Efficient control system for PCR chips

Kim, J. D.; Kim, J.; Lee, Geumyoung

doi:10.1117/12.873572

Cited by 11 publications

(5 citation statements)

References 13 publications

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“…In this paper, we proposed a POC molecular diagnostic device capable of a web-based UI and easy maintenance, equipment operation, and monitoring [3]. The use of an open platform, an independent execution for each function, and the introduction of a REST API server facilitated software management.…”

Section: Discussionmentioning

confidence: 99%

“…The World Health Organization (WHO) proposed ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and robust, Equipment-free, and Deliverable to end-users) as a performance evaluation index for POC devices [1]. In this paper, the proposed system used an open platform using disposable polymerase chain reaction (PCR) chips [3]. POC equipment consumes a lot of time and human resources for maintenance, and this can be saved by using an open platform.…”

Section: Introductionmentioning

confidence: 99%

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System Architecture for IIoT-Based POC Molecular Diagnostic Device

Kil

Park²,

Park

et al. 2021

The 8th International Symposium on Sensor Science

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In this paper, we investigate an efficient structure for a point-of-care (POC) molecular diagnostic system based on the industrial Internet of things (IIoT). The target system can perform automated molecular diagnosis including DNA extraction, PCR amplification, and fluorescence detection. Samples and reagents are placed in a multi-room cartridge and loaded into the system. A rotating motor and a syringe motor control the cartridge to extract DNA from the sample. The extracted DNA is transferred to a polymerase chain reaction (PCR) chamber for DNA amplification and detection. The proposed system provides multiplexing of up to four colors. For POC molecular diagnostics, the World Health Organization demands features such as low volume, low cost, fast results, and a user-friendly interface. In this paper, we propose a system structure that can satisfy these requirements by using a PCR chip and open platform. A distributed structure is adopted for the convenience of maintenance, and a web-based GUI is adopted for the user’s convenience. We also investigated communication problems that may occur between system components. Using the proposed structure, the user can conveniently control from standard computing devices including a smartphone.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

System Architecture for IIoT-Based POC Molecular Diagnostic Device

Kil

Park²,

Park

et al. 2021

The 8th International Symposium on Sensor Science

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show abstract

“…The PCR chip's temperature is controlled in the local system through temperature measurement of the chip thermistor and the periodic control of the heater and fan. The local-host system using the PC has the advantage of reducing the total cost because major functions can be performed in the host [8,9,10,11].…”

Section: Black Pcb-based Micro-pcr Chipmentioning

confidence: 99%

Micro-Polymerase Chain Reaction Chip Construction for Fluorescence Detection Test

Hwang¹,

Kim²,

Song³

et al. 2015

Advanced Science and Technology Letters

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Abstract. This paper proposes the optimal structure of a Printed Circuit Boardbased micro-PCR chip constructed on a PCB substrate using commercial adhesive tapes and plastic films. The proposed micro-PCR chip was composed of four layers. In a previous experiment, the chip had been made with a green PCB, yielding an unwanted reflection that prevented accurate analysis. In this study, the solder mask of the PCB substrate was coated black for a higher signal-to-noise ratio, and the area where the reaction chamber was attached was legend-printed with white silk for better reflection of the fluorescence light. The top cover was also colored black to minimize the noise during fluorescence detection. As a result of carrying out PCR, it was found that the fluorescence brightness increased with increasing numbers of cycles.

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“…Because it was difficult to achieve proper resolution with the normal timer provided by the windows OS, a high precision event timer (HPET) was used to achieve a resolution smaller than 1 ms, which was to facilitate various multimedia applications. Note that the local-host system using the PC has the advantage of reducing the total cost because major functions can be performed in the host [9][10][11][12]. …”

Section: Pcr Chip Driving Systemmentioning

confidence: 99%