Efficient biosynthesis of<scp>d</scp>-ribose using a novel co-feeding strategy in<i>Bacillus subtilis</i>without acid formation

Cheng, Jian; Zhuang, Wei-Qin; Li, Nana; Tang, Chenglun; Ying, Hanjie

doi:10.1111/lam.12685

Cited by 17 publications

(12 citation statements)

References 13 publications

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“…Normally, Bacillus spp. usually produce acetoin, lactate and acetate as the metabolic products as a consequence of mismatched glycolytic and tricarboxylic acid cycle capacities when glucose is used as carbon source [38,39]. No related works have reported whether acetate can be used as the sole carbon source by Bacillus spp.…”

Section: Effect Of Flavins On Power Generation and Eet Efficiencymentioning

confidence: 99%

Flavins mediate extracellular electron transfer in Gram-positive Bacillus megaterium strain LLD-1

You

Liu

Xiao

et al. 2018

Bioelectrochemistry

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The extracellular electron transfer (EET) mechanism of an isolated Gram-positive Bacillus megaterium strain (LLD-1), identified by 16S rRNA gene sequencing and physiological analysis, was investigated in the present study. The electrochemical activity of strain LLD-1 was confirmed by electrochemical E-t and amperometric I-t tests. Flavins in culture suspension from strain LLD-1 were further proved to be able to act as electron shuttles, strengthening the electron transfer from LLD-1 to the electrode. The output voltage and current output were increased 2.8 times and 3.7 times, respectively, by adding 100nM exogenetic flavins into microbial fuel cells inoculated with LLD-1. Electricity generation by LLD-1 from different carbon sources can be enhanced by adding 100nM exogenetic flavins. This study indicated that flavins were essential to the EET process of the Gram-positive strain LLD-1. Furthermore, a putative EET model for B. megaterium strain LLD-1 and even for Gram-positive bacteria was proposed.

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Section: Effect Of Flavins On Power Generation and Eet Efficiencymentioning

confidence: 99%

Flavins mediate extracellular electron transfer in Gram-positive Bacillus megaterium strain LLD-1

You

Liu

Xiao

et al. 2018

Bioelectrochemistry

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“…developed different mechanisms to enhance plant growth by increasing availability of the nutrient [ 5 ]. One of the main mechanisms recognized as responsible for releasing available forms of phosphorus to plants in the soil is the production of organic acids (solubilization of insoluble inorganic phosphate compounds such as tricalcium phosphate, dicalcium phosphate, hydroxyapatite, and rock phosphate) [ 6 , 7 ] and the increase in activity of acid phosphatases (mineralization of organic phosphorous) [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Phosphorus Solubilization by Bacillus Species

2018

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Microbial solubilization applies the natural ability of a microorganism to liberate phosphorus from unavailable structures. The main mechanism recognized to be responsible for the solubilization of phosphorus is the production of different types of organic acids. Three kinds of Bacillus species and three types of raw materials (poultry bones, fish bones, and ash) were tested for solubilization. The following parameters were compared for all discussed cases: pH, specific growth rate, solubilization factor, released phosphorus concentration, and total and individual concentration of organic acids. Utilization of ash brought about the highest specific and maximum specific growth rates. A decrease in pH was observed in most of the discussed cases with the exception of fish bones. At the same time, fish bones had the highest concentration of released P2O5 and the highest total concentration of produced organic acids (gluconic, lactic, acetic, succinic, and propionic) in all discussed cases. The tested Bacillus species produced the mentioned acids with the exception of B. megaterium, where propionic acid was not present. The lactic and acetic acids were those produced in the highest amount. The kind of raw materials and type of Bacillus species used in solubilization had a strong influence on the kind of organic acids that were detected in the broth culture and its total concentration, which had a direct influence on the amount of released phosphorus. The combination of Bacillus megaterium with the fish bones at 5 g/L is proposed as the pair that gives the highest concentration of released phosphorus (483 ± 5 mg/L).

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“…Long before the term chassis microorganism was introduced to the literature, Bacillus subtilis was already applied in different fields. Today, B. subtilis strains excrete the fine chemical D‐ribose to concentrations of more than 100 g per litre (Cheng et␣al ., 2017). The production of the high‐value polysaccharide hyaluronic acid by a membrane‐associated enzyme heterologous (Westbrook et␣al ., 2018) is also economically competitive.…”

Section: Introductionmentioning

confidence: 99%

Aspergillus fumigatus AR04 obeys Arrhenius' rule in cultivation temperature shifts from 30 to 40°C

Nieland

Barig

Gehrau

et al. 2021

Microbial Biotechnology

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To set a benchmark in fungal growth rate, a differential analysis of prototrophic Aspergillus fumigatus AR04 with three ascomycetes applied in > 10 3 t year -1 scale was performed, i.e. Ashbya gosspyii (riboflavin), Aspergillus niger (citric acid) and Aspergillus oryzae (food-processing). While radial colony growth decreased 0.5-fold when A. gossypii was cultivated at 40°C instead of 28°C, A. fumigatus AR04 responded with 1.7-fold faster hyphal growth. A. niger and A. oryzae formed colonies at 40°C, but not at 43°C. Moreover, all A. fumigatus strains tested grew even at 49°C. In chemostat experiments, A. fumigatus AR04 reached steady state at a dilution rate of 0.7 h -1 at 40°C, 120% more than reported for A. gossypii at 28°C. To study mycelial growth rates under unlimited conditions, carbon dioxide increase rates were calculated from concentrations detected online in the exhaust of batch fermentations for 3 h only. All rates calculated suggest that A. fumigatus AR04 approximates Arrhenius' rule when comparing short cultivations at 30°C with those at 40°C. Linearization of the exponential phase and comparison of the slopes revealed an increase to 192% by the 10°C up-shift.

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Efficient biosynthesis ofd-ribose using a novel co-feeding strategy inBacillus subtiliswithout acid formation

Cited by 17 publications

References 13 publications

Flavins mediate extracellular electron transfer in Gram-positive Bacillus megaterium strain LLD-1

Flavins mediate extracellular electron transfer in Gram-positive Bacillus megaterium strain LLD-1

Phosphorus Solubilization by Bacillus Species

Aspergillus fumigatus AR04 obeys Arrhenius' rule in cultivation temperature shifts from 30 to 40°C

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