Efficient Delivery of dsRNA and DNA in Cultured Silkworm Cells for Gene Function Analysis Using PAMAM Dendrimers System

Lü, Chenchen; Li, Zhiqing; Chang, Li; Dong, Zhaoming; Guo, Pengchao; Shen, Guanghui; Xia, Qingyou

doi:10.3390/insects11010012

Cited by 13 publications

(7 citation statements)

References 34 publications

(42 reference statements)

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“…[ 146 ] Currently, the ability of vectors such as PAMAM dendrimers to deliver RNA to tumor cells has been well established. [ 147 ] Multiple in vivo and in vitro studies have proved that PAMAM dendrimers can successfully deliver genetic material (siRNA, miRNA, and plasmids) into cells and produce specific therapeutic effects, [ 130 ] such as silencing some protein genes and inhibiting the proliferation of tumor cells.…”

Section: Utilization Of Dendrimers In Cancermentioning

confidence: 99%

Polymeric Dendrimers as Nanocarrier Vectors for Neurotheranostics

Zha

Liu

et al. 2022

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immune responses, activating cellular machinery causing cell stress, cytotoxicity, lack of selectivity, increasing the likelihood of replication competence, and the constrained structure of insert-size, which can limit their biological and medical applications. [2][3][4] By contrast, various forms of nonviral vectors are widely developed and applied in biomedical research because of their low immunogenicity, high biocompatibility, simplicity in structural pattern, ease of size adjustment, practicality in designing various active sites, and low cost of production. [5,6] The most widely studied nonviral vectors are liposomes, inorganic nanoparticles, polymers, and dendrimers. [7] Liposomes are small spherical vesicles from cholesterol and phospholipids characterized with the surface that can be modified using glycolipids or sialic acid. Yet, the unmodified forms of liposomes are structurally unstable and could easily be eliminated by means of body circulation. [8] In this situation, stable inorganic nanoparticles, such as calcium phosphate (Ca 3 (PO 4 ) 2 ), gold (Au), iron oxide (Fe 3 O 4 ), and lanthanide-based nanoparticles are widely used, mainly due to their good hydrophilicity and excellent biocompatibility. [9][10][11] Depending on their chemical core composition, these inorganic nanoparticles display different properties and efficiencies. Lately, inorganic nanoparticles have been developed as effective drug delivery systems and used in many diagnostic techniques. [12] In addition, polymers are widely used as nonviral vectors as well. [13][14][15] Polymers are biodegradable nanomaterials with stable and easily modifiable 3D structures. Their uncomplicated synthesis process allows for large-scale production and safe clinical use compared to inorganic nanoparticles. [16] Notably, dendrimers are hyperbranched polymers with a well-defined chemical structure: a core at the center occupied by array of convergent reactive chain-ends and surface that can easily be modified. [17] The chemistry of dendrimers was first described by Buhleier et al. in 1978. [18] In 1985, Tomalia et al. reported a newer kind of dendrimers based on the mixture of amines and amides, termed polyamidoamine (PAMAM) dendrimers. [19] Dendrimers are intermeshed synthetic organic macromolecules with 3D architecture composition and abundant terminal functional groups. They are well-defined monodispersity-sized materials with extraordinary membrane interaction properties Dendrimers are polymers with well-defined 3D branched structures that are vastly utilized in various neurotheranostics and biomedical applications, particularly as nanocarrier vectors. Imaging agents can be loaded into dendrimers to improve the accuracy of diagnostic imaging processes. Likewise, combining pharmaceutical agents and anticancer drugs with dendrimers can enhance their solubility, biocompatibility, and efficiency. Practically, by modifying ligands on the surface of dendrimers, effective therapeutic and diagnostic platforms can be constructed and implemented for target...

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Section: Utilization Of Dendrimers In Cancermentioning

confidence: 99%

Polymeric Dendrimers as Nanocarrier Vectors for Neurotheranostics

Zha

Liu

et al. 2022

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“…The dsRNAs for control gene of Red were also synthesized. G5-PAMAMmediated delivery of dsRNA was used according to the previous protocol (45). G5-PAMAM was purchased from Chenyuan Company (Shandong, China).…”

Section: Rna Interferencementioning

confidence: 99%

“…We firstly synthesized specific dsRNAs against ORF and UTR of BmTCTP (referred to as dsTCTP-ORF and dsTCTP-UTR) (Figure 2A). G5-PAMAM system was used to deliver dsRNAs of BmTCTP into the BmN cells to silence BmTCTP expression (45). RT-PCR result showed that both dsTCTP-ORF and dsTCTP-UTR were able to decrease the mRNA transcription of BmTCTP compared with the dsRed control treatment (Figure 2B), and also dsTCTP-ORF could specifically decrease the expression of FLAG-TCTP by immunoblotting (Figure S1), which together revealed that dsRNAs of BmTCTP could efficiently silence its own expression.…”

Section: Bmtctp Knockdown Promoted Viral Replicationmentioning

confidence: 99%

SUMOylation of Translationally Regulated Tumor Protein Modulates Its Immune Function

Lü

Zhang

et al. 2022

Front. Immunol.

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Translationally controlled tumor protein (TCTP) is a highly conserved protein possessing numerous biological functions and molecular interactions, ranging from cell growth to immune responses. However, the molecular mechanism by which TCTP regulates immune function is largely unknown. Here, we found that knockdown of Bombyx mori translationally controlled tumor protein (BmTCTP) led to the increased susceptibility of silkworm cells to virus infection, whereas overexpression of BmTCTP significantly decreased the virus replication. We further demonstrated that BmTCTP could be modified by SUMOylation molecular BmSMT3 at the lysine 164 via the conjugating enzyme BmUBC9, and the stable SUMOylation of BmTCTP by expressing BmTCTP-BmSMT3 fusion protein exhibited strong antiviral activity, which confirmed that the SUMOylation of BmTCTP would contribute to its immune responses. Further work indicated that BmTCTP is able to physically interact with interleukin enhancer binding factor (ILF), one immune molecular, involved in antivirus, and also induce the expression of BmILF in response to virus infection, which in turn enhanced antiviral activity of BmTCTP. Altogether, our present study has provided a novel insight into defending against virus via BmTCTP SUMOylation signaling pathway and interacting with key immune molecular in silkworm.

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“…To address this challenge, a variety of delivery approaches have been developed by multidisciplinary research communities, including physical (thermoporation, electroporation, magnetoporation, mechanoporation, optoporation, sonoporation, and microinjection) strategies (Meacham et al., 2014 ; Du et al., 2018 ), chemical strategies (artificial lipids (Hou et al., 2022 ), dendrimers (Lu et al., 2019 ), polymers (Farshbaf et al., 2018 ), carbon nanotubes (Mangla et al., 2020 ) or nanoparticles and direct chemical modification such as PLGA (Matta & Maalouf, 2019 ) or cholesterol (Ruwizhi & Aderibigbe, 2020 )), and biological strategies (viral delivery (Lundstrom, 2020 ) and non-viral delivery (Bono et al., 2020 ) such as cell penetrating peptides (CPPs)) (Thompson et al., 2012 ; Liu et al., 2016 ; Geng et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Improved transfer efficiency of supercharged 36 + GFP protein mediate nucleic acid delivery

et al. 2022

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The potential of nucleic acid therapeutics to treat diseases by targeting specific cells has resulted in its increasing number of uses in clinical settings. However, the major challenge is to deliver bio-macromolecules into target cells and/or subcellular locations of interest ahead in the development of delivery systems. Although, supercharged residues replaced protein 36 + GFP can facilitate itself and cargoes delivery, its efficiency is still limited. Therefore, we combined our recent progress to further improve 36 + GFP based delivery efficiency. We found that the penetration efficacy of 36 + GFP protein was significantly improved by fusion with CPP-Dot1l or treatment with penetration enhancer dimethyl sulfoxide (DMSO) in vitro . After safely packaged with plasmid DNA, we found that the efficacy of in vitro and in vivo transfection mediated by 36 + GFP-Dot1l fusion protein is also significantly improved than 36 + GFP itself. Our findings illustrated that fusion with CPP-Dot1l or incubation with DMSO is an alternative way to synergically promote 36 + GFP mediated plasmid DNA delivery in vitro and in vivo .

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Efficient Delivery of dsRNA and DNA in Cultured Silkworm Cells for Gene Function Analysis Using PAMAM Dendrimers System

Cited by 13 publications

References 34 publications

Polymeric Dendrimers as Nanocarrier Vectors for Neurotheranostics

Polymeric Dendrimers as Nanocarrier Vectors for Neurotheranostics

SUMOylation of Translationally Regulated Tumor Protein Modulates Its Immune Function

Improved transfer efficiency of supercharged 36 + GFP protein mediate nucleic acid delivery

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