Efficiency of different decalcification protocols for nasal osseous structures in a rat experimental model of allergic rhinitis, and their effects on epithelial histology: An attempt at standardization

Guibas, George V.; Lakis, Sotiris; Gkimpas, Christoforos; Manda, Marianthi; Καπουκρανίδου, Δωροθέα; Spandou, Evangelia

doi:10.1016/j.etp.2014.09.001

Cited by 8 publications

(4 citation statements)

References 19 publications

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“…There are limited studies showing the effects of decalcification in surrounding soft tissues. Guibas et al () evaluated the preservation of nasal epithelial histologic structures in rat models after decalcification of nasal structures with formic acid 10%, Morsés solution and EDTA. Short decalcification regimens (5 days) in the formic acid‐based agents and the EDTA (20 days) caused no appreciable damage to the epithelium.…”

Section: Discussionmentioning

confidence: 99%

Tissue integrity, costs and time associated with different agents for histological bone preparation

Abrantes

Rafacho

Rivero

et al. 2016

Microscopy Res & Technique

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The selection of an appropriate demineralizing solution in pathology laboratories depends on several factors such as the preservation of cellularity, urgency of diagnostic and financial costs. The aim of this study was to test different decalcification bone procedures in order to establish the best value of these in formalin-fixed and paraffin-embedded samples. Femurs were removed from 13 adult male Wistar rats to obtain 130 bone disks randomly divided into five groups that were demineralized in different concentrations of nitric acid (Group I); formic acid (Group II); acetic acid (Group III); EDTA, pH7.4 (Group IV) and Morsés solution (Group V). Serial, 3-μm-thick sections were obtained and stained with hematoxylin-eosin to calculate the percentage of osteocyte-occupied lacunae. The sections were also stained with Masson's trichrome in conjunction with picrosirius red under polarized light followed by a semi-quantitative analysis to verify the adjacent muscle-to-bone integrity and preservation of collagen fibres. The highest percentage of osteocyte-occupied lacunae was found with 10% acetic acid solution (95.64 ± 0.95%) and Group I (nitric acid) demanded the shorter time (0.8-5.7days). Of all solutions, 5% nitric acid incurred the lowest cost to achieve complete demineralization compared with other solutions (p < .001). Group IV (EDTA) had the highest integrity of muscle and collagen type I and III (P < 0.01). Demineralization with 10% acetic acid was the most effective at preserving bone tissue, while 5% EDTA was the best at maintaining collagen and adjacent muscle to bone. In conclusion, nitric acid at 5% showed the most efficient result as it balanced both time and cost as a demineralizing solution.

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Section: Discussionmentioning

confidence: 99%

Tissue integrity, costs and time associated with different agents for histological bone preparation

Abrantes

Rafacho

Rivero

et al. 2016

Microscopy Res & Technique

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“…In addition frontal plane sections were obtained from the quarters (Fig f). The blocks consisted of hoof, soft tissue and bone, and excess hard horn was trimmed from the 20 mm × 20 mm × 5 mm blocks before 21 days treatment in Gooding and Stewart's formic acid decalcifying solution to facilitate sectioning of bone . This was followed by alcohol dehydration, paraffin wax embedding, sectioning at 5–7 μm and staining with haematoxylin and eosin, Jones’ periodic acid silver methenamine (PASM) or Masson's trichrome stains and examined via light microscopy (Olympus BX‐50).…”

Section: Methodsmentioning

confidence: 99%

The suspensory apparatus of the distal phalanx in normal horses

Pollitt

Collins

2015

Equine Veterinary Journal

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Sectioning along functional axes demonstrated the true suspensory nature of the SADP connecting the parietal surface to the lamellar hoof wall. SADP/distal phalanx insertions showed penetrating fibres extending through the chondral-apophyseal interface up to and between distal phalanx osteons. Lamellar measurements made from sections perpendicular to the dorsal aspect of the distal phalanx are underestimations but if made along the longer, functional midline 70° transverse plane would accurately reflect the suspensory function of the lamellae. Laminitis pathophysiology correctly viewed as SADP degradation should inform logical, future therapeutic strategies.

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“…Experimental mice were sacrificed after the final OVA challenge, and the heads were kept in 10% formaldehyde solution. Decalcification was performed by previously described protocol [ 34 ]. The specimens were embedded in paraffin, and nasal sections (4 μm) were stained with hematoxylin and eosin (H&E) or toluidine blue to assess inflammatory cell infiltration.…”

Section: Methodsmentioning

confidence: 99%

Impact of Histone H1 on the Progression of Allergic Rhinitis and Its Suppression by Neutralizing Antibody in Mice

et al. 2016

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Nuclear antigens are known to trigger off innate and adaptive immune responses. Recent studies have found that the complex of nucleic acids and core histones that are derived from damaged cells may regulate allergic responses. However, no fundamental study has been performed concerning the role of linker histone H1 in mast cell-mediated type I hyperreactivity. In this study, we explored the impact of histone H1 on mast cell-mediated allergic responses both in vitro and in vivo. In the course of a bona-fide experimental allergen sensitization model upon co-injection with alum adjuvant, ovalbumin (OVA), but not PBS, induced elevated levels of circulating histone H1. Intranasal challenge with histone H1 to OVA/alum- (but not PBS/alum)-sensitized mice induced significantly severer symptoms of allergic rhinitis than those in mice sensitized and challenged with OVA. A monoclonal antibody against histone H1 not only suppressed mast cell degranulation, but also ameliorated OVA-induced nasal hyperreactivity and IgE-mediated passive cutaneous anaphylaxis. Our present data suggest that nuclear histone H1 represents an alarmin-like endogenous mediator acting on mast cells, and that its blockage has a therapeutic potential for mast cell-mediated type I hyperreactivity.

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Efficiency of different decalcification protocols for nasal osseous structures in a rat experimental model of allergic rhinitis, and their effects on epithelial histology: An attempt at standardization

Cited by 8 publications

References 19 publications

Tissue integrity, costs and time associated with different agents for histological bone preparation

Tissue integrity, costs and time associated with different agents for histological bone preparation

The suspensory apparatus of the distal phalanx in normal horses

Impact of Histone H1 on the Progression of Allergic Rhinitis and Its Suppression by Neutralizing Antibody in Mice

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