Efficiency of short, small-diameter columns for reversed-phase liquid chromatography under practical operating conditions

Peak deconvolution to correctly assess the band broadening of chromatographic columns

“…The difference is smaller for the retained components (order of some 0.05-0.2 reduced h-units). This is not unexpected since the relative importance of the system-correction decreases as 2 v,col is proportional with (1 + k) 2 . The fact that the variance difference method consistently leads to higher plate height values, i.e., underestimates the system contribution, was already observed for the theoretical peaks considered in Fig.…”

“…The recent advances in particle and column technology allow to produce columns generating such sharp and narrow peaks that it is very difficult to maintain their very high efficiency when passing through the extra-column volumes of the instrument, even when using the most advanced and optimized instrument designs [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. This also puts a serious strain on the ability to measure the "columnonly" efficiency of these columns [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31], especially when using weakly retained components.…”

Core–Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography

“…A smaller ID monolithic silica column clad with glass, 1 mmID, 5 cm long, afforded similar efficiency as advanced particulate columns of the same dimension with 50% less pressure 130 .…”

Semi-micro reversed-phase liquid chromatography for the separation of alkyl benzenes and proteins exploiting methacrylate- and polystyrene-based monolithic columns

“…fused-silica lined stainless-steel tubes. The composition of the studied polymers was previously described in the literature [1][2][3][4][5]9,[15][16][17][18] . Butyl, hexyl, and lauryl methacrylate [1][2][3]9,15,16] were copolymerized with ethylene glycol dimethacrylate and tested for separation of proteins and alkylbenzenes.…”

“…columns, aiming either better ESI‐MS compatibility or lower mobile phase consumption . Small sized columns (1 mm i.d., 50 mm length) can be used in instruments designed to minimize band broadening in extra‐column volumes . The band broadening originated in the piece of tubing downstream the column is important for all compounds, while that that originated upstream affects significantly only the compounds weakly retained in the column .…”