Efficacy of the porcine species in biomedical research

Gutierrez, Karina; Dicks, Naomi; Glanzner, Werner Giehl; Agellon, Luis B.; Bordignon, Vilceu

doi:10.3389/fgene.2015.00293

Cited by 163 publications

(134 citation statements)

References 113 publications

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“…Several inbred lines were selected as homozygous for SLA in NIH miniature pigs for biomedical studies 38 . Previous and current studies showed the establishment of homozygosity in class II (observed heterozygosity, DRB1-0%; DQA1-0%; DQB1-34.72%) but not in class I genes (observed heterozygosity, SLA-1, 83% and SLA-2, 46.6%) 18,[20][21][22] for the breed.…”

Section: Discussionmentioning

confidence: 99%

SLA-1 Genetic Diversity in Pigs: Extensive Analysis of Copy Number Variation, Heterozygosity, Expression, and Breed Specificity

Choi

Lee

et al. 2020

Sci Rep

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Swine leukocyte antigens play indispensable roles in immune responses by recognizing a large numberof foreign antigens and thus, their genetic diversity plays a critical role in their functions. in this study, we developed a new high-resolution typing method for pig SLA-1 and successfully typed 307 individuals from diverse genetic backgrounds including 11 pure breeds, 1 cross bred, and 12 cell lines. We identified a total of 52 alleles including 18 novel alleles and 9 SLA-1 duplication haplotypes, including 4 new haplotypes. We observed significant differences in the distribution of SLA-1 alleles among the different pig breeds, including the breed specific alleles. SLA-1 duplication was observed in 33% of the chromosomes and was especially high in the biomedical model breeds such as SNU (100%) and NIH (76%) miniature pigs. Our analysis showed that SLA-1 duplication is associated with the increased level of SLA-1 mRnA expression in porcine cells compared to that of the single copy haplotype. therefore, we provide here the results of the most extensive genetic analysis on pig SLA-1. open Scientific RepoRtS | (2020) 10:743 | https://doi.org/10.1038/s41598-020-57712-5www.nature.com/scientificreports www.nature.com/scientificreports/ SLA-1, SLA-2, and SLA-3 are constitutively expressed classical MHC class I genes, but their expression may vary depending on the genetic differences. For example, SLA-1 is duplicated in the haplotypes Hp-2.0, Hp-8.0, Hp-11.0, Hp-12.0, Hp-19.0, Hp-20.0, and Hp-27.0 14,24-26 . In addition, SLA-1, 3, and 6 were not expressed in the haplotypes Hp-3.0, Hp-2.0, and Hp-5.0, respectively 24 . Recently, a method was reported to estimate the copy number of SLA-1 and to facilitate our understanding on the functional aspect of SLA-1 duplication 27 . The frequency of SLA-1 duplication could be abundant, but the detailed functional analysis is not been available.Therefore, we developed a genomic DNA based high resolution SLA-1 typing method with high accuracy regardless of CNVs and present the extensive analysis results of SLA-1 diversity including new alleles and haplotypes, and allelic distribution among different breeds. We also analyzed the level of SLA-1 expression in pig cells according to their copy numbers which could affect MHC class I-specific immune responses. The information presented in this study should contribute to improving our understanding on the genetic polymorphisms of SLA-1 in diverse pig breeds. ResultsDetermination of the SLA-1 specific region. To develop a genomic DNA-based typing method of SLA-1, the determination of conserved locus specific region to design SLA-1 specific primers is required. We previously reported the locus specific nucleotide sequence variations at the downstream promoter region from six classical SLA class I-related genes including . Here, we extended the results by incorporating genomic sequences from additional cloning and sequence analysis. As a result, we identified a SLA-1 specific motif between the TATA box and the CAP site in the 5ʹ UTR and designe...

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Section: Discussionmentioning

confidence: 99%

SLA-1 Genetic Diversity in Pigs: Extensive Analysis of Copy Number Variation, Heterozygosity, Expression, and Breed Specificity

Choi

Lee

et al. 2020

Sci Rep

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“…Porcine strains currently available for research include Yorkshire and Landrace farm pigs, and several minipig breeds such as Yucatan, Hanford, Sinclair, and Göettigen minipigs. 53 Young adult Yucatan minipigs weighing approximately 20-30 kg can grow to 50 kg at 9 months of age and will for Göettigen minipigs is roughly 40-50 kg. 53 Our lab has used domestic farm pigs (Yorkshire strain) for short-term studies, e.g.…”

Section: Discussionmentioning

confidence: 99%

“…53 Young adult Yucatan minipigs weighing approximately 20-30 kg can grow to 50 kg at 9 months of age and will for Göettigen minipigs is roughly 40-50 kg. 53 Our lab has used domestic farm pigs (Yorkshire strain) for short-term studies, e.g. investigating the translatability of various tissue engineered nerve grafts from the rodent to porcine model and the mechanism of action of nerve regeneration following short-gap repair at acute time points.…”

Section: Discussionmentioning

confidence: 99%

A Porcine Model of Peripheral Nerve Injury Enabling Ultra-Long Regenerative Distances: Surgical Approach, Recovery Kinetics, and Clinical Relevance

Burrell

Browne

Dutton

et al. 2019

Preprint

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AbstractApproximately 20 million Americans currently experience residual deficits from traumatic peripheral nerve injury. Despite recent advancements in surgical technique, peripheral nerve repair typically results in poor functional outcomes due to prolonged periods of denervation resulting from long regenerative distances coupled with relatively slow rates of axonal regeneration. Development of novel surgical solutions requires valid preclinical models that adequately replicate the key challenges of clinical peripheral nerve injury. Our team has developed a porcine model using Yucatan minipigs that provides an opportunity to investigate peripheral nerve regeneration using different nerves tailored for a specific mechanism of interest, such as (1) nerve modality: motor, sensory, and mixed-modality; (2) injury length: short versus long gap; and (3) total regenerative distance: proximal versus distal injury. Here, we describe a comprehensive porcine model of two challenging clinically relevant procedures for repair of long segmental lesions (≥ 5 cm) – the deep peroneal nerve repaired using a sural nerve autograft and the common peroneal nerve repaired using a saphenous nerve autograft – each featuring ultra-long total regenerative distances (up to 20 cm and 27 cm, respectively) to reach distal targets. This paper includes a detailed characterization of the relevant anatomy, surgical approach/technique, functional/electrophysiological outcomes, and nerve morphometry for baseline and autograft repaired nerves. These porcine models of major peripheral nerve injury are suitable as preclinical, translatable models for evaluating the efficacy, safety, and tolerability of next-generation artificial nerve grafts prior to clinical deployment.

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“…Researchers have used multicore and graded index multimode fibres for the delivery of ultrashort pulse (USP) to demonstrate the laser ablation. Prior work has established pig tissue as an efficient medical model for several studies related to human diseases and is consequently applied in porcine vocal fold tissue resection using 1.5 ps laser pulses delivered through kagome lattice hollow core photonic crystal fibre for microsurgery . The microsurgery laser ablation on the vocal fold tissue has a depth and width of ablation of around 43 and 40 μm, respectively, at a pulse energy of 1.2 μJ.…”

Section: Introductionmentioning

confidence: 99%

Preclinical evaluation of porcine colon resection using hollow core negative curvature fibre delivered ultrafast laser pulses

Mohanan

Beck

West

et al. 2019

Journal of Biophotonics

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Ultrashort pulse lasers offer great promise for tissue resection with exceptional precision and minimal thermal damage. Surgery in the bowel requires high precision and minimal necrotic tissue to avoid severe complications such as perforation. The deployment of ultrashort lasers in minimally invasive or endoscopic procedures has been hindered by the lack of suitable optical fibres for high peak powers. However, recent developments of hollow core microstructured fibres provide potential for delivery of such pulses throughout the body. In this study, analysis of laser ablation via a scanning galvanometer on a porcine colon tissue model is presented. A thermally damaged region (<85 μm) and fine depth control of ablation using the pulse energies 46 and 33 μJ are demonstrated. It is further demonstrated that such pulses suitable for precision porcine colon resection can be flexibly delivered via a hollow core negative curvature fibre (HC-NCF) and again ablation depth can be controlled with a thermally damaged region <85 μm. Ablation volumes are comparable to that of early stage lesions in the inner lining of the colon. This study concludes that the combination of ultrashort pulses and flexible fibre delivery via HC-NCF present a viable route to new minimally invasive surgical procedures. K E Y W O R D S negative curvature fibre, plasma-mediated ablation, porcine colon, thermal necrosis, ultrafast laser surgery

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Efficacy of the porcine species in biomedical research

Cited by 163 publications

References 113 publications

SLA-1 Genetic Diversity in Pigs: Extensive Analysis of Copy Number Variation, Heterozygosity, Expression, and Breed Specificity

SLA-1 Genetic Diversity in Pigs: Extensive Analysis of Copy Number Variation, Heterozygosity, Expression, and Breed Specificity

A Porcine Model of Peripheral Nerve Injury Enabling Ultra-Long Regenerative Distances: Surgical Approach, Recovery Kinetics, and Clinical Relevance

Preclinical evaluation of porcine colon resection using hollow core negative curvature fibre delivered ultrafast laser pulses

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