Abstract:An experimental challenge trial was performed against Staphylococcus aureus and Streptococcus agalactiae following the procedures recommended by the National Mastitis Council. The efficacy of two teat dips, product 1 (Bovadine with I-Tech II) and product 2 (Bovadine with I-Tech, used as a positive control), was determined. Both teat dips contain 1% iodine and 10% glycerin. Product 1 established an 89.7% reduction in infections against Staph. aureus and 73.1% reduction in infections against Strep. agalactiae. P… Show more
“…Foret et al . () and Boddie et al . () additionally used a cotton pledget moistened with 70% w/v alcohol to wipe each teat, which is somewhat similar to the Animal Health Ireland (AHI) CellCheck recommendations (CellCheck, ).…”
Section: In Vivo Testingmentioning
confidence: 85%
“…The criteria described by Foret et al . () and Boddie et al . () would be preferred by the authors as the most suitable criteria for defining new IMIs.…”
Section: In Vivo Testingmentioning
confidence: 85%
“…(), Foret et al . () and Boddie et al . () used negative controls by leaving the other half of the udder as undisinfected controls.…”
Section: In Vivo Testingmentioning
confidence: 85%
“…All reviewed studies are in agreement with using the split udder design when applying the teat disinfectant, with Foret et al . () and Leslie et al . () also implementing a split herd design.…”
The prevention of mastitis is the most important part of a mastitis control programme. Postmilking disinfection is considered the most effective procedure for preventing new intramammary infections in dairy herds. This article reviews the different protocols used to evaluate the efficacy of teat disinfectant products. These protocols include experimental challenge, natural exposure and excised teat, along with agar diffusion assays and teat swabbing. The experimental designs, limitations, positive and negative aspects of each protocol were compared and discussed throughout this article.
“…Foret et al . () and Boddie et al . () additionally used a cotton pledget moistened with 70% w/v alcohol to wipe each teat, which is somewhat similar to the Animal Health Ireland (AHI) CellCheck recommendations (CellCheck, ).…”
Section: In Vivo Testingmentioning
confidence: 85%
“…The criteria described by Foret et al . () and Boddie et al . () would be preferred by the authors as the most suitable criteria for defining new IMIs.…”
Section: In Vivo Testingmentioning
confidence: 85%
“…(), Foret et al . () and Boddie et al . () used negative controls by leaving the other half of the udder as undisinfected controls.…”
Section: In Vivo Testingmentioning
confidence: 85%
“…All reviewed studies are in agreement with using the split udder design when applying the teat disinfectant, with Foret et al . () and Leslie et al . () also implementing a split herd design.…”
The prevention of mastitis is the most important part of a mastitis control programme. Postmilking disinfection is considered the most effective procedure for preventing new intramammary infections in dairy herds. This article reviews the different protocols used to evaluate the efficacy of teat disinfectant products. These protocols include experimental challenge, natural exposure and excised teat, along with agar diffusion assays and teat swabbing. The experimental designs, limitations, positive and negative aspects of each protocol were compared and discussed throughout this article.
“…Therefore, the many therapeutic methods have been reported, such as antibiotics [8,28,29], cytokines [30,35], immunoglobulin [1], vaccines [27,34], disinfectants [10,11,38] and biologically active substrates [16,19].…”
ABSTRACT. We examined combination therapy with both lactoferrin (Lf) and antibiotics on clinical mastitis due to Staphylococcus aureus (S.aureus) on drying cows. The clinical symptoms of mastitic quarters were cured 81% of combination therapeutic quarters at 7 days post injection (dpi). Moreover, most of mammary gland secretions (MGSs) in combination therapeutic quarters were normal at 7 days after parturition. In the quarters with combination therapy, S.aureus counts, Lf concentrations and content rate of concanavalin A (Con A) low-affinity Lf decreased and were lower than in the quarters treated with Lf or antibiotics alone. The mRNA expression of tumor necrosis factor α (TNFα) of the quarters with combination therapy also decreased and was lower than that of the Lf or antibiotics treated. The mRNA expression of pro-inflammatory cytokines and chemokines in bovine mammary gland epithelial lined cells (BMEC) stimulated with Lf were lower than those of Con A low-affinity Lf stimulated BMEC. Moreover, Lf showed an inhibitory effect to the induction of pro-inflammatory cytokine mRNA expression when co-stimulated with Lf and Con A low-affinity Lf. Nuclear factor kappa B (NFκB) activation was also induced with Con A low-affinity Lf, and the inhibitory effects of Lf were also confirmed on BMEC co-stimulated with Lf and Con A low-affinity Lf. These results indicated that the efficacy of combination therapy with antibiotics and Lf caused antibacterial effect of antibiotics and inhibition of pro-inflammatory cytokine and chemokine production with Lf via the inhibition of NFκB activation.
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