So far, there exist many publicly available palmprint databases. However, not all of them have provided the corresponding region of interest (ROI) images. If everyone uses their own extracted ROI images for performance testing, the final accuracy is not strictly comparable. Since ROI localization is the critical stage of palmprint recognition. The location precision has a significant impact on the final recognition accuracy, especially in unconstrained scenarios. This problem has limited the applications of palmprint recognition. However, many currently published surveys only focus on feature extraction and classification methods. Throughout these years, many new ROI localization methods have been proposed. In this chapter, we will group the existing ROI localization methods into different categories, analyze their basic ideas, reproduce some of the codes, make comparisons of their performances, and provide further directions. We hope this could be a useful reference for further research.