Efficacy of virus elimination from in vitro-cultured sand pear (Pyrus pyrifolia) by chemotherapy combined with thermotherapy

Hu, Guang‐Wan; Hóng, Ní; Wang, L.P.; Hu, Hongju; Wang, G.P.

doi:10.1016/j.cropro.2012.02.017

Cited by 43 publications

(29 citation statements)

References 41 publications

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“…Szittya et al (2003) and Qu et al (2005) reported that plant-virus interactions are strongly affected by temperature, and high temperatures are frequently associated with low virus content in infected plants. In the present study, the survival rate of treated (Paprstein et al 2008;Tan et al 2010;Hu et al 2012). The temperature range is another important factor affecting shoot survival.…”

Section: Discussionmentioning

confidence: 98%

“…When choosing a temperature, there must be a balance between relatively low shoot mortality at higher temperatures and the high percentage of virus-free shoots (Dziedzic 2008). The duration of thermotherapy treatments is another factor in the virus elimination efficiency (Tan et al 2010;Hu et al 2012). Successful in vitro heat treatments used to eliminate ACLSV and ASGV from apple were more than 1 month long (Knapp et al 1995;da Camara Machado et al 1998).…”

Section: Discussionmentioning

confidence: 99%

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Virus elimination from in vitro apple by thermotherapy combined with chemotherapy

Dong

Zhang

et al. 2015

Plant Cell Tiss Organ Cult

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We evaluated the effectiveness of thermotherapy at different temperatures, chemotherapy with different concentrations of ribavirin, and combinations of these two methods on virus elimination from apple. We used Malus cv. 'Xinhongjiangjun', an apple cultivar widely grown in China, infected with Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV), and Apple stem pitting virus (ASPV). The survival and regeneration of plants were evaluated, and the efficiency of virus eradication was determined by reverse-transcription polymerase chain reaction with two primer pairs for each virus. All of the plants treated with 15 and 25 lg/ml ribavirin survived, although their proliferation was slightly inhibited. Ribavirin treatments at 15 and 25 lg/ml resulted in virus elimination rates of 74.4 and 75.0 %, respectively. Higher temperatures significantly affected the growth and proliferation of plants, and almost no axillary shoots regenerated under the highest temperature (38 ± 0.5°C). The average virus elimination rate after 34-36°C treatments for 20 days was no more than 45 %. The virus elimination efficiency was enhanced by combining thermotherapy and chemotherapy treatments. A treatment combining ribavirin (25 lg/ml) and thermotherapy at 36°C (R25 ? T36) resulted in high virus eradication efficiency (95.0 %). Across all of the treatments, the average survival rate of apical shoots (176/273) was 12 % lower than that of axillary shoots (266/349); the average virus elimination efficiency was 65.3 % for apical shoots and 72.7 % for axillary shoots. The results also demonstrated that it was easier to eliminate ASPV than to eliminate ACLSV and ASGV.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Virus elimination from in vitro apple by thermotherapy combined with chemotherapy

Dong

Zhang

et al. 2015

Plant Cell Tiss Organ Cult

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“…Penggunaan bibit bebas virus menjadi salah satu cara pengendalian patogen virus yang efektif (Hu et al, 2012), terutama ketika infeksi virus tidak terjadi secara tunggal melainkan secara bersama-sama oleh beberapa virus. Teknik kultur meristem merupakan cara yang paling umum diaplikasikan untuk mengeliminasi virus (Retheesh dan Bhat, 2010).…”

Section: Pendahuluanunclassified

Aplikasi Teknik Embriogenesis Somatik dan Krioterapi untuk Eliminasi Virus SCSMV pada Tanaman Tebu

Roostika¹,

Hartono²,

Sukmadjaja³

2018

J. AgroBiogen

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The use of virus-free seedlings is an effective option to control viral disease. It was reported that several tissue culture methods could eliminate virus infection from plant tissues. The objective of the study was to know the in vitro responses of three sugarcane varieties to the organogenesis and cryotherapy techniques and to know the potential rate of both techniques in eliminating Sugarcane streak mosaic virus (SCSMV). The plant materials were virus-infected sugarcane of three varieties of sugarcane (PS862 from Cirebon, PS881 from Jember, and PSJK922 from Malang). Callus induction was conducted on MS medium with addition of 3 mg/l 2,4-D and 3 g/l casein hydrolysate. There were three treatments of subculture (SK0, SK1, and SK2). Regeneration of shoots was conducted on MS medium with addition of 0.3 mg/l BA, 0.5 mg/l IBA, and 100 mg/l PVP. Cryotheraphy was done by using vitrification technique, starting with preculture on 0.3 M sucrose-containing medium for 3 days, loading with LS solution for 10 minutes, dehydration with PVS2 solution for 40 minutes, plunging in liquid nitrogen for 1 hour, deloading with RS solution for 30 minutes, recovery, and regeneration. Virus indexing was conducted by RT-PCR assay using specific primer of SCSMV. The result showed that all varieties were able to form for calli and shoots, both with and without subculture. Within the three varieties, only PS862 from Cirebon could survive post cryotherapy treatment. Organogenesis regeneration up to twice subculture was not able to eliminate SCSMV. Cryotherapy could eliminate SCSMV virus with the proportion of one third (33.3%).Keywords: Saccharum officinarum L., embryogenic callus, vitrification, liquid nitrogen, virus elimination. ABSTRAKPenggunaan bibit bebas virus menjadi salah satu cara pengendalian penyakit virus yang efektif. Beberapa macam teknik kultur jaringan dilaporkan mampu mengeliminasi virus di dalam jaringan tanaman. Tujuan penelitian adalah mengetahui respons tiga varietas tebu terhadap teknik organogenesis dan krioterapi serta untuk mengetahui kemampuan kedua teknik tersebut dalam mengeliminasi Sugarcane streak mosaic virus (SCSMV). Bahan tanaman yang digunakan adalah varietas tebu PS862 asal Cirebon, PS881 asal Jember, dan PSJK922 asal Malang yang terinfeksi SCSMV. Induksi kalus dilakukan dengan menggunakan media MS yang ditambah dengan 2,4-D 3 mg/l dan kasein hidrolisat 3 g/l. Terdapat tiga macam perlakuan subkultur (SK0, SK1, dan SK2). Regenerasi tunas dilakukan pada media MS dengan penambahan BA 0,3 mg/l, IBA 0,5 mg/l, dan PVP 100 mg/l. Teknik krioterapi yang diterapkan adalah vitrifikasi dengan tahapan prakultur menggunakan sukrosa 0,3 M selama 3 hari, loading dengan larutan LS selama 10 menit, dehidrasi dengan larutan PVS2 selama 40 menit, krioterapi dengan nitrogen cair selama 1 jam, deloading dengan larutan RS selama 30 menit, pemulihan, dan regenerasi. Indeksing virus dilakukan secara RT-PCR menggunakan primer spesifik SCSMV. Hasil penelitian menunjukkan bahwa ketiga varietas mampu membentuk kalus dan t...

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“…Keberhasilan eliminasi virus juga dapat dipengaruhi oleh beberapa hal, seperti ukuran meristem tip (Verbeek et al, 1995;Ashnayi et al, 2012;Hu et al, 2012), konsentrasi virus di dalam jaringan tanaman (Pramesh dan Baranwal, 2015), genotipe tanaman, jenis ZPT yang digunakan pada kultur in vitro (Ashnayi et al, 2012) dan metode eliminasi (Bhojwani dan Dantu, 2013 …”

Section: Deteksi Virusunclassified

Eliminasi Onion yellow dwarf virus melalui Kultur Meristem Tip pada Bawang Merah (Allium ascalonicum L.)

Aqlima¹,

Purwoko²,

Hidayat³

et al. 2017

J Hort Indonesia

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ABSTRAKKultur meristem tip merupakan kultur meristem yang diisolasi 1-2 primordia daun dan pada media yang sesuai. Metode ini umum digunakan untuk mendapatkan tanaman bebas virus. Optimasi terhadap zat pengatur tumbuh (ZPT) dilakukan untuk mempercepat pertumbuhan eksplan tanpa disertai pembentukan kalus untuk menghindari terjadinya variasi somaklonal pada kultur meristem tip. Penelitian ini bertujuan untuk mendapatkan kombinasi ZPT terbaik bagi pertumbuhan meristem tip dan untuk mengevaluasi potensi kultur meristem tip dalam mengeliminasi virus Onion yellow dwarf virus (OYDV) pada tanaman bawang merah. Penelitian ini menggunakan 0.25 mg L -1 (2-ip, BAP, GA 3 , kinetin) dengan penambahan atau tanpa 0.1 mg L -1 IAA serta media tanpa ZPT. Penelitian ini terdiri atas 2 percobaan terpisah. Percobaan 1 menggunakan cv. Bima Brebes dan Percobaan 2 menggunakan cv. Tiron. Masing-masing percobaan disusun berdasarkan rancangan kelompok lengkap teracak (RKLT) dengan 1 faktor, yaitu kombinasi ZPT yang terdiri atas 8 taraf kombinasi dan 3 ulangan. Hasil yang didapat menunjukkan bahwa media tanpa penambahan ZPT merupakan media yang paling efisien untuk pertumbuhan tunas meristem tip. Tunas utama tumbuh tanpa disertai pembentukan kalus. Hasil analisis RT-PCR menunjukkan bahwa seluruh sampel yang dideteksi masih terinfeksi OYDV. Metode kultur meristem tip belum dapat mengeliminasi virus OYDV pada kedua kultivar bawang merah.

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Efficacy of virus elimination from in vitro-cultured sand pear (Pyrus pyrifolia) by chemotherapy combined with thermotherapy

Cited by 43 publications

References 41 publications

Virus elimination from in vitro apple by thermotherapy combined with chemotherapy

Virus elimination from in vitro apple by thermotherapy combined with chemotherapy

Aplikasi Teknik Embriogenesis Somatik dan Krioterapi untuk Eliminasi Virus SCSMV pada Tanaman Tebu

Eliminasi Onion yellow dwarf virus melalui Kultur Meristem Tip pada Bawang Merah (Allium ascalonicum L.)

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