Efficiency of water disinfectants against Legionella pneumophila and Acanthamoeba

Dupuy, Mathieu; Mazoua, Stéphane; Berne, Florence; Bodet, Charles; Garrec, Nathalie; Herbelin, Pascaline; Ménard-Szczebara, Florence; Oberti, Sandrine; Rodier, Marie‐Hélène; Soreau, Sylvie; Héchard, Yann

doi:10.1016/j.watres.2010.10.025

Cited by 110 publications

(109 citation statements)

References 29 publications

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“…For example, a residual of 1 mg of chlorine/liter is usually found, suggesting that 2 min of contact should be enough to inactivate A. castellanii. Also, the highest Ct 99.9% found with monochloramine is in accordance with most studies, although we have recently shown that this could be dependent of the target strain (10).…”

Section: Discussionsupporting

confidence: 92%

“…Also, monochloramine treatment at the highest doses seemed to greatly modify the cellular membrane morphology. We have previously proposed that monochloramine should have a different mode of action compared to chlorine and chlorine dioxide on the basis of inactivation studies (10). Although further experiments are needed to better understand the mode of action of these oxidants, these results might be helpful to further improve water treatment.…”

Section: Discussionmentioning

confidence: 99%

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Cellular Response of the Amoeba Acanthamoeba castellanii to Chlorine, Chlorine Dioxide, and Monochloramine Treatments

Mogoa

Bodet

Morel

et al. 2011

Appl Environ Microbiol

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Acanthamoeba castellanii is a free-living amoebae commonly found in water systems. Free-living amoebae might be pathogenic but are also known to bear phagocytosis-resistant bacteria, protecting these bacteria from water treatments. The mode of action of these treatments is poorly understood, particularly on amoebae. It is important to examine the action of these treatments on amoebae in order to improve them. The cellular response to chlorine, chlorine dioxide, and monochloramine was tested on A. castellanii trophozoites. Doses of disinfectants leading to up to a 3-log reduction were compared by flow cytometry and electron microscopy. Chlorine treatment led to size reduction, permeabilization, and retraction of pseudopods. In addition, treatment with chlorine dioxide led to a vacuolization of the cytoplasm. Monochloramine had a dose-dependent effect. At the highest doses monochloramine treatment resulted in almost no changes in cell size and permeability, as shown by flow cytometry, but the cell surface became smooth and dense, as seen by electron microscopy. We show that these disinfectants globally induced size reduction, membrane permeabilization, and morphological modifications but that they have a different mode of action on A. castellanii.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Cellular Response of the Amoeba Acanthamoeba castellanii to Chlorine, Chlorine Dioxide, and Monochloramine Treatments

Mogoa

Bodet

Morel

et al. 2011

Appl Environ Microbiol

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“…in natural environments Greub and Raoult, 2004Molmeret et al, 2005Rowbotham, 1980Winiecka-Krusnell and Linder, 2001 . In addition, L. pneumophila co-cultured with amoeba is less sensitive to typical chlorine disinfectants Barker et al, 1992Dupuy et al, 2011García et al, 2007Kim et al, 2002 and L. pneumophila multiplied in amoebae possess greater resistance to disinfectants and antibacterial agents than L. pneumophila from cultures in broth Bandyopadhyay et al, 2004Barker et al, 1995 . L. pneumophila in biofilms is also resistant to stressful environmental conditions and the actions of antibacterial agents Cooper and Hanlon, 2010Kim et al, 2002Wright et al, 1991 .…”

Section: Discussionmentioning

confidence: 99%

Effects of Oakmoss and its Components on Acanthamoeba castellanii ATCC 30234 and the Uptake of Legionella pneumophila JCM 7571 (ATCC 33152) into A. castellanii

et al. 2015

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Acanthamoeba castellanii, a ubiquitous organism in water environments, is pathogenic toward humans and also is a host for bacteria of the genus Legionella, a causative agent of legionellosis. Oakmoss, a natural fragrance ingredient, and its components are antibacterial agents specifically against the genus Legionella. In the present study, oakmoss and its components were investigated for their amoebicidal activity against A. castellanii ATCC 30234 and the inhibitory effect on the uptake of L. pneumophila JCM 7571 ATCC 33152 into A. castellanii. The oakmoss and its components 3-hydroxy-5-methylphenyl 2,4-dihydroxy-6-methylbenzoate 5 , and 6,8-dihydroxy-3-pentyl-1H-isochromen-1-one 12 exhibited high amoebicidal activity IC 50 values 10.5 2.3, 16.3 4.0 and 17.5 2.8 µg/mL, respectively after 48 h of treatment, which were equivalent to that of the reference compound, chlorhexidine gluconate. Pretreatment of L. pneumophila with sub-minimal inhibitory concentration of oakmoss, compound 5, 3-hydroxy-5-methylphenyl 2-hydroxy-4-methoxy-6-methylbenzoate 10 and 8-2,4-dihydroxy-6-pentylphenoxy -6-hydroxy-3-pentyl-1H-isochromen-1-one 14 obviously reduced the uptake of L. pneumophila into A. castellanii p 0.05 . The inhibitory effect of compound 5 on the uptake of L. pneumophila was almost equivalent to that of ampicillin used as a reference. Thus, the oakmoss and its components were considered to be good candidates for disinfectants against not only genus Legionella but also A. castellanii.

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“…Reservoir, vector and vehicle (Cirrillo et al, 1997;Harb et al, 2000;Schuster et al, 2002;2004;Dalebroux et al, 2009;Iseberg et al, 2009;Dupuy et al, 2011) T a b l e 2 (continued) Axelsson-Olsson et al, 2005;Snelling et al, 2006;Huws et al, 2008;Thomas et al, 2010;Bui et al, 2012;Siddiqui and Khan, 2012a;Cateau et al, 2014) Vibrio cholerae, V. mimicus, V. parahaemolyticus Intra-amoebal habitat and reservoir (Thom et al, 1992;Laskowski-Arce and Orth, 2008;Matz et al, 2008;Abd et al, 2010;Vezzulli et al, 2010) Yersinia enterocolitica Reservoir, vector, infection route, biological gym, and evolutionary crib (Huws et al, 2008) E. coli, Salmonella spp. Mediator (Vaerewijck et al, 2014) Afipia birgiae, A. broomae, A. felis, A. massiliensis Facultative intracellular Balamuthia spp.…”

Section: Legionella Pneumophilamentioning

confidence: 99%

“…Reservoir, vector and vehicle (Cirrillo et al, 1997;Harb et al, 2000;Schuster et al, 2003;Schuster and Visvesvara, 2004;Dalebroux et al, 2009;Iseberg et al, 2009;Dupuy et al, 2011) Simkania negevensis Reservoir (Thomas et al, 2010) Dictyostelium discoideum Mycobacterium avium, M. marinum Reservoir (Cateau et al, 2014) Vibrio cholerae, V. mimicus, V. parahaemolyticus Intra-amoebal habitat and reservoir (Thom et al, 1992;Laskowski-Arce and Orth, 2008;Abd et al, 2010;Vezzulli et al, 2010;Matz et al, 2011) Pseudomonas aeruginosa Reservoir (Snelling et al, 2006) Entamoeba histolytica Shigella dysenteriae, S. flexneri, S. sonnei Reservoir (transmission reservoir) Saeed et al, 2009) Ehinamoeba spp., Hartmanella spp., Vahlkamphia spp.…”

Section: Legionella Pneumophilamentioning

confidence: 99%

Relationships between Free-Living Amoeba and their Intracellular Bacteria

Rubeniņa

Kirjušina

Bērziņš

et al. 2017

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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An increasing number of bacteria have been described as benefiting from interaction with free-living amoeba. The most common association between free-living amoeba and microorganisms is interaction of various non-pathogenic and pathogenic bacterial species with amoeba. Various pathogenic bacterial species have capacity to resist digestion by free-living amoeba, which has been observed by many researchers. Also, several of these pathogens are able to resist digestion by macrophages. In addition, free-living amoeba have been associated with several diseases in humans. Acanthamniioeba castella is an important predator of bacteria. It is a ubiquitous organism in water, soil, and air. Attention from a public health perspective is needed by investigation of interaction of foodborne pathogens and free-living amoeba. Bacteria can use free-living amoeba as reservoirs, mediators or vehicles, an infection route, “biological gym” and evolutionary crib or interaction may result in a close endosymbiotic relationship. The purpose of this review is to describe the interaction mechanisms between free-living amoeba and common bacteria species that survive in host cells.

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Efficiency of water disinfectants against Legionella pneumophila and Acanthamoeba

Cited by 110 publications

References 29 publications

Cellular Response of the Amoeba Acanthamoeba castellanii to Chlorine, Chlorine Dioxide, and Monochloramine Treatments

Cellular Response of the Amoeba Acanthamoeba castellanii to Chlorine, Chlorine Dioxide, and Monochloramine Treatments

Effects of Oakmoss and its Components on <i>Acanthamoeba</i><i> castellanii</i> ATCC 30234 and the Uptake of <i>Legionella pneu</i><i>mophila</i> JCM 7571 (ATCC 33152) into <i><i>A. castellanii</i></i>

Relationships between Free-Living Amoeba and their Intracellular Bacteria

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