil, omalizumab, plasmapheresis, systemic corticosteroids, and immunoglobulin therapy. This article primarily addresses the treatment of chronic idiopathic and autoimmune urticaria.

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Efficacy of tacrolimus in inhibiting inflammation caused by carrageenan in a murine model of air pouch

Cited by 40 publications

References 25 publications

Tacrolimus Ameliorates Metabolic Disturbance and Oxidative Stress Caused by Hepatitis C Virus Core Protein

Tacrolimus Ameliorates Metabolic Disturbance and Oxidative Stress Caused by Hepatitis C Virus Core Protein

Etanercept administration prevents the inflammatory response induced by carrageenan in the murine air pouch model

Treatment of Chronic Urticaria

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