Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from Pseudomonas fluorescens

Bartha-Vári, Judith-Hajnal; Moisă, Mădălina Elena; Bencze, L.; Irimie, Florin Dan; Paizs, Csaba; Toşa, Monica Ioana

doi:10.3390/molecules25030651

Cited by 28 publications

(6 citation statements)

References 48 publications

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“…Different sources of lipases used in microalgae biodiesel production are listed in Table 3. The hydrophobicity of the nanoparticle is a characteristic that helps with both the immobilization of lipase and the synthesis of biodiesel [100]. Because lipases naturally bind to hydrophobic surfaces, their immobilization on nanoparticles is distinct from that of enzymes that adsorb hydrophobically.…”

Section: Enzyme Immobilization and Nanotechnology-driven Microalgae B...mentioning

confidence: 99%

Bio-Nanoparticles Mediated Transesterification of Algal Biomass for Biodiesel Production

Verma,

Dhanya,

Wang

et al. 2023

Sustainability

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Immense use of fossil fuels leads to various environmental issues, including greenhouse gas emissions, reduced oil reserves, increased energy costs, global climate changes, etc. These challenges can be tackled by using alternative renewable fuels such as biodiesel. Many studies reported that biodiesel production from microalgae biomass is an environment-friendly and energy-efficient approach, with significantly improved fuel quality in terms of density, calorific value and viscosity. Biodiesel is produced using the transesterification process and the most sustainable method is utilizing enzymes for transesterification. Lipase is an enzyme with excellent catalytic activity, specificity, enantio-selectivity, compatibility and stability and hence it is applied in microalgae biodiesel production. But, difficulty in enzymatic recovery, high enzyme cost and minimal reaction rate are some of its drawbacks that have to be addressed. In this aspect, the nanotechnological approach of lipase immobilization in producing microalgae biodiesel is a promising way to increase production yield and it is due to the adsorption efficiency, economic benefit, recyclability, crystallinity, durability, stability, environmental friendliness and catalytic performance of the bio-nanoparticles used. Through increasing post-harvest biomass yield, absorption of CO2 and photosynthesis in the photobioreactor, the use of nanoparticle immobilized lipase during the generation of biodiesel from microalgae has the potential to also remove feedstock availability constraints. This review article discusses the production of microalgae biodiesel, and effect of nanoparticles and immobilized lipase nanoparticles on biodiesel production. The advantages of using lipase nanoparticles and the challenges in introducing the immobilized lipase on nanoparticles in large-scale microalgae biodiesel production are also discussed. Reducing the water and land use, energy and nutrient footprints of integrated algae-based operations must be the main goal of larger-scale experiments as well as ongoing research and development in order to expedite the adoption of microalgae-based biodiesel production. Also, the cost-effectiveness and large-scale availability of nanoparticles and the impact of lipase nanoparticles on engine performance should be analyzed for commercialization of microalgae biodiesel.

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Section: Enzyme Immobilization and Nanotechnology-driven Microalgae B...mentioning

confidence: 99%

Bio-Nanoparticles Mediated Transesterification of Algal Biomass for Biodiesel Production

Verma,

Dhanya,

Wang

et al. 2023

Sustainability

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“…The feedstock for biodiesel production contains edible oils, such as soybean and palm oil, as well as non‐edible oils, such as cooking oils and algal or fungal oils . Edible oils are considered as a low‐cost source for biodiesel production compared to the non‐edible oils produced from algae .…”

Section: Introductionmentioning

confidence: 99%

“…[5] The feedstock for biodiesel production contains edible oils, such as soybean and palm oil, as well as non-edible oils, such as cooking oils and algal or fungal oils. [6] Edible oils are considered as a low-cost source for biodiesel production compared to the non-edible oils produced from algae. [7] Triglycerides of edible oils are generally composed of a variety of fatty acids, such as (9Z)-9-octadecenoic acid (oleic acid, OA), (9Z,12Z)-9,12-octadecadienoic acid (linoleic acid, LA), myristic, palmitic and stearic acids.…”

Section: Introductionmentioning

confidence: 99%

Biodiesel Production using Lewis Catalysts under Ultrasonic Activation

Kolet

Zerbib

Molina

et al. 2020

Israel Journal of Chemistry

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Biodiesel can be produced by transesterification of oil triglycerides, as well as by esterification of fatty acids using feedstock materials rich in free fatty acids)FFAs). Utilization of such feedstocks can gain substantial economical profit and solve a problem of disposal of residual raw materials. To find an effective method of biodiesel production from FFA, we developed esterification of FFAs under ultrasonic activation using Lewis acids as catalysts. Homologous catalyst BF3 and heterogeneous AlCl3 were highly effective in reactions of model FFAs methylation under ultrasonic activation, when the reaction yield reached 100 %. Ethylation at the same conditions was less successful.

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“…Biodiesel is a biofuel produced from natural components and can be used as a full or at least partial substitute for fossil fuels. It can be obtained from a variety of animal fats, vegetable oils, cooking oil wastes and wastewater grease [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…Biodiesel is based on organic hydrocarbon alkyl esters that can comprise an eco-friendly alternative to fossil fuels, since it can be obtained from vegetable oils or animal fats [6,7]. Since major plant fatty acids and triacylglycerols are produced in plant cells through de novo synthesis, they are regarded as a renewable oil source [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Production of Biodiesel from Brown Grease

et al. 2020

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Among the renewable energy sources is biodiesel. This fuel is usually produced by catalytic transesterification of vegetable oils and animal fats under heating and pressure. Brown grease is a mixture of oils, fats, solids and detergents from food industry wastes that is captured in grease traps. Brown grease is classified as waste and must be treated and disposed of appropriately. It contains oils and fats that can be converted into biodiesel. However, the high concentration of free fatty acids in brown grease does not enable the use of conventional biodiesel production schemes. This study proposes a new scheme for biodiesel production from brown grease. In addition, conditions for the effective separation of a fat phase from brown grease were tested, and the composition of a fatty phase was determined for several grease traps. Esterification and transesterification of brown grease lipids were carried out with methanol, where the Lewis acids BF3 and AlCl3 were used as catalysts and the reaction was activated by ultrasound. The results show that biodiesel can be obtained from brown grease by esterification and transesterification within several minutes under ultrasonic activation at room temperature. These results open prospects for the development of efficient, low-cost and environmentally friendly biodiesel production.

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Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from Pseudomonas fluorescens

Cited by 28 publications

References 48 publications

Bio-Nanoparticles Mediated Transesterification of Algal Biomass for Biodiesel Production

Bio-Nanoparticles Mediated Transesterification of Algal Biomass for Biodiesel Production

Biodiesel Production using Lewis Catalysts under Ultrasonic Activation

Production of Biodiesel from Brown Grease

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