Efficiency of ESI and APCI ionization sources in LC-MS/MS systems for analysis of 22 pesticide residues in food matrix

Silva, Renata De O.; Menezes, Marília G.G. De; Castro, Rubens Carius de; Nobre, Crisiana de Andrade; Milhome, Maria Aparecida Liberato; Nascimento, Ronaldo F.

doi:10.1016/j.foodchem.2019.06.001

Cited by 35 publications

(13 citation statements)

References 26 publications

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“…Several methodologies have been developed to analyse and detect OPs in specific matrices, considering OPs properties and toxicological effects. Traditional techniques include gas chromatography (GC) or liquid chromatography (LC) coupled to mass spectrometry (MS) [10][11][12][13][14][15], which present the advantage of achieving very high sensitivity and selectivity, allowing to obtain accurate OPs determination. Nevertheless, these methodologies are time-consuming and costly, require pretreatment processes and the need of skilled professionals, and are not suitable for direct and continuous monitoring of pesticides in the field.…”

Section: Introductionmentioning

confidence: 99%

A 3D printable adapter for solid-state fluorescence measurements: the case of an immobilized enzymatic bioreceptor for organophosphate pesticides detection

et al. 2022

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The widespread use of pesticides in the last decades and their accumulation into the environment gave rise to major environmental and human health concerns. To address this topic, the scientific community pointed out the need to develop methodologies to detect and measure the presence of pesticides in different matrices. Biosensors have been recently explored as fast, easy, and sensitive methods for direct organophosphate pesticides monitoring. Thus, the present work aimed at designing and testing a 3D printed adapter useful on different equipment, and a membrane support to immobilize the esterase-2 from Alicyclobacillus acidocaldarius (EST2) bioreceptor. The latter is labelled with the IAEDANS, a bright fluorescent probe. EST2 was selected since it shows a high specificity toward paraoxon. Our results showed good stability and replicability, with an increasing linear fluorescent intensity recorded from 15 to 150 pmol of labelled EST2. Linearity of data was also observed when using the immobilized labelled EST2 to detect increasing amounts of paraoxon, with a limit of detection (LOD) of 0.09 pmol. This LOD value reveals the high sensitivity of our membrane support when mounted on the 3D adapter, comparable to modern methods using robotic workstations. Notably, the use of an independent support significantly simplified the manipulation of the membrane during experimental procedures and enabled it to match the specificities of different systems. In sum, this work emphasizes the advantages of using 3D printed accessories adapted to respond to the newest research needs. Graphical abstract

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Section: Introductionmentioning

confidence: 99%

A 3D printable adapter for solid-state fluorescence measurements: the case of an immobilized enzymatic bioreceptor for organophosphate pesticides detection

et al. 2022

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“… 8 Finally, it enables simultaneous detection and quantification of emerging contaminants in environmental samples and provides efficient ionization efficiency. 5 , 7 As the use of GC-APCI-HRMS gains popularity among scientific communities, 6 , 7 , 9 − 11 there is a demand for developments in in silico structural annotation tools, ionization efficiency scale, and analytical method developments as well as semiquantitative or quantitative analysis. Development of a strategy toward an ionization efficiency scale of GC-APCI amendable compounds as well as their semiquantification in real environmental samples would be a breakthrough.…”

Section: Introductionmentioning

confidence: 99%

First Novel Workflow for Semiquantification of Emerging Contaminants in Environmental Samples Analyzed by Gas Chromatography–Atmospheric Pressure Chemical Ionization–Quadrupole Time of Flight–Mass Spectrometry

et al. 2022

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The ionization efficiency of emerging contaminants was modeled for the first time in gas chromatography-high-resolution mass spectrometry (GC-HRMS) which is coupled to an atmospheric pressure chemical ionization source (APCI). The recent chemical space has been expanded in environmental samples such as soil, indoor dust, and sediments thanks to recent use of high-resolution mass spectrometric techniques; however, many of these chemicals have remained unquantified. Chemical exposure in dust can pose potential risk to human health, and semiquantitative analysis is potentially of need to semiquantify these newly identified substances and assist with their risk assessment and environmental fate. In this study, a rigorously tested semiquantification workflow was proposed based on GC-APCI-HRMS ionization efficiency measurements of 78 emerging contaminants. The mechanism of ionization of compounds in the APCI source was discussed via a simple connectivity index and topological structure. The quantitative structure–property relationship (QSPR)-based model was also built to predict the APCI ionization efficiencies of unknowns and later use it for their quantification analyses. The proposed semiquantification method could be transferred into the household indoor dust sample matrix, and it could include the effect of recovery and matrix in the predictions of actual concentrations of analytes. A suspect compound, which falls inside the application domain of the tool, can be semiquantified by an online web application, free of access at .

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“…Exposure to pesticide residues has been proved to induce toxic effects to unwanted species, non‐target organisms, and human health (Li and Fantke, 2022 ), raising concern about food safety‐related issues. Pesticide monitoring has been achieved due to the use of modern state‐of‐the‐art techniques such as gas chromatography and liquid chromatography coupled to mass spectrometry (GC/LC–MS) (De O. Silva et al, 2019 ; Song et al, 2019 ; Barbieri et al, 2020 ). However, these methodologies are not adequate for real‐time pesticides monitoring, since they are time‐consuming and require highly skilled personnel.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of pesticide amount in water and drinkable food by a fluorescence‐based biosensor

Barbieri

Rodrigues

Febbraio

2022

EFS2

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The identification of pollutants is crucial to protect water resources and ensure food safety. The available analytical methodologies allow reliable detection of organic pollutants such as pesticides; however, there is the need for faster, direct and continuous methodologies for real‐time monitoring of pesticides. Fluorescent‐based biosensors have been recently proposed as a valid alternative due to their advantage of being easy, cheap and specific. In this context, the aim of the present EU‐FORA fellowship programme was to develop and apply a fluorescence‐based biosensing device for the detection of organophosphate (OP) pesticides in water samples and drinkable food. The study was addressed using a mutant of the thermostable esterase‐2 from Alicyclobacillus acidocaldarius (EST2‐S35C) as a bioreceptor for OP pesticides. The use of EST2 involves some significant advantages including specificity and affinity towards OPs, and high stability over time in a different range of temperatures and pH. The protein was labelled to the fluorescent probe IAEDANS and fluorescence measurements of quenching in solution and in immobilised form were performed. The results showed good stability and sensitivity, reaching low limits of detection and quantification and a constant signal intensity over time. The addition of paraoxon quenched the fluorescence of the complex, reaching a plateau at 100 pmol paraoxon. The decrease of enzymatic activity of EST2‐S35C‐IAEDANS in the presence of paraoxon correlated the inhibition of the labelled enzyme with the decrease in fluorescence. The results from the application of the biosensor with real samples showed a decrease in fluorescence in surface water samples, contaminated by OPs. The use of the developed fluorescence‐based biosensor demonstrated its applicability for real samples monitoring and could ensure the production of large amounts of data in a short period of time which can be used to address environmental and food safety risk assessment.

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Efficiency of ESI and APCI ionization sources in LC-MS/MS systems for analysis of 22 pesticide residues in food matrix

Cited by 35 publications

References 26 publications

A 3D printable adapter for solid-state fluorescence measurements: the case of an immobilized enzymatic bioreceptor for organophosphate pesticides detection

A 3D printable adapter for solid-state fluorescence measurements: the case of an immobilized enzymatic bioreceptor for organophosphate pesticides detection

First Novel Workflow for Semiquantification of Emerging Contaminants in Environmental Samples Analyzed by Gas Chromatography–Atmospheric Pressure Chemical Ionization–Quadrupole Time of Flight–Mass Spectrometry

Monitoring of pesticide amount in water and drinkable food by a fluorescence‐based biosensor

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