Efficacy of the essential oil of Origanum glandulosum Desf against skin infections With PVL-producing Staphylococcus aureus strains in mice

Abstract

Skin and soft tissue infections (SSTI) caused by staphylococcal toxins including Panton-Valentine leukocidin (PVL) have increased these last years. Treatment of these complicated infections is often difficult. The search for new methods and new antimicrobial substances is necessary. Natural products such as essential oils appear among the possible solutions. The objective of our work was to prove the effectiveness of treatment of these infections with essential oils in vivo on Wistar rats; we have chosen the oil of Origanum glandulosum from Algeria to perform this treatment because of its antiseptic properties. The treatment was done on infections induced in Wistar rats using PVL producing Staphylococcus aureus strain isolated from Tlemcen University Hospital. The results of our experiment have shown a significant decrease in the number of colonies collected from rats treated by the concentrations of 1 and 5% of O. glandulosum essential oil. The treatment of skin infections especially wounds caused by PVL positive S. aureus is possible with the essential oil of O. glandulosum which seems to be effective in eradicating the germ from infected wounds.

Essential Oil of Thymus munbyanus subsp. coloratus from Algeria: Chemotypification and in vitro Biological Activities

“…This chemotype has never been reported for T. munbyanus before. In fact, previous studies conducted on plant samples collected in different areas of Algeria reported carvacrol (71.4 – 80.3%), carvacrol (35.2%) and thymol (18.5%), thymol (44.2%), thymol (37.7%) and p ‐cymene (14.7%), thymol (52.0 – 60.5%) and γ ‐terpinene (9.5 – 11.3%), thymol (44.2%), ( E )‐ β ‐ocimene (25.8%) and α ‐terpinene (12.3%), camphor (21.8 – 24.9%), camphene (13.5 – 17.3%) and 1,8‐cineole (11.9 – 15.7%), as the major essential oil constituents. Only in a few studies, the subspecies investigated (subsp.…”

“…coloratus gave yield values of 0.2% and 0.1%, respectively, that are lower than those previously reported for the species, e.g . 6.0%, 5.1%, 3.0%, 2.8%, 2.3%, 2%, 1.8% . We assume that these differences may partially depend on a different glandular indumentum of the subsp.…”

“…Significant quali‐ and quantitative differences were detected. First of all, we identified a total of 109 compounds against 52, 44, 33, 30, 28, 24, 11 and 5 components found in these studies. We assume that these differences may depend on genetic ( e.g .…”

“…However, in most cases only the subsp. ciliatus was analysed, while for the rest of the studies the subspecific level was not determined . In the above studies the most abundant volatile components reported were the phenols thymol and carvacrol, and their precursors p ‐cymene and γ ‐terpinene.…”

“…In the above studies the most abundant volatile components reported were the phenols thymol and carvacrol, and their precursors p ‐cymene and γ ‐terpinene. In some of the above studies the antioxidant and antimicrobial activities of the essential oil have been investigated as well …”