Efficiency of Winter Wheat Seed Inoculation by the Bacillus Bacteria Promising for the Development of Bio Medicines

Abstract

As a result of stage screening, there were selected promising bacterial strains to create laboratory samples of biological products on their basis to protect winter wheat from the causative agents of fusarium root rot. The purpose of the work is to determine the growth-stimulating and protective effect of laboratory samples of Bacillus subtilis BZR 336g and B. subtilis BZR 517 on winter wheat plants, as well as to determine the antagonistic effect of strains on Fusarium fungi. As a result of the work, there was established a positive effect of laboratory samples on the growth, development and germination of plants in greenhouse conditions and the ability to significantly inhibit the mycelium of phytopathogenic fungi F. graminearun, F. culmorum and Microdochium nivale in laboratory conditions. The study of the samples artificially infected in a climatic chamber showed not only a high protective effect of these laboratory samples, but also made it possible to determine the regimes for their use. The conducted tests prove the viability of new bioagents as environmentally friendly plant-protective products.

Draft Genome Sequence of Bacillus velezensis BZR 336g, a Plant Growth-Promoting Antifungal Biocontrol Agent Isolated from Winter Wheat

“…The BZR 336g strain was originally isolated from a winter wheat rhizoplane, and the plant source was grown in the Krylovsky district of the Krasnodar region in Russia. The strain was described previously as Bacillus subtilis BZR 336g (according to phenotyping and 16S rRNA gene analysis data) ( 6 ) and was placed in the Departmental Collection of Useful Microorganisms for Agricultural Purposes (accession number RCAM01729) as a highly effective producer of hydrolytic protease and lipase ferments and as a suppressor of common crop phytopathogens, such as Fusarium graminearum , Fusarium culmorum , Microdochium nivale , and Pyrenophora tritici-repentis ( 7 , 8 ). The BZR 336g strain has demonstrated commercial promise as a biological agent ( 9 ) for crop pest control ( 10 ).…”

Draft Genome Sequence of the Plant Growth-Promoting Bacterium Bacillus subtilis Strain BZR 517, Isolated from Winter Wheat, Now Reclassified as Bacillus velezensis Strain BZR 517

“…(8,9), with an absence of pathogenic properties for plants and homeothermic animals. It was also patented as a prospective biological agent for crop protection and control of phytopathogenic fungi (10,11).…”