Efficient Bioconversion of Sucrose to High‐Value‐Added Glucaric Acid by In Vitro Metabolic Engineering

Su, Huihui; Guo, Zewang; Wu, Xiaoling; Xu, Pei; Li, Ning; Zong, Min‐Hua; Lou, Wen‐Yong

doi:10.1002/cssc.201900185

Cited by 29 publications

(29 citation statements)

References 38 publications

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“…The synthesis of rare sugars like β(1,4)-galactosyl l-rhamnose from sucrose can be enabled in that way (Figure 3c) [35][36][37]. Obviously, other biocatalysts beyond phosphorylases can be employed downstream of SP too, as was demonstrated in the production of glucaric acid from sucrose in a seven-enzyme cascade [38]. Furthermore, the atom efficiency of certain cascade processes can be boosted by added auxiliary enzymes that convert side products back into substrates [37].…”

Section: Synthesis Of Phosphorylated Sugarsmentioning

confidence: 99%

Sucrose Phosphorylase and Related Enzymes in Glycoside Hydrolase Family 13: Discovery, Application and Engineering

Franceus

Desmet

2020

IJMS

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Sucrose phosphorylases are carbohydrate-active enzymes with outstanding potential for the biocatalytic conversion of common table sugar into products with attractive properties. They belong to the glycoside hydrolase family GH13, where they are found in subfamily 18. In bacteria, these enzymes catalyse the phosphorolysis of sucrose to yield α-glucose 1-phosphate and fructose. However, sucrose phosphorylases can also be applied as versatile transglucosylases for the synthesis of valuable glycosides and sugars because their broad promiscuity allows them to transfer the glucosyl group of sucrose to a diverse collection of compounds other than phosphate. Numerous process and enzyme engineering studies have expanded the range of possible applications of sucrose phosphorylases ever further. Moreover, it has recently been discovered that family GH13 also contains a few novel phosphorylases that are specialised in the phosphorolysis of sucrose 6F-phosphate, glucosylglycerol or glucosylglycerate. In this review, we provide an overview of the progress that has been made in our understanding and exploitation of sucrose phosphorylases and related enzymes over the past ten years.

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Section: Synthesis Of Phosphorylated Sugarsmentioning

confidence: 99%

Sucrose Phosphorylase and Related Enzymes in Glycoside Hydrolase Family 13: Discovery, Application and Engineering

Franceus

Desmet

2020

IJMS

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“…They also showed how the system could be used for increasing expression of protein synthesis for the selected gene in C. autoethanogenum, with the possibility of adaptation and optimisation of the prototyping system for improvement of yields by solventogenic and cellulolytic Clostridia. Zhong et al, 2017bSu et al, 2019Tian et al, 2019Tian et al, 2019Jung et al, 2013Zhong and Nidetzky, 2020 (Continued)…”

Section: Prototypingmentioning

confidence: 99%

“…Most glucaric acid is produced by nitric acid oxidation of glucose and attempts at whole cell fermentative production by E. coli and yeast have not been economically viable (Moon et al, 2009;Gupta et al, 2016). A cell-free synthetic pathway was described by Su et al (2019) that used seven enzymes in a one-pot reaction at a moderate temperature in the production of glucaric acid from 50 mM sucrose, producing 35 mM glucaric acid in 70 h (75% molar yield) ( Figure 3A). The kinetic parameters of the rate-limiting enzyme, myo-inositol oxygenase (MIOX) were compared from various biological sources and the one from Arabidopsis thaliana was reported to have the highest specific activity.…”

Section: Cell-free Enzyme Pathwaysmentioning

confidence: 99%

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Cell-Free Biocatalysis for the Production of Platform Chemicals

2020

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Genetically engineered host bacteria have an extensive history for the production of specific proteins including the synthesis of single enzymes for the modification of compounds produced for industrial purposes by biological or chemical processes. Such processes have been developed largely through the process of discovery. The ability to assemble multiple enzymes into synthetic pathways is a new development aided by the synthetic biology approach of constructing and assembling suitable enzymes into pathways that may or may not occur in Nature to provide a highimpact platform for bio-manufacturing of chemicals, biofuels and pharmaceuticals. Industry has depended on chemical catalysts because of the known constraints experienced frequently with biological catalysts but when high stereochemistry, mild synthesis conditions and environmentally friendly processes are significant, application of enzymes is preferred, especially in the case of drug synthesis where enantioselectivity is important. However, many whole cell production processes are beset by toxicity problems, metabolite competition, the production of side products, sub-optimal enzyme ratios and varying temperature optima. As a result, a cell-free biocatalysis allows the manipulation of substrate ratios, provision of regenerated cofactors and adjustment of high energy flux ratios that are difficult or impossible to control in whole cell synthesis. We discuss here the construction of cell free biocatalytic pathways as added free enzymes or multi-enzyme modules that may contain heterologous catalysts. We examine the status of applications leading to commercialisation, emphasizing the importance of economical cofactor regeneration. Nevertheless, problems remain, particularly protein post-translational modifications including glycosylation, phosphorylation, ubiquitination, acetylation, proteolysis, etc. The National Renewable Energy Laboratory and Pacific North West Laboratory have published lists of top value-added chemicals from biomass that include glucaric acid. There have been few reports on the combination of synthetic biology and cell-free protein synthesis to set up pathways for these valuable intermediate compounds. This observation is despite the existence of at least one large scale but specialized cell-free production of antibody conjugates. This review will provide a description of one successful attempt at the cell-free production of glucaric acid and will evaluate progress for other key intermediate and platform chemicals.

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“…In 2019, an in vitro GA biosynthesis method using sucrose as substrate and seven enzymes was established (Fig. 4 a) [ 64 ]. Briefly, sucrose phosphorylase (SP) converted sucrose into G1P and d -fructose.…”

Section: Cell-free Multi-enzyme Catalysis Approaches To Produce Gamentioning

confidence: 99%

Cell-based and cell-free biocatalysis for the production of d-glucaric acid

Chen

Huang²,

Hou

et al. 2020

Biotechnol Biofuels

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Abstractd-Glucaric acid (GA) is a value-added chemical produced from biomass, and has potential applications as a versatile platform chemical, food additive, metal sequestering agent, and therapeutic agent. Marketed GA is currently produced chemically, but increasing demand is driving the search for eco-friendlier and more efficient production approaches. Cell-based production of GA represents an alternative strategy for GA production. A series of synthetic pathways for GA have been ported into Escherichia coli, Saccharomyces cerevisiae and Pichia pastoris, respectively, and these engineered cells show the ability to synthesize GA de novo. Optimization of the GA metabolic pathways in host cells has leapt forward, and the titer and yield have increased rapidly. Meanwhile, cell-free multi-enzyme catalysis, in which the desired pathway is constructed in vitro from enzymes and cofactors involved in GA biosynthesis, has also realized efficient GA bioconversion. This review presents an overview of studies of the development of cell-based GA production, followed by a brief discussion of potential applications of biosensors that respond to GA in these biosynthesis routes.

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Efficient Bioconversion of Sucrose to High‐Value‐Added Glucaric Acid by In Vitro Metabolic Engineering

Abstract: Supporting Information and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.

Cited by 29 publications

References 38 publications

Sucrose Phosphorylase and Related Enzymes in Glycoside Hydrolase Family 13: Discovery, Application and Engineering

Sucrose Phosphorylase and Related Enzymes in Glycoside Hydrolase Family 13: Discovery, Application and Engineering

Cell-Free Biocatalysis for the Production of Platform Chemicals

Cell-based and cell-free biocatalysis for the production of d-glucaric acid

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