Efficient chromatographic reduction of ovalbumin for egg-based influenza virus purification

Blom, Henning; Åkerblom, Anna; Kon, Theone; Shaker, Sabah; Pol, Leo van der; Lundgren, Mats

doi:10.1016/j.vaccine.2014.04.033

Cited by 41 publications

(28 citation statements)

References 10 publications

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“…700 kDa engineered for purification of viruses and other large biomolecules, was used in FT mode chromatography (Blom et al . ). The analysis after equilibration and elution indicated that most of the EV71 VLPs were collected in FT fraction (Fig.…”

Section: Resultsmentioning

confidence: 97%

Enterovirus71 virus-like particles produced from insect cells and purified by multistep chromatography elicit strong humoral immune responses in mice

et al. 2015

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Section: Resultsmentioning

confidence: 97%

Enterovirus71 virus-like particles produced from insect cells and purified by multistep chromatography elicit strong humoral immune responses in mice

et al. 2015

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“…Hence, an RNase treatment was added followed by Capto Core chromatography. Capto Core resin was developed to separate flu virus away from contaminants such as albumin [48,49]. The concentrated CFF/PEG EV solution from BCBL-1 cell culture supernatant was loaded into a Capto Core column and fractions collected for analysis.…”

Section: Resultsmentioning

confidence: 99%

Large‐scale, cross‐flow based isolation of highly pure and endocytosis‐competent extracellular vesicles

McNamara

Caro-Vegas

Costantini

et al. 2018

J of Extracellular Vesicle

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Isolation of extracellular vesicles (EVs) from cell culture supernatant or plasma can be accomplished in a variety of ways. Common measures to quantify relative success are: concentration of the EVs, purity from non-EVs associated protein, size homogeneity and functionality of the final product. Here, we present an industrial-scale workflow for isolating highly pure and functional EVs using cross-flow based filtration coupled with high-molecular weight Capto Core size exclusion. Through this combination, EVs loss is kept to a minimum. It outperforms other isolation procedures based on a number of biochemical and biophysical assays. Moreover, EVs isolated through this method can be further concentrated down or directly immunopurified to obtain discreet populations of EVs. From our results, we propose that cross-flow/Capto Core isolation is a robust method of purifying highly concentrated, homogenous, and functionally active EVs from industrial-scale input volumes with few contaminants relative to other methods.

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“…The core has multimodal binding ligands that allow operation under various ranges of pH and NaCl concentrations. Recent work showed that the amount of ovalbumin was greatly reduced in a influenza A purification process (Blom et al, ); and the same resin was successfully used for the polishing step of canine adenovirus (Fernandes et al, ). One apparent limitation of negative mode is the difficulty to efficiently remove the impurities as compared to positive‐mode chromatography; thus, so far negative mode has been implemented only as a polishing step; however it could be also used as a virus removal tool as practiced in the mAb industry.…”

Section: Chromatographymentioning

confidence: 99%

Improved virus purification processes for vaccines and gene therapy

Nestola

Peixoto

Silva

et al. 2015

Biotech & Bioengineering

113

106

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The downstream processing of virus particles for vaccination or gene therapy is becoming a critical bottleneck as upstream titers keep improving. Moreover, the growing pressure to develop cost-efficient processes has brought forward new downstream trains. This review aims at analyzing the state-of-the-art in viral downstream purification processes, encompassing the classical unit operations and their recent developments. Emphasis is given to novel strategies for process intensification, such as continuous or semi-continuous systems based on multicolumn technology, opening up process efficiency. Process understanding in the light of the pharmaceutical quality by design (QbD) initiative is also discussed. Finally, an outlook of the upcoming breakthrough technologies is presented.

show abstract

Efficient chromatographic reduction of ovalbumin for egg-based influenza virus purification

Cited by 41 publications

References 10 publications

Enterovirus71 virus-like particles produced from insect cells and purified by multistep chromatography elicit strong humoral immune responses in mice

Enterovirus71 virus-like particles produced from insect cells and purified by multistep chromatography elicit strong humoral immune responses in mice

Large‐scale, cross‐flow based isolation of highly pure and endocytosis‐competent extracellular vesicles

Improved virus purification processes for vaccines and gene therapy

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