Increasing fuel prices lead to developing an alternative renewable fuel for internal combustion engines. Pine oil possesses lower viscosity, boiling point and flash point, similar to other plant based fuels like ethanol and eucalyptus oil. Pine oil has been identified to contain terpineol, a higher alcohol, along with pinene, which is an alicyclic hydrocarbon. In this research work, the various performance and emission characteristics of a single cylinder direct injection diesel engine were studied by using an alternative fuel of the biofuel mixed with pine oil and its blends. The results were compared with standard diesel fuel. From the results, it was found that there is a slight increase in brake thermal efficiency and a decrease in brake specific fuel consumption in all pine blended fuels when compared with regular diesel fuel. The use of pine oil resulted in lower emissions of carbon monoxide, hydrocarbons and increased emissions of nitrogen oxides.