Efficiency of RAPD and ISSR markers in assessing genetic diversity and relationships in black gram (Vigna mungo L. Hepper) vari

Abstract

, W. 2010. Efficiency of RAPD and ISSR markers in assessing genetic diversity and relationships in black gram (Vigna mungo L. Hepper) varieties. Can. J. Plant Sci. 90: 443Á452. The DNA fingerprinting methodologies, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR), were used to estimate genetic diversity and relationships among 20 black gram (Vigna mungo L. Hepper) varieties. Thirty selected RAPD primers amplified 255 bands, 168 of which were polymorphic (66.5%). On average, these primers produced 8.5 bands, 5.6 of which were polymorphic. Polymorphic band number varied from 2 (A-05) to 10 (OPA-02), with sizes ranging from 100 to 2550 bp. Twenty-four selected ISSR primers produced 238 amplified products, 184 of which were polymorphic (77.8%). On average, these primers generated 9.8 bands, with 7.7 polymorphic bands ranging in number from 4 (ISSR-13) to 11 (ISSR-03), and size from 100Á2650 bp. Genetic relationships were estimated using similarity coefficient (Jaccard's) values between different accession pairs; these varied from 30.7 to 85.0 for RAPD, and from 37.2 to 88.4 with ISSR. UPGMA analysis indicated that the varieties ranged in similarity from 0.50 to 1.00 (mean of 0.75) for RAPD, and from 0.47 to 1.00 (mean of 0.76) with ISSR. Cluster analysis of RAPD and ISSR results identified three clusters with significant bootstrap values, which revealed greater homology between the varieties. Principal coordinates analysis also supported this conclusion. Among the black gram varieties, WBU-108 and RBU-38 were highly divergent, whereas LBG-648 and LBG-623 were genetically similar. The markers generated by RAPD and ISSR assays can provide practical information for the management of genetic resources and these results will also provide useful information for the molecular classification and breeding of new black gram varieties.

Molecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers

“…The ISSR primers employed in the present study afforded 79.23% polymorphism and, therefore, could be considered very efficient at determining the variability in C. argentea. The level of polymorphism reported herein is similar to values obtained for other leguminous species such as Vigna mungo (Kanimozhi et al, 2009;Karuppanapandian et al, 2010), Arachis hypogaea (Mondal et al, 2009), and Caragana microphylla (Huang et al, 2013), but higher than those reported by Souframanien and Gopalakrishna (2004) for V. mungo (54.5%) and by Muthusamy et al (2008) for V. umbellata (61.78%).…”

Genetic diversity analysis in different genotypes of black gram [Vigna mungo (L.) Hepper] using RAPD marker

“…Although iso-zyme and protein markers are useful for characterizing genetic diversity and identifying hybrids of cultivars (Das and Mukarjee 1995;De Vries 1996), the paucity of isozyme and protein methodologies restrict their usefulness in breeding analysis. Molecular markers have been applied widely in genetic analyses and breeding studies, as well as investigations of genetic diversity and relationships between cultivated species (Karuppanapandian et al, 2010). The Randomly Amplified Polymorphic DNA (RAPD) technique (Williams et al, 1990;Hadrys et al, 1992) amongst the PCR based methods has been most popular because of speed, low cost and the use of minute amount of plant material for analysis.…”

Molecular Characterization of Wheat (Triticum sp.) Genotypes Grown in Humid South Eastern Plain Zone of Rajasthan

“…For proofing of different genotypes, classification of several varieties and diversity estimation in crops like Green Gram, (Karuppanapandian et al, 2006) and Black Gram (Karuppanapandian et al, 2007), RAPD marker technique has been extensively used (Fig. 1-3 and Table 6).…”