Efficacy of Trichoderma against Sclerotium rolfsii causing collar rot disease of lentil under in vitro conditions

Abstract

Three biocontrol agents viz., Trichoderma viride, T. virens and T. harzianum were evaluated to test the antagonism against Sclerotium rolfsii under in vitro conditions. All the three antagonists' viz., T. viride, T. virens and T. harzianum have shown the potential of parasitizing the growth of Sclerotium rolfsii in vitro. The rate of inhibition was fastest in T. harzianum (63.60%) followed by T virens (51.5 %). Least inhibition was recorded in T. viride (50.85% ) after 72 hours of incubation. However, T. viride showed the highest (91.31%) reduction in sclerotia formation followed by T. harzianum (84.92%) and T. virens (84.29%) after 15 days of incubation. The volatile compounds from Trichoderma viride were found most effective in suppressing the mycelial growth (51.11%) and sclerotia production (95.90%) of the target pathogen. The culture filtrate from both T. harzianum and T. viride (15% concentration) was found very effective in inhibiting the radial growth (57.46 and 49.62%) and sclerotia formation (98.20 and 99.83%) of Sclerotium rolfsii. The antagonists such as T. harzianum and T. viride can be used as a bio-control agent against S. rolfsii under field condition.

Effect of Metarhizium anisopliae (MetA1) on growth enhancement and antioxidative defense mechanism against Rhizoctonia root rot in okra

“…2 ), this study also confirmed that MetA1 produces antifungal metabolites. This was in line with previous reports, which found that CFCs produced by hypocrealean fungi were effective at inhibiting the growth of fungal pathogens [ 21 , 23 , 49 , 50 ]. A recent study found that CFCs of M. anisopliae containing active toxic compounds and secondary metabolites damaged the mycelium and conidial plasma membrane integrity of the mold-causing fungus Botrytis cinerea, respectively [ 21 ].…”

Morphological and Cultural Characterization of Sclerotium rolfsii Sacc on Chickpea and Its Management Using Combined Fungicide Molecules

“…Similar results were also observed in studies by [21] where they evaluated "effects of different culture media on S. rolfsii mycelial growth, sclerotial production". [22] examined "total of seven media were used for studying the growth of S. rolfsii in Lentil" and [23,24] also proved "the efficiency of PDA in enhancing mycelial growth of fungus out of nine different cultural media. Their results reported that potato-dextrose medium was most suitable for mycelial growth and sclerotia production of S. rolfsii.…”

The Evaluation of Trichoderma consortia against Fusarium udum causing wilt of Pigeonpea

“…The principal biocontrol mechanisms involved include mycoparasitism, antibiosis, competition, and induced resistance ( Kumar et al 2009, Kumar. 2013, Chaudhary et al, 2017, Kushwaha et al, 2018. Application of bioagents in a consortium may improve the efficacy, reliability and consistency of the bioagents even under diverse soil and environmental conditions (Sharma et al 2012…”