Efficiency of Real-Time PCR for 18s rRNA amplification of Sorbus domestica L.

The aim of this study was to compare the suitability of two methods for detecting defatted soybean powder; SYBR Green I Real-time PCR and enzyme-linked immunosorbent assay (ELISA). Analysis of 20 artificially contaminated samples prepared by simple dilution with wheat flour revealed that both techniques were able to detect defatted soybean powder, although there were significant differences between the two methods. Wheat flour contamination with defatted soybean powder was detected in samples 1-5, (0.012 %; 120 mg.kg-1), but not in samples with lower contamination with soybean powder saples 6-20 using SYBR Green I real-time PCR. Samples 1-10 could not be quantified by ELISA as the absorbance values were greater than the detection limit, and while samples 11-20 were measured, only the values of samples 16, 17 and 18 were within the guaranteed quantification range specified by the ELISA kit manufacturer. Defatted soybean powder contamination was detected in samples 19 and 20, but absorbance values were highly similar to those of the negative control sample.

Comparison of the sensitivity of determining soyeabean allergens by ELISA method and SYBR green I

Golian¹,

Belej²,

Židek³

et al. 2013

Morphometric characteristics of sweet chestnut (Castanea sativa Mill.) fruits

Grygorieva¹,

Klymenko²,

Brindza

et al. 2017

Aim of this study was to determine morphometric differences of fruits between selected sweet chestnuts (Castanea sativa Mill.). The 28 genotypes (referred as CS-01 to CS-28) were introduced by seeds from Czech Republic, Carpathians, Kyrgyzstan. Genotypes of sweet chestnut are grow more than 30 years in Forest-Steppe of Ukraine in the M.M. Gryshko National Botanical Garden of NAS of Ukraine. They are well adapted to the climatic and soil conditions. The fruits were collected at the period of their full maturity (September). The population differs in weight, shape, size and color of fruits. Their morphometric parameters were following: weight from 1.70 g (CS-26) to 18.60 g (CS-20), length from 8.07 mm (CS-28) to 33.39 mm (CS-11), width from 16.34 mm (CS-28) to 40.95 mm (CS-11), thickness from 9.02 mm (CS-26) to 28.70 mm (CS-11) and hilum length from 6.62 mm (CS-26) to 31.30 mm (CS-07), hilum width from 6.50 mm (CS-23) to 19.99 mm (CS-07). The shape index of the fruits was found in the range of 0.81 (CS-20) to 0.98 (CS-12). The shape index of the hilum was found in the range of 1.48 (CS-04) to 2.03 (CS-23). The outcome of the research point to the fact that the genepool Ukrainian sweet chestnut is a rich source of genetic diversity and might be used in selection for creation a new genotypes and cultivars.

Morphological characteristics for fruits of aronia mitschurinii A.K. Skvortsov & Maitul.

Vinogradova¹,

Grygorieva²,

Vergun³

et al. 2017

The aim of this study was to determine morphometric characteristics of fruits within some phenotypes of Aronia mitschurinii A.K. Skvortsov & Maitul. Their morphometric parameters were following: weight from 0.75 g (AM-03) to 1.52 g (AM-04), length from 9.46 mm (AM-03) to 12.73 mm (AM-04), diameter from 10.49 mm (AM-03) to 13.73 mm (AM-04), fruits number in the corymb from 11.33 (AM-07) to 20.13 (AM-03), cumulative weight of fruits in the corymb from 10.42 g (AM-07) to 21.73 g (AM-04), volume of fruits from 0.55 (AM-03) to 1.26 (AM-04) cm3. The shape index of the fruits was found in the range of 0.87 (AR-01, AR-05, AR-07) to 0.93 (AM-02). The analysis of coefficient of variation showed the difference of variability in morphological characteristics between Aronia mitschurinii samples. Data showed that the most variability of important selection characteristics are the average cumulative mass of fruits in a сorymb - from 12.34 (AM-03) to 38.61 (AM-02) % and fruit number of fruits in the сorymb - from 14.56 (AM-03) to 36.88 (AM-02) %. The other characteristics are more or less stable. The introduction population of the Aronia mitschurinii, was created in the M.M. Gryshko National Botanical Garden in Kyiv, has a sufficient potential for successful selection work.