Efficient biorefinery of whole cassava for citrate production using Aspergillus niger mutated by atmospheric and room temperature plasma and enhanced co‐saccharification strategy

Abstract

BACKGROUND The non‐grain crop cassava has attracted intense attention in the biorefinery process. However, efficient biorefinery of whole cassava is faced with some challenges due to the existence of strain inhibition and refractory cellulose during the citrate production process. RESULTS Here, a novel breeding method – atmospheric and room temperature plasma (ARTP) – was applied for strain improvement of citrate‐producing strain Aspergillus niger from whole cassava. The citrate yield of the mutant obtained using ARTP mutagenesis increased by 36.5% in comparison with the original strain. Moreover, citric acid fermentation was further improved on the basis of an enhanced co‐saccharification strategy by supplementing glucoamylase and cellulase. The fermentation efficiency increased by 35.8% with a 17.0 g L−1 reduction in residual sugar on a pilot scale. CONCLUSIONS All these results confirmed that a combination of the novel breeding method and enhanced co‐saccharification strategy could be used to efficiently refine whole cassava. The results also provide inspiration for the production of value‐added products and waste disposal in agro‐based industries. © 2021 Society of Chemical Industry

ARTP Mutagenesis of Schizochytrium sp. PKU#Mn4 and Clethodim-Based Mutant Screening for Enhanced Docosahexaenoic Acid Accumulation

“…The optimal exposure time (40 s) of ARTP treatment to strain PKU#Mn4 was comparable with that of the microalgae such as Crypthecodinium cohni [39], Spirulina platensis [19], Chlorella pyrenoidosa [43] and Spirulina platensis [44]. However, compared with fungi, e.g., Monascus purpureus LQ-6 [45] and Aspergillus niger [46], the strain PKU#Mn4 was much more sensitive to the exposure time. These reported differences in the treatment duration could be attributed to the differential biochemical features of the microalgal and fungal cell walls that act as a natural barrier against environmental pressures [47].…”