Efficacy of the Aqueous Extracts of Justicia Galeopsis Leaves on the Improvement of Hematological Parameters in Anemic Rats

Abstract

Antianemc potential of aqueous of Justicia galeopsis leaves was studied using Wistar Albino rats after induction of anemia by phenylhydrazine hydrochloride. Forty rats (20 male and 20 female) subdivided into five groups of eight rats were used. There was a group as control (not anemic) and four other anemic groups which had received by gavage respectively 1 ml/kg of distilled water, 1 ml/kg of body weight of Vitafer (reference drug commonly used to treat anemia), 100 mg/kg of body weight of extract of J. galeopsis leaves cooked during 30 min and 30 mg/kg of body weight of extract of J. galeopsis leaves cooked during 45 min. Hematological parameters (red blood cells, hemoglobin and hematocrit) were analyzed as indices of anemia and the weights of specific organs (liver, spleen and kidney) were evaluated. The results of this investigation had showed that aqueous extract of J. galeopsis leaves cooked improved red blood cells, hemoglobin and hematocrit levels. These extracts were not toxic for liver, spleen and kidney. The administration of 100 mg/kg/day of extract of leaves cooked leaves during 30 min promotes a better recovery rate of the number of red blood cells (94.80 %), hemoglobin level (159.53 %) and hematocrit (117.72 %) than Vitafer and the extract of leaves cooked for 45 min. This is suggestive that aqueous extracts of Justicia galeopsis leaves cooked during 30 min may be exploited during 2 weeks in the treatment of anemia.

Biochemical Composition and Anti-anemic Potential of <em>Solanum torvum</em> (Solanaceae) Berries in Albino Wistar Rats

“…However, the aqueous extract of cooked S. torvum berries at the concentration of 6.4 mg/mL resulted in a higher recovery rate on day 15 compared to the reference anaemia drug (95.19% vs 77.12%) (Table 4). Our results are similar to those obtained by Tchogou et al [28] and Loukou et al [18] who observed in their study a higher haemoglobin recovery rate in the batches of rats treated with aqueous extract of Coco nucefera (Arecaceae) and Justicia Galeopsis (Acanthaceae) respectively compared to the control treated with Vitafer. It is known that iron is part of haemoglobin, myoglobin and cytochrome.…”

“…In addition, the aqueous extract of cooked berries resulted in greater erythrocyte recovery on day 15 at concentrations of 3.2 and 6.4 mg/mL compared to Vitafer (+116.43% and +223.64% vs +100%) (Table 3). These results are broadly similar to those obtained by Loukou et al [18] who showed that administration of 1000 mg/kg/d of Justicia Galeopsis leaf extract cooked for 30 min increased erythrocyte with a recovery of +98.11%. The extract of leaves cooked for 30 min allows a better stimulation of red blood cell synthesis.…”