Efficacy of Three Different Lasers on Eradication of<i>Enterococcus faecalis</i>and<i>Candida albicans</i>Biofilms in Root Canal System

Kasić, Silvija; Knezović, Marita; Beader, Nataša; Gabrić, Dragana; Malčić, Ana Ivanišević; Baraba, Anja

doi:10.1089/pho.2016.4258

Cited by 31 publications

(26 citation statements)

References 29 publications

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“…Our findings fit with the observation of Kasic et al 27 who reported a 10,000-fold reduction of C. albicans in the presence of E. faecalis using Er:YAG laser irradiation of NaCl inside root canals. The authors did not explain their findings, but it can be speculated that mixing these two strains might lead to different growth and dynamics of the biofilm or to different susceptibility to fluid dynamics.…”

Section: Endodontic Disinfection With Er:yag Laser Discussionsupporting

confidence: 93%

“…This is in line with Sahar-Helft et al 25 who reported that Er:YAG LAI did not improve CFUs of E. faecalis biofilm compared with manual dynamic irrigation, and also Ordinola-Zapata et al 26 reported better antibacterial effects on E. faecalis with the use of needle irrigation of NaOCl than the use of LAI. The same was reported by Kasic et al, 27 who found a significant but clinically irrelevant 10% reduction of E. faecalis counts in artificially infected root canals. However, it was reported that, using SEM analysis as validation method, Er:YAG irradiation of NaCl solution inside the root canal was able to partially remove the E. faecalis biofilm from root canal walls and to expose dentinal tubules.…”

Section: Endodontic Disinfection With Er:yag Laser Discussionsupporting

confidence: 85%

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In VitroEffect of Er:YAG Laser on Different Single and Mixed Microorganisms Being Associated with Endodontic Infections

Henninger

Berto

Eick

et al. 2019

Photobiomodulation, Photomedicine, and Laser Surgery

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Objective: The purpose of this in vitro study was to evaluate the antimicrobial effect of activated irrigation with different modes of erbium-doped yttrium aluminum garnet (Er:YAG) laser application on microorganisms related to secondary endodontic infection. Background: Er:YAG laser has been recommended as an adjuvant tool for root canal disinfection during endodontic treatment. Materials and methods: Laser-activated irrigation (LAI) with 300 or 600 lm tips were tested with or without intermittent irrigation with 0.9% sodium chloride (NaCl) solution against different microorganisms (five single strains and dual species (Streptococcus gordonii combined with Actinomyces oris or Fusobacterium nucleatum) in root canals after 3 days of incubation. In a 21-day infection model, LAI was used together with intermittent rinsing with sodium hypochlorite (NaOCl) against the dual-species mixtures; here the incidence of microbial regrowth after up to 7 days was monitored. Results: In the 3-day root infection model, LAI protocols did not show any significant reduction of the microbial load when compared with manual irrigation with saline solution. In the 21-day infection, S. gordonii combined with A. oris were not detectable anymore after applying the LAI protocol with a 600 lm tip (30 mJ/ 10 pps) up to 7 days after treatment. Conclusions: Application of LAI with a 600 lm tip by using an Er:YAG laser might be advantageous in treatment of endodontic infections.

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Section: Endodontic Disinfection With Er:yag Laser Discussionsupporting

confidence: 93%

Section: Endodontic Disinfection With Er:yag Laser Discussionsupporting

confidence: 85%

In VitroEffect of Er:YAG Laser on Different Single and Mixed Microorganisms Being Associated with Endodontic Infections

Henninger

Berto

Eick

et al. 2019

Photobiomodulation, Photomedicine, and Laser Surgery

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“…The results were tested and compared to CSI, [42][43][44] diode laser irradiation, 45 PUI, 44 UAI, 46 and Er,Cr:YSGG LAI. 47 One study varied the laser settings and NaOCl concentration and concluded that the decontamination was only efficient with 5% NaOCl and no irrigation protocol other than PIPS (Table 2). 48 Smear Layer Removal Seventeen studies were included in the analysis of smear layer removal.…”

Section: Debris Removalmentioning

confidence: 99%

The Efficiency of the Er: YAG Laser and PhotonInduced Photoacoustic Streaming (PIPS) as an Activation Method in Endodontic Irrigation: A Literature Review

Linh

Gaudin

2020

J Lasers Med Sci

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In the field of endodontics, lasers have been used for a long time for the optimization of Endodontic irrigation in particular. The laser-activated irrigation (LAI) technique is based on the photomechanical effects of the lasers at low settings. They create specific cavitation phenomena and acoustic streaming in intracanal fluids. More recently, a new technique with a Er:YAG laser has been used with sub-ablative energy (20 mJ, 15 Hz) and ultra-short pulses (50 µs). This leads to intracanal cavitation and shockwaves as a result of photoacoustic and photomechanical effects. This phenomenon is called photon-induced photoacoustic streaming (PIPS). PIPS and Laser activated irrigation are described in the literature as a revolutionary and powerful method to activate the irrigant; however, systematic literature on this topic is missing. This review compares the literature on Er:YAG LAI and PIPS on endodontic irrigation with other irrigation methods. An article search was performed on the PubMed database using a series of keywords related to endodontic irrigation, including Er:YAG LAI and PIPS; 59 articles were selected for the review according to the inclusion and exclusion criteria. No in vivo study was found. The Er:YAG LAI and PIPS outperformed other methods in 33 of the 59 articles. There was a great variety in the study designs including bacterial incubation time, laser parameters, irrigation protocols, and irrigating solution used. The evidence suggests that the Er:YAG LAI and PIPS are promising in canal disinfection as well as debris and smear layer removal. However, the large variety in the study methods makes the results less significant. Further studies are needed to better evaluate the efficiency of these techniques, especially in vivo studies.

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“…aPDT is two stage process, this involves delivery of photosensitizer dye at first stage and then application of laser light, this produces a toxic photochemistry, leading to microbial destruction [45,46,47]. Successful eradication of various clinically relevant microbes notably S. mutans and E. faecalis have been reported using aPDT approach, with effective destruction of 97-99.9% of planktonic bacterial loads [48,49].…”

Section: Apdt In Endodonticsmentioning

confidence: 99%

Anti-microbial Photo Dynamic Therapy (aPDT) in Endodontics: A Literature Review

Ahmad¹

2018

IJDSR

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Elimination of root canal pathogens is one of the main objectives for successful outcome of root canal treatment. Conventional chemo-mechanical debridement is not always successful in removing all bacteria. Adjunctive use of antimicrobial photodynamic therapy (aPDT) can be a used as a practical approach for successful elimination of all these bacteria. The aim of this review was to assess scientific literature that used various photosensitizers for bacterial reduction using aPDT methods. A search of the PubMed and Cochrane data bases using appropriate terms and keywords related to antimicrobial photodynamic therapy in endodontic disinfection was conducted. A hand search also was conducted in the preceding 5 years of several journals. Full texts of the selected articles were obtained and analyzed. A total of 177 articles published were identified at first stage of data extraction. After final screening, 41 articles were included in this review. This review summarizes the usage principles of aPDT and outlines the applications of aPDT in root canal disinfection procedures. It was concluded that aPDT should be used in conjunction with the conventional chemo mechanical debridement and disinfection of root canal system. It is critical to note that selection of photosensitizer dye, its correct delivery within the prepared root canal system, laser application with correct type of tips are predictors for successful outcome of this protocol of root canal disinfection.

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Efficacy of Three Different Lasers on Eradication ofEnterococcus faecalisandCandida albicansBiofilms in Root Canal System

Abstract: Er,Cr:YSGG laser was the most efficient tool in eradication of E. faecalis and C. albicans biofilms.

Cited by 31 publications

References 29 publications

In VitroEffect of Er:YAG Laser on Different Single and Mixed Microorganisms Being Associated with Endodontic Infections

In VitroEffect of Er:YAG Laser on Different Single and Mixed Microorganisms Being Associated with Endodontic Infections

The Efficiency of the Er: YAG Laser and PhotonInduced Photoacoustic Streaming (PIPS) as an Activation Method in Endodontic Irrigation: A Literature Review

Anti-microbial Photo Dynamic Therapy (aPDT) in Endodontics: A Literature Review

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