Efficiency improvements on ninhydrin method for amino acid quantification

Sun, Shih-Wen; Lin, Yi-Cheng; Weng, Yih‐Ming; Chen, Min-Jane

doi:10.1016/j.jfca.2005.04.006

Cited by 228 publications

(130 citation statements)

References 17 publications

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“…Enzyme activity was determined by measuring the released amino acids using the ninhydrin procedure. The preparation of the ninhydrin reagent and the ninhydrin assay were performed as described by Sun et al (28). Finally, the absorbance at 570 nm was measured by SmartSpect Plus (Bio-Rad, USA), and 1 unit of enzyme activity was defined as mol of released amino acids/min.…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization of the Carboxypeptidase B1 of Anopheles stephensi and Its Evaluation as a Target for Transmission-Blocking Vaccines

Djadid

Zakeri

2013

Infect Immun

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Malaria is one of the most important infectious diseases in the world, and it has many economic and social impacts on populations, especially in poor countries. Transmission-blocking vaccines (TBVs) are valuable tools for malaria eradication. A study on Anopheles gambiae revealed that polyclonal antibodies to carboxypeptidase B1 of A. gambiae can block sexual parasite development in the mosquito midgut. Hence, it was introduced as a TBV target in regions where A. gambiae is the main malaria vector. However, in Iran and neighboring countries as far as China, the main malaria vector is Anopheles stephensi. Also, the genome of this organism has not been sequenced yet. Therefore, in this study, carboxypeptidase B1 of A. stephensi was characterized by genomic and proteomic approaches. Furthermore, its expression pattern after ingestion of Plasmodium falciparum gametocytes and the effect of anti-CPBAs1 antibodies on sexual parasite development were evaluated. Our results revealed that the cpbAs1 expression level was increased after ingestion of the mature gametocytes of P. falciparum and that anti-CPBAs1 directed antibodies could significantly reduce the mosquito infection rate in the test group compared with the control group. Therefore, according to our findings and with respect to the high similarity of carboxypeptidase enzymes between the two main malaria vectors in Africa (A. gambiae) and Asia (A. stephensi) and the presence of other sympatric vectors, CPBAs1 could be introduced as a TBV candidate in regions where A. stephensi is the main malaria vector, and this will broaden the scope for the potential wider application of CPBAs1 antigen homologs/orthologs.

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Section: Methodsmentioning

confidence: 99%

Molecular Characterization of the Carboxypeptidase B1 of Anopheles stephensi and Its Evaluation as a Target for Transmission-Blocking Vaccines

Djadid

Zakeri

2013

Infect Immun

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“…Amino-acid concentration was determined using a method adapted from previously published ones (Frutos, Torrado, Perez-Lorenzo, & Frutos, 2000;Sun, Lin, Weng, & Chen, 2006). The calibration curve and the samples were prepared at the same time.…”

Section: Determination Of Ph Carbohydrate and Amino Acid Concentrationsmentioning

confidence: 99%

Hydrosols of orange blossom ( Citrus aurantium ), and rose flower ( Rosa damascena and Rosa centifolia ) support the growth of a heterogeneous spoilage microbiota

Labadie¹,

Giniès²,

Guinebretière³

et al. 2015

Food Research International

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“…Moreover, total amino acids from the plant samples were measured following the modified method of Moore et al (1954) [7]. Total lipid content in the treated samples was measured (modified Bligh and Dyer [8]) to evaluate the effect of nanoparticles on the plant cell wall components.…”

Section: Increase In Proteins Amino Acids and Lipidsmentioning

confidence: 99%

Effect Sulfur and ZnO Nanoparticles on Stress Physiology and Plant (Vigna radiata) Nutrition

Patra

Choudhury

Mandal

et al. 2013

Springer Proceedings in Physics

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Phytotoxic and agro beneficial properties of antimicrobially efficient Sulfur nanoparticles (SNPs) and ZnO nanoparticles (ZNPs) were evaluated on Mung (Vigna radiata). Mung seeds were germinated with an increasing concentration gradient of nanoparticles. The extent of phtotoxicity were assessed depending upon the effect of used nanoparticles on physical factors (relative root and shoot length, dry weight and area of leaves), photosynthetic pigments (chlorophyll, carotene and xanthophyll content) and mitochondrial stress indicator level (thiol), A simultaneous study was undertaken to understand the effect of SNPs and ZNPs on overall plant growth and nutrition. The nutritive values of nanoparticles were determined in terms of total lipid and protein content. All the aforementioned biochemical assays were performed in triplicate for statistical viability.

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Efficiency improvements on ninhydrin method for amino acid quantification

Cited by 228 publications

References 17 publications

Molecular Characterization of the Carboxypeptidase B1 of Anopheles stephensi and Its Evaluation as a Target for Transmission-Blocking Vaccines

Molecular Characterization of the Carboxypeptidase B1 of Anopheles stephensi and Its Evaluation as a Target for Transmission-Blocking Vaccines

Hydrosols of orange blossom ( Citrus aurantium ), and rose flower ( Rosa damascena and Rosa centifolia ) support the growth of a heterogeneous spoilage microbiota

Effect Sulfur and ZnO Nanoparticles on Stress Physiology and Plant (Vigna radiata) Nutrition

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