Efficiency of Tris-Based Extender Steridyl for Semen Cryopreservation in Stallions

Nikitkina, Elena; Musidray, Artem; Krutikova, Anna; Anipchenko, Polina; Plemyashov, Kirill; Shiryaev, G.

doi:10.3390/ani10101801

Cited by 11 publications

(9 citation statements)

References 34 publications

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“…The compositions of extenders are important for semen quality and survival, especially after the freezing–thawing process. Semen extenders have usually been composed of electrolytes (Tris, citric acid), sugars (glucose, fructose), cryoprotectants (egg yolk, glycerol) and antibiotics for several years for semen cryopreservation (Gangwar et al, 2018; Nikitkina et al, 2020). Besides these compounds, antioxidants are very important for mammalian semen freezing.…”

Section: Introductionmentioning

confidence: 99%

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

et al. 2022

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The aim of this study was to determine the effects of thymoquinone (TQ), which is the most essential active compound of Nigella sativa, on the spermatological parameters of ram semen during cryopreservation. Ejaculates were collected from five Sonmez rams using an artificial vagina and extended with Tris‐based extender not containing TQ (control, 0 μg/ml TQ) and containing 10, 25, 50 and 100 μg/ml TQ. The extended semen samples were equilibrated in a + 4°C cold cabinet for 2 h. After 2 h, the samples were loaded into 0.25 ml French straws. The straws were frozen by liquid nitrogen vapour and stored in a liquid nitrogen container (−196°C). The frozen straws were thawed in a water bath (37°C for 30 s) and evaluated in terms of motility characteristics, plasma membrane and acrosome integrity, mitochondrial reactive oxygen species levels, lipid peroxidation levels, DNA damage and biochemical alterations (oxidative stress index, malondialdehyde and glutathione). TQ100 had higher total motility (53.59 ± 3.01) and progressive motility (19.84 ± 1.44; not significantly different from TQ25 and TQ50) compared to the control and TQ10 (p ˂ 0.05). According to the results of the analyses on motility characteristics, there were significant differences between the groups in terms of curvilinear velocity (VCL), amplitude of lateral head displacement (ALH) and linearity (LIN; p ˂ 0.05). The highest DNA damage was detected in the control group (p ˂ 0.05). TQ50 had higher plasma membrane and acrosome integrity (59.56 ± 5.92) compared to the control and TQ25 (p < 0.05) but not significantly different from TQ10 and TQ100. The lowest mitochondrial reactive oxygen species levels were detected in TQ50 and TQ100 (p ˂ 0.05). There were no significant differences among the groups in terms of their oxidative stress index, lipid peroxidation, malondialdehyde and glutathione levels (p > 0.05). According to the results, it could be concluded that supplementing 50 or 100 μg/ml TQ to Tris extenders that were used for ram semen cryopreservation showed a positive effect on motility, plasma membrane integrity and acrosome integrity, and it reduced DNA damage and mitochondrial reactive oxygen species levels.

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Section: Introductionmentioning

confidence: 99%

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

et al. 2022

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“…Sperm was diluted at 1:3 (v/v) ratio with lactose-chelate-citrate-yolk (LCCY) medium containing 3.5% glycerin and frozen according to a standard technology (standard operating procedure) used at the ARRIHB and described in detail elsewhere [11,[26][27][28]. Briefly, four-cornered aluminum tubes were used to package the diluted semen.…”

Section: Semen Examinationmentioning

confidence: 99%

Genome-wide association study for frozen-thawed sperm motility in stallions across various horse breeds

et al. 2022

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Objective:The semen quality of stallions including sperm motility is an important target of selection as it has a high level of individual variability. However, effects of the molecular architecture of the genome on the mechanisms of sperm formation and their preservation after thawing have been poorly investigated. Here, we conducted a genome-wide association study (GWAS) for the sperm motility of cryopreserved semen in stallions of various breeds.Methods: Semen samples were collected from the stallions of 23 horse breeds. The following semen characteristics were examined: progressive motility (PM), progressive motility after freezing (FPM), and the difference between PM and FPM. The respective DNA samples from these stallions were genotyped using Axiom™ Equine Genotyping Array. Results:We performed a GWAS search for single nucleotide polymorphism (SNP) markers and potential genes related to motility properties of frozen-thawed semen in the stallions of various breeds.As a result of the GWAS analysis, two SNP markers, rs1141327473 and rs1149048772, were identified that were associated with preservation of the frozen-thawed stallion sperm motility, the relevant putative candidate genes being NME8, OR2AP1 and OR6C4. Potential implications of effects of these genes on sperm motility are herein discussed. Conclusion:The GWAS results enabled us to localize novel SNPs and candidate genes for sperm motility in stallions. Implications of the study for horse breeding and genetics are a better understanding of genomic regions and candidate genes underlying stallion sperm quality, and improvement in horse reproduction and breeding techniques. The identified markers and genes for sperm cryotolerance and the respective genomic regions are promising candidates for further studying the biological processes in the formation and function of the stallion reproductive system.

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“…Andromed ® contains soy lecithin and tris-buffer, while Optixcell ® contains synthetic lecithin (liposome) (Swelum et al 2019). Furthermore, Steridyl ® is a commercial diluent based on tris and sterile egg yolk (Nikitkina et al 2020). Andromed ® composed of buffer (tris-(hydroxymethyl)-aminomethane, citric acid), sugar (fructose), antioxidants, glycerol, water, and four kinds of antibiotics (tylosin tartrate, gentamycin sulfate, spectinomycin, and lincomycin).…”

Section: Introductionmentioning

confidence: 99%

Frozen Semen Quality of Simmental Cattle in Various Commercial Diluents

Komariah

Zuhdi

Tahar

et al. 2023

JIPTHP

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This study determines the effect of different commercial diluents on the quality of frozen Simmental semen. The extraction of fresh semen from 3 Simmental bulls using an artificial vaginal method was performed twice a week. Fresh semen with motility of >70% was further processed to frozen using a one-step dilution method, and the commercial diluents used were Andromed®, Optixcell®, and Steridyl®. The diluted semen was packed into mini-straws, equilibrated, frozen, and stored for further testing. Data were analyzed by analysis of variance (ANOVA), followed by Duncan Multiple Range Test (DMRT) analysis with a 5% confidence level. The results showed that commercial diluents significantly affected motility, viability, intact plasma membrane, abnormalities, and recovery rate of frozen semen (P<0.05). Commercial diluents with different sources of lecithin reported different results on the quality of frozen semen. Steridyl® has a more optimal ability to maintain the quality of frozen semen of Simmental cattle, followed by Optixcell® and Andromed®.

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Efficiency of Tris-Based Extender Steridyl for Semen Cryopreservation in Stallions

Cited by 11 publications

References 34 publications

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

Genome-wide association study for frozen-thawed sperm motility in stallions across various horse breeds

Frozen Semen Quality of Simmental Cattle in Various Commercial Diluents

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